Cheese-whey permeate improves the fitness of Escherichia coli cells during recombinant protein production

Divitiis, Marcella de; Ami, Diletta; Pessina, Alex; Palmioli, Alessandro; Sciandrone, Barbara; Airoldi, Cristina; Regonesi, Maria Elena; Brambilla, Luca; Lotti, Marina; Natalello, Antonino; Brocca, Stefania; Mangiagalli, Marco

doi:10.1186/s13068-023-02281-8

Cited by 10 publications

(5 citation statements)

References 61 publications

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“…The composition of coproducts present in processed food can be evaluated by the FTIR spectroscopy technique, which demonstrates the structural differences in the molecules of different types of foods and determines the use and bioavailability of nutrients [58]. In this sense, using whey as an inducer of recombinant protein production by Escherichia coli improved the fitness and efficiency of protein expression without interfering with membrane fluidity and permeability [59]. Thus, the use of lactose as a carbon source, present in SL, by E. coli maximized the metabolism and microbial machinery due to the greater availability of micronutrients.…”

Section: Discussionmentioning

confidence: 99%

Interference with Bacterial Conjugation and Natural Alternatives to Antibiotics: Bridging a Gap

Guidotti-Takeuchi,

Melo,

Ribeiro

et al. 2023

Antibiotics

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Horizontal gene transfer (HGT) in food matrices has been investigated under conditions that favor gene exchange. However, the major challenge lies in determining the specific conditions pertaining to the adapted microbial pairs associated with the food matrix. HGT is primarily responsible for enhancing the microbial repertoire for the evolution and spread of antimicrobial resistance and is a major target for controlling pathogens of public health concern in food ecosystems. In this study, we investigated Salmonella Heidelberg (SH) and Escherichia coli (EC) regarding gene exchange under conditions mimicking the industrial environment, with the coproducts whey (SL) and chicken juice (CJ). The S. Heidelberg strain was characterized by antibiotic susceptibility standards and PCR to detect the blaTEM gene. A concentration of 0.39 mg/mL was determined to evaluate the anti-conjugation activity of nanostructured lipid nanocarriers (NLCs) of essential oils to mitigate β-lactam resistance gene transfer. The results showed that the addition of these coproducts promoted an increase of more than 3.5 (whey) and 2.5 (chicken juice) orders of magnitude in the conjugation process (p < 0.01), and NLCs of sage essential oil significantly reduced the conjugation frequency (CF) by 74.90, 90.6, and 124.4 times when compared to the transfers in the absence of coproducts and the presence of SL and CJ, respectively. For NLCs from olibanum essential oil, the decrease was 4.46-fold for conjugations without inhibitors and 3.12- and 11.3-fold in the presence of SL and CJ. NLCs associated with sage and olibanum essential oils effectively control the transfer of antibiotic resistance genes and are a promising alternative for use at industrial levels.

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Section: Discussionmentioning

confidence: 99%

Interference with Bacterial Conjugation and Natural Alternatives to Antibiotics: Bridging a Gap

Guidotti-Takeuchi,

Melo,

Ribeiro

et al. 2023

Antibiotics

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“…Compounds were manually identified with the support of 2D spectra (Supplementary Material-Figures S2 and S3) on a small pool of spectra. Then, a procedure already developed by our group for the analysis of metabolite mixtures from different types of samples was applied [22][23][24][25][26][27][28], in order to obtain the fast and semi-automatic identification and quantification of the metabolites present in all the Antarctic soil samples under examination. To this aim, a specific library, available as an .exp file [29], was created using the Simple Mixture Analysis (SMA) tool implemented in MestreNova 14.3.0-30573 software.…”

Section: Antarctic Soil Extract Preparation and Nmr-based Metabolic P...mentioning

confidence: 99%

Antarctic Soil Metabolomics: A Pilot Study

Ciaramelli

Palmioli

Brioschi

et al. 2023

IJMS

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In Antarctica, ice-free areas can be found along the coast, on mountain peaks, and in the McMurdo Dry Valleys, where microorganisms well-adapted to harsh conditions can survive and reproduce. Metabolic analyses can shed light on the survival mechanisms of Antarctic soil communities from both coastal sites, under different plant coverage stages, and inner sites where slow-growing or dormant microorganisms, low water availability, salt accumulation, and a limited number of primary producers make metabolomic profiling difficult. Here, we report, for the first time, an efficient protocol for the extraction and the metabolic profiling of Antarctic soils based on the combination of NMR spectroscopy and mass spectrometry (MS). This approach was set up on samples harvested along different localities of Victoria Land, in continental Antarctica, devoid of or covered by differently developed biological crusts. NMR allowed for the identification of thirty metabolites (mainly sugars, amino acids, and organic acids) and the quantification of just over twenty of them. UPLC-MS analysis identified more than twenty other metabolites, in particular flavonoids, medium- and long-chain fatty acids, benzoic acid derivatives, anthracenes, and quinones. Our results highlighted the complementarity of the two analytical techniques. Moreover, we demonstrated that their combined use represents the “gold standard” for the qualitative and quantitative analysis of little-explored samples, such as those collected from Antarctic soils.

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“…SJH cells to define if and how the process affects their cellular response and the overall macromolecular composition. This approach has been applied successfully to study changes induced by various stressors as for example the overproduction of recombinant proteins and protein aggregation (Doglia et al 2008 ; DeDivitiis et al 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Iron bioleaching and polymers accumulation by an extreme acidophilic bacterium

Marchetti,

Kupka,

Senatore

et al. 2024

Arch Microbiol

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In many European regions, both local metallic and non-metallic raw materials are poorly exploited due to their low quality and the lack of technologies to increase their economic value. In this context, the development of low cost and eco-friendly approaches, such as bioleaching of metal impurities, is crucial. The acidophilic strain Acidiphilium sp. SJH reduces Fe(III) to Fe(II) by coupling the oxidation of an organic substrate to the reduction of Fe(III) and can therefore be applied in the bioleaching of iron impurities from non-metallic raw materials. In this work, the physiology of Acidiphilium sp. SJH and the reduction of iron impurities from quartz sand and its derivatives have been studied during growth on media supplemented with various carbon sources and under different oxygenation conditions, highlighting that cell physiology and iron reduction are tightly coupled. Although the organism is known to be aerobic, maximum bioleaching performance was obtained by cultures cultivated until the exponential phase of growth under oxygen limitation. Among carbon sources, glucose has been shown to support faster biomass growth, while galactose allowed highest bioleaching. Moreover, Acidiphilium sp. SJH cells can synthesise and accumulate Poly-β-hydroxybutyrate (PHB) during the process, a polymer with relevant application in biotechnology. In summary, this work gives an insight into the physiology of Acidiphilium sp. SJH, able to use different carbon sources and to synthesise a technologically relevant polymer (PHB), while removing metals from sand without the need to introduce modifications in the process set up.

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Cheese-whey permeate improves the fitness of Escherichia coli cells during recombinant protein production

Cited by 10 publications

References 61 publications

Interference with Bacterial Conjugation and Natural Alternatives to Antibiotics: Bridging a Gap

Interference with Bacterial Conjugation and Natural Alternatives to Antibiotics: Bridging a Gap

Antarctic Soil Metabolomics: A Pilot Study

Iron bioleaching and polymers accumulation by an extreme acidophilic bacterium

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