“Checkerboard” Assessments of Periodontal Microbiota and Serum Antibody Responses: A Case‐Control Study

Papapanou, Panos N.; Neiderud, Anne‐Marie; Papadimitriou, Alexandra; Sandros, Jens; Dahlén, Gunnar

doi:10.1902/jop.2000.71.6.885

Cited by 108 publications

(143 citation statements)

References 63 publications

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“…46 The immune response to infection may also undergo age-related changes that result in loss of response and functional capacity. 47 Hence, we controlled for age and stratified on smoking status.…”

Section: Discussionmentioning

confidence: 99%

Periodontal Disease and Coronary Heart Disease

et al. 2005

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Background-Results from studies relating periodontal disease to cardiovascular disease have been mixed. Residual confounding by smoking and use of clinical measures of periodontal disease rather than measures of infection have been 2 major criticisms. The aims of this study were to investigate relationships between prevalent coronary heart disease (CHD) and 2 exposures, (1) clinical periodontal disease and (2) IgG antibodies to 17 oral organisms, and to evaluate the role of smoking in these relationships. Methods and Results-Our study is based on a subset of participants in the Atherosclerosis Risk in Communities (ARIC)Study, who received a complete periodontal examination during visit 4 (1996 -1998). The exposures were periodontal status and serum IgG antibody levels against 17 periodontal organisms, and the outcome was prevalent CHD at visit 4. Multivariable analyses indicate that periodontal status is not significantly associated with CHD in either ever smokers or never smokers. Similar analyses evaluating antibodies indicate that high antibodies (above the median) to Treponema denticola (odds ratio [OR]ϭ1.7; 95% CI, 1.2 to 2.3), Prevotella intermedia (ORϭ1.5; 95% CI, 1.1 to 2.0), Capnocytophaga ochracea (ORϭ1.5; 95% CI, 1.1 to 2.1), and Veillonella parvula (ORϭ1.7; 95% CI, 1.2 to 2.3) are significantly associated with CHD among ever smokers, whereas Prevotella nigrescens (ORϭ1.7; 95% CI, 1.1 to 2.6), Actinobacillus actinomycetemcomitans (ORϭ1.7; 95% CI, 1.2 to 2.7), and Capnocytophaga ochracea (ORϭ2.0; 95% CI, 1.3 to 3.0) were associated with CHD among never smokers. Conclusions-Clinical signs of periodontal disease were not associated with CHD, whereas systemic antibody response was associated with CHD in ever smokers and never smokers. These findings indicate that the quality and quantity of the host response to oral bacteria may be an exposure more relevant to systemic atherothrombotic coronary events than clinical measures.

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Section: Discussionmentioning

confidence: 99%

Periodontal Disease and Coronary Heart Disease

et al. 2005

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“…Because Porphyromonas gingivalis is considered the major periodontal pathogen implicated in periodontitis in adult populations, we explored for additional risk loci using its "high" colonization phenotype, defined as above, as a separate trait. Various approaches in defining the bacterial colonization profiles have been previously used, including summations of the absolute microbial counts (Offenbacher et al, 2007), tertile categorization (Desvarieux et al, 2005), and five-level categorization of log10-transformed counts (Papapanou et al, 2000). For the present investigation, we defined a "high" colonization trait as the top quintile (20%) of each trait's distribution.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide Association Study of Periodontal Pathogen Colonization

et al. 2012

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Pathological shifts of the human microbiome are characteristic of many diseases, including chronic periodontitis. To date, there is limited evidence on host genetic risk loci associated with periodontal pathogen colonization. We conducted a genome-wide association (GWA) study among 1,020 white participants of the Atherosclerosis Risk in Communities Study, whose periodontal diagnosis ranged from healthy to severe chronic periodontitis, and for whom “checkerboard” DNA-DNA hybridization quantification of 8 periodontal pathogens was performed. We examined 3 traits: “high red” and “high orange” bacterial complexes, and “high” Aggregatibacter actinomycetemcomitans (Aa) colonization. Genotyping was performed on the Affymetrix 6.0 platform. Imputation to 2.5 million markers was based on HapMap II-CEU, and a multiple-test correction was applied (genome-wide threshold of p < 5 × 10−8). We detected no genome-wide significant signals. However, 13 loci, including KCNK1, FBXO38, UHRF2, IL33, RUNX2, TRPS1, CAMTA1, and VAMP3, provided suggestive evidence (p < 5 × 10−6) of association. All associations reported for “red” and “orange” complex microbiota, but not for Aa, had the same effect direction in a second sample of 123 African-American participants. None of these polymorphisms was associated with periodontitis diagnosis. Investigations replicating these findings may lead to an improved understanding of the complex nature of host-microbiome interactions that characterizes states of health and disease.

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“…In the present study, we used the checkerboard DNA-DNA hybridization technique, which is considered an efficient method; moreover, it does not require bacterial viability and is particularly applicable in epidemiological research (Ali et al 1997, Papapanou et al 2000, Craig et al 2001, Dowsett et al 2002. However, this technique, based on whole genomic probes, may give cross-hybridization of bacteria with closely related species (Wong et al 1996).…”

Section: Limitations Of the Studymentioning

confidence: 99%

Tobacco Smoking and Periodontal Health in a Saudi Arabian Population

Natto

Baljoon

Bergström

2005

Journal of Periodontology

114

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Background & Aim: Tobacco smoking exerts a harmful effect on the periodontal tissues manifested by periodontal pockets, attachment loss and periodontal bone loss. Current evidences on the effects of tobacco on periodontal health mainly concern cigarette smoking. In view of the increasing popularity of water pipe smoking in Arabian countries and reports confirming that water pipe smoking has health effects similar to those of cigarette smoking, there is a need for a better understanding of the potential harm of this smoking habit. The present thesis was carried out in order to explore whether water pipe smoking is associated with periodontal health in a manner similar to cigarette smoking.

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“Checkerboard” Assessments of Periodontal Microbiota and Serum Antibody Responses: A Case‐Control Study

Abstract: A combined microbial colonization/antibody response profile can effectively discriminate between periodontitis patients and periodontally intact controls.

Cited by 108 publications

References 63 publications

Periodontal Disease and Coronary Heart Disease

Periodontal Disease and Coronary Heart Disease

Genome-wide Association Study of Periodontal Pathogen Colonization

Tobacco Smoking and Periodontal Health in a Saudi Arabian Population

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