“…LYVE-1 protein is robustly expressed by LECs during normal mouse development, including in the jugular lymph sacs at E12.5, and is also detected at some level in lymph nodes, liver sinusoids, spleen sinuses, blood vessels, endocardium and a subpopulation of macrophages (Banerji et al, 1999; Baluk and McDonald, 2008; Gordon et al, 2008). The Lyve-1 Cre mouse line has been used by others to successfully inactivate, in a lymphatic-targeted manner, the genes encoding sphingosine kinase, VEGFR2, and the chromatin remodeling enzyme CHD4 (Pham et al, 2010; Dellinger et al, 2013; Crosswhite et al, 2016). Dellinger et al showed that Lyve-1 Cre can be used to delete floxed DNA sequences in LECs that generate collecting lymphatics and their valves, as well as lymphatic capillaries (Dellinger et al, 2013).…”