This study was aimed to investigate the machining stability of a horizontal milling machine with hybrid guideway systems by finite element method. To this purpose, we first created finite element model of the milling machine with the introduction of the contact stiffness defined at the sliding and rolling interfaces, respectively. Also, the motorized built-in spindle model was created and implemented in the whole machine model. Results of finite element simulations reveal that linear guides with different preloads greatly affect the dynamic responses and machining stability of the horizontal milling machine. The critical cutting depth predicted at the vibration mode associated with the machine tool structure is about 10 mm and 25 mm in the X and Y direction, respectively, while the cutting depth predicted at the vibration mode associated with the spindle structure is about 6.0 mm. Also, the machining stability can be increased when the preload of linear roller guides of the feeding mechanism is changed from lower to higher amount.