Chasing bacteria within the cells using levofloxacin-loaded hyaluronic acid nanohydrogels

Montanari, Elita; D’Arrigo, Giorgia; Meo, Chiara Di; Virga, A.; Coviello, Tommasina; Passariello, Claudio; Matricardi, Pietro

doi:10.1016/j.ejpb.2014.03.003

Cited by 50 publications

(29 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…194 Levofloxacin-loaded hyaluronic acid hydrogels were reported to be able to chase bacteria within the cells for both S. aureus and P. aeruginosa strains. 127 In order to eradicate bacterial biofilm and avoid possible intestinal obstructions, Islan et al 195 reported a smart autodegradable hydrogel containing alginate lyase (AL) and levofloxacin, which induced the reduction of drug toxicity and enhancement of drug bioavailability. A hydrogel based on (−)-menthol, which is a traditional cooling compound tailed by an amino acid derivate through an alkyl chain, can provide innoxious environment to living cells and deliver lincomycin to the local infection site.…”

Section: Other Antibioticsmentioning

confidence: 99%

“…126 There are two main ways to overcome antibiotic resistance, one is manufacture of novel antibiotics, and the other is minimizing the dosage to decrease antibiotic resistance. 127 To achieve the second aim, local antibiotic administration has drawn increasing attention in recent years to improve the treatment effects. Antibioticloaded systems can deliver an adequate local bactericidal dose directly to the infected site, without significantly overtaking the systemic toxicity level.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial hydrogels: promising materials for medical application

Yang

Han

Chen

et al. 2018

IJN

304

230

View full text Add to dashboard Cite

The rapid emergence of antibiotic resistance in pathogenic microbes is becoming an imminent global public health problem. Local application of antibiotics might be a solution. In local application, materials need to act as the drug delivery system. The drug delivery system should be biodegradable and prolonged antibacterial effect should be provided to satisfy clinical demand. Hydrogel is a promising material for local antibacterial application. Hydrogel refers to a kind of biomaterial synthesized by a water-soluble natural polymer or a synthesized polymer, which turns into gel according to the change in different signals such as temperature, ionic strength, pH, ultraviolet exposure etc. Because of its high hydrophilicity, unique three-dimensional network, fine biocompatibility and cell adhesion, hydrogel is one of the suitable biomaterials for drug delivery in antimicrobial areas. In this review, studies from the past 5 years were reviewed, and several types of antimicrobial hydrogels according to different ingredients, different preparations, different antimicrobial mechanisms, different antimicrobial agents they contained and different applications, were summarized. The hydrogels loaded with metal nanoparticles as a potential method to solve antibiotic resistance were highlighted. Finally, future prospects of development and application of antimicrobial hydrogels are suggested.

show abstract

Section: Other Antibioticsmentioning

confidence: 99%

mentioning

confidence: 99%

Antimicrobial hydrogels: promising materials for medical application

Yang

Han

Chen

et al. 2018

IJN

304

230

View full text Add to dashboard Cite

show abstract

“…We successfully developed an HA gel sheet that exists as a gel despite containing no gel-forming component other than HA. Although it has been reported that HA gels can be prepared by click chemistry, enzymatic crosslinking, disulfide crosslinking, radical polymerization, condensation reactions, and functionalization of HA with hydrophobic molecules [15][16][17][18][19][20], reports of HA gels obtained by noncovalent interactions of unmodified HA are scarce. In the present study, viscosity measurements using a rotational viscometer indicate that phosphoric acid plays a key role in the HA gelation in our formulation, because the viscosity of HA increased after addition of phosphoric acid.…”

Section: Discussionmentioning

confidence: 99%

“…HA gel sheets are widely used for medical treatment such as wound healing and bone regeneration [13,14]. They are usually prepared by crosslinking HA via chemical modification [15][16][17][18][19][20], but this modified HA is not natural and its manufacturing process is complicated. For the efficient transdermal delivery of ALN, the aim of this study was to develop an HA gel sheet that is produced simply by noncovalent interactions.…”

Section: Introductionmentioning

confidence: 99%

Development of a Phosphoric Acid-Mediated Hyaluronic Acid Gel Sheet for Efficient Transdermal Delivery of Alendronate for Anti-Osteoporotic Therapy

et al. 2019

View full text Add to dashboard Cite

For efficient transdermal delivery of alendronate (ALN) for anti-osteoporotic therapy, we developed a hyaluronic acid (HA) gel sheet that was prepared simply by enhancing HA noncovalent interactions using phosphoric acid and polyhydric alcohol (propanediol and glycerin). HA solution viscosity increased after addition of phosphoric acid, and the HA gel sheet formed after heated drying. The HA gel sheet could be converted to high viscosity state by addition of water. These results indicate that phosphoric acid enhances the noncovalent interactions of HA molecules. The HA gel sheet elicited no skin irritation over 7 days after a 24-h application. The permeation of ALN across rat and human skin was 109 and 7.17 µg/cm2, respectively, up to 24 h after application of the ALN-loaded HA gel sheet, which is sufficient for clinical treatment of osteoporosis. The bioavailability of ALN in rats was ~20% after application of the ALN-loaded HA gel sheet, and plasma calcium levels were effectively reduced 3 days after sheet application. Furthermore, in a rat osteoporosis model, the reduction in tibial bone density was suppressed by treatment with the ALN-loaded HA gel sheet. These results indicate that our phosphoric acid-mediated HA gel sheet is a promising transdermal formulation for efficient ALN delivery.

show abstract

“…This approach can open the way for the treatment of infections caused by obligate or facultative intracellular macrophage pathogens, which are becoming a serious health problem . Indeed HA‐based formulations have already shown efficacy for the targeted and intracellular delivery of antimicrobials, as it has been demonstrated using levofloxacin‐loaded nanoparticles prepared from amphiphilic HA‐cholesteryl derivatives …”

Section: Introductionmentioning

confidence: 99%

Hyaluronan/Tannic Acid Nanoparticles Via Catechol/Boronate Complexation as a Smart Antibacterial System

Montanari

Gennari

Pelliccia

et al. 2016

Macromolecular Bioscience

View full text Add to dashboard Cite

Nanoparticles based on hyaluronic acid (HA) are designed to deliver tannic acid (TA) as an antimicrobial agent. The presence of HA makes these particles potentially useful to target bacteria that colonize cells presenting HA membrane receptors (e.g. CD44), such as macrophages. HA bearing 3-aminophenyl boronic acid groups (HA-APBA) is reacted with TA, yielding nanoparticles with a size that decreases with decreasing HA molecular weight (e.g. 200 nm for 44 kDa, 400 nm for 737 kDa). The boronate esters make the nanoparticles stable at physiological pH, but their hydrolysis in an acidic environment (pH = 5) leads to swelling/solubilization, therefore potentially allowing TA release in endosomal compartments. We have assessed the nanoparticle toxicity profile (on RAW 264.7 macrophages) and their antimicrobial activity (on E. coli and on both methicillin-sensitive and -resistant S. aureus). The antibacterial effect of HA-APBA/TA nanoparticles was significantly higher than that of TA alone, and has very similar activity to TA coformulated with a reducing agent (ascorbic acid), which indicates both the nanoparticles to protect TA catechols from oxidation, and the effective release of TA after nanoparticle internalization. Therefore, there is potential for these nanoparticles to be used in stable, effective, and potentially targetable nanoparticle-based antimicrobial formulations.

show abstract

Chasing bacteria within the cells using levofloxacin-loaded hyaluronic acid nanohydrogels

Cited by 50 publications

References 35 publications

Antimicrobial hydrogels: promising materials for medical application

Antimicrobial hydrogels: promising materials for medical application

Development of a Phosphoric Acid-Mediated Hyaluronic Acid Gel Sheet for Efficient Transdermal Delivery of Alendronate for Anti-Osteoporotic Therapy

Hyaluronan/Tannic Acid Nanoparticles Via Catechol/Boronate Complexation as a Smart Antibacterial System

Contact Info

Product

Resources

About