Charles Asher on Secrets

Asher, Charles

doi:10.1080/19342039.2015.1083829

Cited by 3 publications

(4 citation statements)

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“…Excitation in resonance with these transitions leads to an enhancement (even by a factor of 10 3 to 10 5 ) of the Raman‐active modes of a porphyrin . The excitation within the Soret maxima provides strong enhancement, mainly of the ν 4 band . On the other hand, with the use of the excitation wavelength of 532 nm we observe the enhancement of the ν 19 and ν 10 bands, which are connected with encompassing the Q band of the visible spectrum …”

Section: Resultsmentioning

confidence: 73%

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Raman spectroscopy of proteins: a review

Ryguła

Majzner

Marzec

et al. 2013

J Raman Spectroscopy

878

790

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In this work, 26 proteins of different structure, function and properties are investigated by Raman spectroscopy with 488, 532 and 1064 nm laser lines. The excitation lines were chosen in NIR and Vis range as the most common and to show the difference due to normal and resonance effect, sometimes accompanied by the fluorescence. The selected proteins were divided, according to the Structural Classification of Proteins, into four classes according to their secondary structure, i.e. α‐helical (α), β‐sheet (β), mixed structures (α/β, α + β, s) and others. For all compounds, FT‐Raman and two Vis spectra are presented along with the detailed band assignment. To the best of our knowledge, this is the first review showing the potential of Raman spectroscopy for the measurement and analysis of such a large collection of individual proteins. This work can serve as a comprehensive vibrational spectra library, based on our and previous Raman measurements. Copyright © 2013 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 73%

“…The electronic absorption spectrum of porphyrins is characterized by two bands which are called Soret maximum (or Soret band, at around at 420 nm) and Q maximum (or Q band, at around 550 nm) . Excitation in resonance with these transitions leads to an enhancement (even by a factor of 10 3 to 10 5 ) of the Raman‐active modes of a porphyrin .…”

Section: Resultsmentioning

confidence: 99%

Raman spectroscopy of proteins: a review

Ryguła

Majzner

Marzec

et al. 2013

J Raman Spectroscopy

878

790

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“…This method provides information about the prosthetic group of the protein and the oxidation and spin states of the heme iron species . Excitation within the Soret maxima of Hb gives strong enhancement mainly of the ν 4 band, while the enhancement of ν 19 and ν 10 bands is connected with exciting into the Qv band of the visible spectrum of Hb . Resonance Raman imaging also enables the visualization of 2D and 3D distributions of heme in both cells and tissues .…”

Section: Introductionmentioning

confidence: 99%

High‐resolution Raman imaging reveals spatial location of heme oxidation sites in single red blood cells of dried smears

Marzec

Ryguła

Wood

et al. 2014

J Raman Spectroscopy

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Raman confocal microscopy with 488 nm excitation wavelength supported with atomic force microscopy (AFM), scanning near‐field optical microscopy (SNOM) and UV–Vis spectrometry was used to investigate air‐dried erythrocytes (red blood cells, RBCs) in whole human blood smears. The central internal part of the cell was dominated by the laser‐induced O2 dissociated oxyHb form as evidenced by the Fe2+ marker band appearing at 1356 cm−1. The existence of a thin outer layer of hemoglobin in the periphery of RBCs was assigned to hemichrome. Evidence for hemichrome includes the oxidation state marker band appearing at 1376 cm−1, the absence of FeO2 band at 570 cm−1 and a UV–Vis spectrum consistent with hemichrome. This is the first time that distributions of Fe2+/Fe3+ hemes inside the single RBC have been reported. The outer layer formation of hemichrome was additionally studied when RBCs were in contact with leucocytes and carotenoids of blood plasma. Copyright © 2014 John Wiley & Sons, Ltd.

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“…ProudToParent (Asher & Asher, n.d.) is a free online parent education program for separating parents who were never married to each other. In a recent 2-year period, more than 15,000 parents used ProudToParent in the United States (Asher & Asher, 2015), and it is used in many of the courtrooms in the county where the current study was conducted. However, there is no empirical research on its efficacy.…”

mentioning

confidence: 99%

Randomized control trial: Online parent program and waiting period for unmarried parents in Title IV-D court.

Rudd¹,

Holtzworth‐Munroe²,

Reyome³

et al. 2015

Journal of Family Psychology

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Despite a lack of research on parent education programs for unmarried parents, many judicial officers mandate participation. We recruited an understudied sample likely at high risk for negative outcomes-182 court cases involving unmarried parents on government assistance in which paternity was contested and then established via genetic testing ordered by the court. This 2 × 2 randomized controlled trial evaluated the impact on initial litigation outcomes of two factors: (a) participation in an online parent education program or not and (b) having a waiting period between the establishment of paternity and the court hearing concerning child-related issues or not. Using an intent-to-treat framework, we found that among cases not assigned to the program, there was no difference in the rate of full agreement on child-related issues (e.g., child support, custody, parenting time) when comparing cases assigned to a waiting period and cases not assigned to a waiting period. In contrast, for cases assigned to the program, cases also assigned a waiting period were less likely to reach a full agreement than cases that had their hearing on the same day. In addition, cases in the "program and waiting period" condition were less likely to return to court for their hearing than cases in the "no program and waiting period" condition. In exploratory analyses of the subsample of cases in which both parents were present at the court hearing, the pattern of results remained the same, although the findings were no longer statistically significant.

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Charles Asher on Secrets

Cited by 3 publications

References 0 publications

Raman spectroscopy of proteins: a review

Raman spectroscopy of proteins: a review

High‐resolution Raman imaging reveals spatial location of heme oxidation sites in single red blood cells of dried smears

Randomized control trial: Online parent program and waiting period for unmarried parents in Title IV-D court.

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