Chargen- und gerätespezifische Kalibrierung der Thromboplastinzeit-Bestimmung: Ein Beitrag zur Qualitätssicherung im Gerinnungslabor

Spanuth, E.; Breyer, Juan Manuel; Bablock, W.

doi:10.1515/labm.1984.8.5.147

Cited by 4 publications

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“…The low sensitivity of the standard test within the important area between normal and subnormal values can partly be attributed to the fact that it already has in humans its highest sensitivity in the scope 15-25 YO coagulation activity, which is important for the surveillance of oral anticoagulant therapy, whereas between 100 and 50 % only 4 4 . 5 s difference occurs (SPANUTH et al, 1984;BARTHELS et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

“…An even higher sample dilution is disadvantageous because with this, coagulation activities C50 % already produce very long coagulation times (>I20 s) which are difficult to reproduce. In order to get a PT result which is independent of the reagent charge, as in humans (SPANUTH et al, 1984) the coagulation activity (Yo) should be calculated based on a PFP calibration curve. The reference range is then 59-148 % for a 1 :2O sample dilution.…”

Section: Discussionmentioning

confidence: 99%

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Influence of Sample Predilution on the Sensitivity of Prothrombin Time in Feline Plasma

Mischke

Denız

Nolte

1996

Journal of Veterinary Medicine Series A

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The prothrombin time (PT) is measured in feline plasma usually by means of a method that has been optimized for measurements in human plasma (standard test). In the present study the sensitivity of this test was investigated in comparison to a modified PT using a human placental thromboplastin. In the modified test, 100 fl sample predilution (1 :5, 1 : 10, and 1 :20) were incubated with 100 pl of a human fibrinogen solution (2 g/l) until 100 pl Ca-thromboplastin was added. When measured in 31 samples with a reduced, and 22 samples with an increased activity of the coagulation factors 11, V, VII and/or X the modified test showed an increased sensitivity as well as a correlation between PT and the changes in single factor activity which was distinctly closer compared to the standard test. The highest sensitivity was produced by a 1 : 10 or 1 :20 sample dilution. A test procedure modified in this way, is thus, a more reliable screening test for the extrinsic system in cats and should be used for routine diagnostics.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Influence of Sample Predilution on the Sensitivity of Prothrombin Time in Feline Plasma

Mischke

Denız

Nolte

1996

Journal of Veterinary Medicine Series A

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“…; Die Kontrollplasmen PreciCIot® l/l l und PreciChrom® Fib i l/l l wurden in 17fach-Bestimmung in der Serie und an . 10 (13). Die Verlängerung der Gerinnungszeiten durch erhöhte Fibrin(ogen)-Spaltprodukte im koagulometrischen-, nicht jedoch in photometrischen Test stellt eine weitere Ursache aufgetretener Divergenzen dar.…”

Section: C) Methodenvergleichunclassified

Applikation photometrischer Tests zur Bestimmung von Thromboplastinzeit (TPZ), aktivierter partieller Thromboplastinzeit (aPTT) und Fibrinogen an den Analysenautomaten Gem-Profiler

Ratge¹,

Wisser²

1988

LaboratoriumsMedizin

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The application ofthe routine coagulation parameters thromboplastin time (TPZ), activated partial thromboplastin time (aPTT) and fibrinogen to the Gern-ProfHer centrifugalanalyzer ispresented. Instead of detection of the first fibrin Strands during clotting äs a measurement point, the photometric TPZ and aPTT methods relies on the measurement of the colored product p-nitroanilin at 405 nm. This colored product is cleaved out of the chromogenic Substrate by thrombin activated through the coagulation cascade. The kinetic assay for fibrinogen is based on aggregation of des-A-fibrin to fibrin S following proteolytic cleavage of fibrinogen with a solution of the thrombin-like snake venom batroxobin. Clotting velocity is recorded turbidimetrically at340nm, For the photometric coagulation tests coefficients of Variation from 0.7 to 4.8% within run and from 1.5 to 6.1% from day to day in control samples in the normal and abnormal ränge are comparable to that of mechanised assays of clinical chemistry. The correlations between the photometric and the coagulometric methods in a heterogenous patient group showed good agreements of the methods (r = 0.91-0.97). Lipemic specimens, hemolysis and bilirubin do notinterfere with fibrinogen determinations whereas bilirubin levels higher than 400 / Interrupt the measurement of TPZ and aPTT. The advantages of the photometric TPZ-and aPTTmethods are: fibrinogen-independent measurement, very good precision, easy performänce f 1 Electro Nucleonics International, Stuttgart. 232Lab.med. 12: 232 (1988) Brought to you by | University of Queensland -UQ Library Authenticated Download Date | 7/13/15 2:11 AM standardization and automatisation (sample throughput of 120-160/h). The mean features of the kinetic turbidimetric method for determination offunctional fibrinogen in comparison to the Clauss-technique are the broad measuring ränge without necessary sample dilutions, the better reliability and the Iower interference by fibrin/fibrinogen degradation products.

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“…unterschiedliche Faktorenempfindlichkeit etc. Verbesserungsmöglichkeiten bieten sich an durch die Standardisierung der Reagenzien (24), durch den Einsatz teiloder vollmechanisierter Analysengeräte und durch die konsequente Anwendung zuverlässiger Verfahren zur Kalibrierung insbesondere der Thromboplastinzeit -oder der Fibrinogenbestimmung (18). Eine entscheidende Verbesserung läßt jedoch erst die Einführung neuer gerinnungsanalytischer Global tests für Thromboplastinzeit und partielle Thromboplastinzeit auf der Basis chromogener Substrate und die damit verbundene Einführung des photometrischen Meßprinzips erwarten (20)(21)(22)(23).…”

Section: Introductionunclassified

Ergebnisse zur statistischen Qualitätskontrolle gerinnungsanalytischer Routinemethoden im Rahmen des Qualitäts-Control-Service (Q.C.S.)-Gerinnung

Spanuth¹,

Breyer²

1987

LaboratoriumsMedizin

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Zusammenfassung: Mit dem Qualitäts-Control-Service (Q.C.S.)-Gerinnung wird ein System zur statistischen Qualitätskontrolle für die Methoden der Gerinnungsanalytik vorgestellt. Grundlage ist das Universalkontrollplasma PreciCIot®, mit Sollwerten im normalen und abnormalen Bereich. Da das Kontrollplasma von allen Teilnehmern täglich für die Qualitätskontrolle mitgeführt wird und die Daten monatlich ausgewertet werden, führt der Qualitäts-Control-Service (Q.C.S.)-Gerinnung zu ähnlichen Aussagen wie die Teilnahme an einem sich wiederholenden Ringversuch. Es wird über die Ergebnisse einer Kontrollperiode (Januar-Dezember 1985) berichtet, in der die Daten von 75 Laboratorien für die Routinemethoden Thromboplastinzeit nach Quick, aktivierte partielle Thromboplastinzeit (APTT), Fibrinogen nach Clauss.und Thrombinzeit ausgewertet wurden. Die ermittelten Kenngrößen für Richtigkeit und Präzision der Methoden werden angegeben und mit den Ergebnissen früherer Ringversuche verglichen. Anhand der Daten wird die interlaboratorielle Vergleichbarkeit der Methoden diskutiert und die Vorteile des Qualitäts-Control-Service (Q.C.S.)-Gerinnung für eine bessere Beurteilung der Qualität gerinnungsanalytischer Resultate aufgezeigt. Schlüsselwörter: Qualitäts-Control-Service (Q.C.S.)-Gerinnung -Gerinnungsanalysen -Qualitätskontrolle -Ringversuch -UniversalkontrollplasmaSummary: With the Quality-Control-Service (Q.C.S.)-Coagulation a system for the statistical quality control of coagulation methods is presented. The system is based on the universal control plasma PreciCIot® which contains target values in the normal and abnormal range. As the control plasma is used daily from the participants for quality control and dates are statistically analyzed each month, Q.C.S.-Coagulation can be compared with a monthly ring trial. For the methods prothrombin time (PT/Quick), activated partial prothrombin time (APTT), fibrinogen assay (Fibrinogen) and thrombin time (Thrombin) dates of one control period (January-December 1985) with 75 labs were evaluated. Calculated results for the methods are given and accuracy and precision of the methods are compared with the results of former ring trials. Based on the results the interlaboratory reliability of the methods is discussed and the advantages of Q. C.S. -Coagulation for a better information about quality of coagulation tests are presented.

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Chargen- und gerätespezifische Kalibrierung der Thromboplastinzeit-Bestimmung: Ein Beitrag zur Qualitätssicherung im Gerinnungslabor

Cited by 4 publications

References 0 publications

Influence of Sample Predilution on the Sensitivity of Prothrombin Time in Feline Plasma

Influence of Sample Predilution on the Sensitivity of Prothrombin Time in Feline Plasma

Applikation photometrischer Tests zur Bestimmung von Thromboplastinzeit (TPZ), aktivierter partieller Thromboplastinzeit (aPTT) und Fibrinogen an den Analysenautomaten Gem-Profiler

Ergebnisse zur statistischen Qualitätskontrolle gerinnungsanalytischer Routinemethoden im Rahmen des Qualitäts-Control-Service (Q.C.S.)-Gerinnung

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