Charge translocation by mitochondrial NADH:ubiquinone oxidoreductase (complex I) from Yarrowia lipolytica measured on solid-supported membranes

ND3 Cys39 in complex I is exposed during mitochondrial respiration

“…The catalytic A/D transition occurs in vertebrates and in fungi containing complex I ( Neurospora crassa and Yarrowia lipolytica ) ( Agip et al, 2018 ; Blaza et al, 2018 ; Fiedorczuk et al, 2016 ; Gorenkova et al, 2013 ; Grba and Hirst, 2020 ; Grivennikova et al, 2003 ; Kalashnikov et al, 2011 ; Maklashina et al, 2002 , 2003 , 2004 ; Siebels and Dröse, 2016 ; Zhu et al, 2016 ). In contrast, complex I of invertebrate metazoans as well as bacteria ( Paracoccus denitrificans, Thermus thermophilus ) does not undergo a catalytic A/D transition ( Jarman et al, 2021 ; Kotlyar et al, 1998 ; Maklashina et al, 2003 ), although it has been claimed to occur for the Escherichia coli enzyme ( Belevich and Verkhovskaya, 2016 ; Belevich et al, 2017a , 2017b ).…”