Charge Transference Detection through Cyclic Voltammograms in the Interaction of Cratylia mollis Seed Lectin-carbohydrate Inhibitor

Abstract

Aims: To evaluate the charge transference and the electrochemical potential of Cratylia mollis seed lectin, Cramoll 1,4, adsorbed on Nafion beads after the interaction of glucose ligand.

Biosensor Characterization from Cratylia mollis Seed Lectin (Cramoll)‐MOF and Specific Carbohydrate Interactions in an Electrochemical Model

“…Cyclic voltammetry (VC) is a powerful technique to probe the electrochemical properties of organic molecules; [53] it has been used in the detection of glucose without the presence of lectin, [54] and with the presence of Cramoll [55] …”

“…We used the biosensor technique to assess the relationship between the response current and glucose concentration; electrochemical current monitoring assesses lectin‐carbohydrate binding using this amperometric method [55,56] . It is observed in the CV graph ( Figure 4) that the standard analysis (in buffered medium) shows a linear increase in current, even after 0.0 V of potential, thus not showing biological interactions [53] .…”

“…We used the biosensor technique to assess the relationship between the response current and glucose concentration; electrochemical current monitoring assesses lectin-carbohydrate binding using this amperometric method. [55,56] It is observed in the CV graph (Figure 4) that the standard analysis (in buffered medium) shows a linear increase in current, even after 0.0 V of potential, thus not showing biological interactions. [53] While the samples with glucose showed an exponential increase in the cathodic current, at peak 1, representing the binding site of the monosaccharide, in the potential range from À 0.2 to 0.0 V; with the current reaching 0.0003 A, after 0.0 V the current decreases.…”

“…[14,35,51,52] Cramoll-Glucose Cyclic Voltammetry Cyclic voltammetry (VC) is a powerful technique to probe the electrochemical properties of organic molecules; [53] it has been used in the detection of glucose without the presence of lectin, [54] and with the presence of Cramoll. [55] It was verified that there is no change in the electrical current in a cyclic voltammogram with the gold disk containing only the carbon paste and different concentrations of glucose (10, 15 and 20 mM) in an electrolytic medium of 20 mM potassium phosphate buffer, pH 7.0. After the addition of MOF in this carbon paste, the spectra presented in the cyclic voltammogram (Figure 3) showed a change in the anodic and cathodic peaks, indicating the possibility of this system being used in the construction of the biosensor after the immobilization of a biomolecule on the surface of this modified electrode.…”