Charge binding of rhodamine derivative to OH− stabilized nanomaghemite: Universal nanocarrier for construction of magnetofluorescent biosensors

Magro, Massimiliano; Sinigaglia, Giulietta; Nodari, Luca; Tuček, Jiří; Poláková, Kateřina; Marušák, Zdeněk; Cardillo, Sara; Salviulo, Gabriella; Russo, Umberto; Stevanato, Roberto; Zbořil, Radek; Vianello, Fábio

doi:10.1016/j.actbio.2012.02.005

Cited by 68 publications

(73 citation statements)

References 64 publications

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“…As shown in Figure 2, the particles have a globular shape with a size distribution of ~10-20 nm (transmission electron microscopy imaging). Based on gas analysis and spectral data from Fourier transform infrared spectroscopy published in our previous work, 18 we could confirm that the surface of the particles interacted with surrounding water molecules, maintaining low interparticle forces and good colloidal stability.…”

Section: Characterization Of Samn Nanoparticlessupporting

confidence: 52%

“…Detailed description of the synthesis and the precise physical and chemical characterization of these maghemite nanoparticles are described in our previous paper. 18 Magnetic relaxometry of SAMNs was performed with a 3 T MRI scanner (Siemens Skyra, Erlangen, Germany) at the specialized experimental laboratory at Graz University, Graz, Austria. For all measurements, a circular polarized coil ("animal-coil") with an inner diameter of 7.5 cm (RAPID Biomedical GmbH, Rimpar, Germany) was used to obtain a high signal-to-noise ratio.…”

Section: Materials and Methods Synthesis And Characterization Of Samnmentioning

confidence: 99%

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Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist – an initial in vitro study

Skopalík¹,

Poláková²,

Havrdová³

et al. 2014

IJN

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Objective Cell therapies have emerged as a promising approach in medicine. The basis of each therapy is the injection of 1–100×10 6 cells with regenerative potential into some part of the body. Mesenchymal stromal cells (MSCs) are the most used cell type in the cell therapy nowadays, but no gold standard for the labeling of the MSCs for magnetic resonance imaging (MRI) is available yet. This work evaluates our newly synthesized uncoated superparamagnetic maghemite nanoparticles (surface-active maghemite nanoparticles – SAMNs) as an MRI contrast intracellular probe usable in a clinical 1.5 T MRI system. Methods MSCs from rat and human donors were isolated, and then incubated at different concentrations (10–200 μg/mL) of SAMN maghemite nanoparticles for 48 hours. Viability, proliferation, and nanoparticle uptake efficiency were tested (using fluorescence microscopy, xCELLigence analysis, atomic absorption spectroscopy, and advanced microscopy techniques). Migration capacity, cluster of differentiation markers, effect of nanoparticles on long-term viability, contrast properties in MRI, and cocultivation of labeled cells with myocytes were also studied. Results SAMNs do not affect MSC viability if the concentration does not exceed 100 μg ferumoxide/mL, and this concentration does not alter their cell phenotype and long-term proliferation profile. After 48 hours of incubation, MSCs labeled with SAMNs show more than double the amount of iron per cell compared to Resovist-labeled cells, which correlates well with the better contrast properties of the SAMN cell sample in T2-weighted MRI. SAMN-labeled MSCs display strong adherence and excellent elasticity in a beating myocyte culture for a minimum of 7 days. Conclusion Detailed in vitro tests and phantom tests on ex vivo tissue show that the new SAMNs are efficient MRI contrast agent probes with exclusive intracellular uptake and high biological safety.

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Section: Characterization Of Samn Nanoparticlessupporting

confidence: 52%

Section: Materials and Methods Synthesis And Characterization Of Samnmentioning

confidence: 99%

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist – an initial in vitro study

Skopalík¹,

Poláková²,

Havrdová³

et al. 2014

IJN

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View full text Add to dashboard Cite

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“…A typical nanoparticle synthesis has already been described 11,12 and can be summarized as follows: FeCl 3 × 6H 2 O (10.0 g, 37 mmol) was dissolved in MilliQ grade water (800 mL) under vigorous stirring at room temperature. An NaBH 4 solution (2 g, 53 mmol) in ammonia (3.5%, 100 mL) was then quickly added to the mixture.…”

Section: Synthesis Of Iron Oxide Nanoparticlesmentioning

confidence: 99%

“…The resulting nanoparticulated material was characterized by zero field and in field (5T) Mossbauer spectroscopy, FTIR spectroscopy, high-resolution TEM, X-ray powder diffraction, magnetization measurements, 11,12 and constituted stoichiometric maghemite (γ-Fe 2 O 3 ) with a mean diameter of 10 ± 2 nm. Sonication in water (mod.…”

Section: Synthesis Of Iron Oxide Nanoparticlesmentioning

confidence: 99%

“…They also present a high average magnetic moment and can be easily derivatized to immobilize specific organic molecules in a solution. 11,12 The aim of this present work is to combine the advantages of immobilized proteins on nanomaterials and the easy operation of magnetic nanoparticles to develop an inexpensive and simple amine oxidase-based magnetic nanocatalyst tagged with a fluorescent probe.…”

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confidence: 99%

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Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles

Sinigaglia¹,

Magro²,

Miotto³

et al. 2012

IJN

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Novel superparamagnetic surface-active maghemite nanoparticles (SAMNs) characterized by a diameter of 10 ± 2 nm were modified with bovine serum amine oxidase, which used rhodamine B isothiocyanate (RITC) adduct as a fluorescent spacer-arm. A fluorescent and magnetically drivable adduct comprised of bovine serum copper-containing amine oxidase (SAMN-RITC-BSAO) that immobilized on the surface of specifically functionalized magnetic nanoparticles was developed. The multifunctional nanomaterial was characterized using transmission electron microscopy, infrared spectroscopy, mass spectrometry, and activity measurements. The results of this study demonstrated that bare magnetic nanoparticles form stable colloidal suspensions in aqueous solutions. The maximum binding capacity of bovine serum amine oxidase was approximately 6.4 mg g -1 nanoparticles. The immobilization procedure reduced the catalytic activity of the native enzyme to 30% ± 10% and the Michaelis constant was increased by a factor of 2. We suggest that the SAMN-RITC-BSAO complex, characterized by a specific activity of 0.81 IU g -1, could be used in the presence of polyamines to create a fluorescent magnetically drivable H 2 O 2 and aldehydes-producing system. Selective tumor cell destruction is suggested as a potential future application of this system.

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Fluorescent Composite Nano‐ and Microparticles Based on Xanthene Dyes and Iron Oxides

Muradova,

Al‐Hilali,

Andreeva

et al. 2024

Chem Eng & Technol

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Modern industry actively uses metal structural materials to create innovative devices and components. In order to prevent emergency situations in production, nondestructive testing methods are used to detect defects on the surface of the material being developed. Among the methods of nondestructive testing, one can highlight the method of luminescent magnetic flaw detection, which allows identifying defects on the surface and under the surface of the test sample with minimal effort. It is known that in some cases, the magnetic‐powder method cannot achieve the required sensitivity due to the shape and location of defects themselves or due to certain conditions of object operation. To increase the visibility and sensitivity, magnetic nano‐ and microparticles can be conjugated with the dyes. Iron oxide nanoparticles (Fe3O4, γ‐Fe2O3) can be used as such magnetic particles, since they are quite easy to obtain and have low toxicity, high chemical stability, and a wide variety of magnetic properties. To ensure fluorescence of magnetic particles, a wide variety of fluorescent dyes associated with such particles are used. The dyes can include morin, naphthalimide derivatives, as well as semiconductor quantum dots. This article provides a review of fluorescent composite micro‐ and nanoparticles based on xanthene dyes (rhodamine, fluorescein, and pyronine). Such dyes are economically available luminophores, have good binding to the surface of magnetic particles, and therefore can be suitable for luminescent magnetic flaw detection. This review article discusses methods for producing composite nano‐ and microparticles of iron oxides with xanthene dyes and provides prospects of using composite nano‐ and microparticles in nondestructive testing methods.

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Charge binding of rhodamine derivative to OH− stabilized nanomaghemite: Universal nanocarrier for construction of magnetofluorescent biosensors

Cited by 68 publications

References 64 publications

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist – an initial in vitro study

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist – an initial in vitro study

Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles

Fluorescent Composite Nano‐ and Microparticles Based on Xanthene Dyes and Iron Oxides

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Charge binding of rhodamine derivative to OH− stabilized nanomaghemite: Universal nanocarrier for construction of magnetofluorescent biosensors

Cited by 68 publications

References 64 publications

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist &ndash; an initial in vitro study

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist &ndash; an initial in vitro study

Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles

Fluorescent Composite Nano‐ and Microparticles Based on Xanthene Dyes and Iron Oxides

Contact Info

Product

Resources

About

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist – an initial in vitro study

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist – an initial in vitro study