Charakterisierung der passiven Tubenöffnung in einer hypo-/hyperbaren Druckkammer

Meyer, Moritz; Mikolajczak, Stefanie; Lüers, Jan-Christoffer; Lotfipour, Shahram; Beutner, Dirk; Jumah, Masen Dirk

doi:10.1055/s-0033-1347175

Cited by 7 publications

(3 citation statements)

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“…The combination with bioinformatic network analyses is an integrative technology to analyse the change of innumerable proteins at a defined time [ 10 , 11 ]. Biological pathways can represent complex processes at molecular level and can be a valuable aid for computational and experimental research utilizing the Omics data [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Thirty Minutes of Hypobaric Hypoxia Provokes Alterations of Immune Response, Haemostasis, and Metabolism Proteins in Human Serum

Hinkelbein

Jansen

Iovino

et al. 2017

IJMS

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Hypobaric hypoxia (HH) during airline travel induces several (patho-) physiological reactions in the human body. Whereas severe hypoxia is investigated thoroughly, very little is known about effects of moderate or short-term hypoxia, e.g. during airline flights. The aim of the present study was to analyse changes in serum protein expression and activation of signalling cascades in human volunteers staying for 30 min in a simulated altitude equivalent to airline travel. After approval of the local ethics committee, 10 participants were exposed to moderate hypoxia (simulation of 2400 m or 8000 ft for 30 min) in a hypobaric pressure chamber. Before and after hypobaric hypoxia, serum was drawn, centrifuged, and analysed by two-dimensional gel electrophoresis (2-DIGE) and matrix-assisted laser desorption/ionization followed by time-of-flight mass spectrometry (MALDI-TOF). Biological functions of regulated proteins were identified using functional network analysis (GeneMania®, STRING®, and Perseus® software). In participants, oxygen saturation decreased from 98.1 ± 1.3% to 89.2 ± 1.8% during HH. Expression of 14 spots (i.e., 10 proteins: ALB, PGK1, APOE, GAPDH, C1QA, C1QB, CAT, CA1, F2, and CLU) was significantly altered. Bioinformatic analysis revealed an association of the altered proteins with the signalling cascades “regulation of haemostasis” (four proteins), “metabolism” (five proteins), and “leukocyte mediated immune response” (five proteins). Even though hypobaric hypoxia was short and moderate (comparable to an airliner flight), analysis of protein expression in human subjects revealed an association to immune response, protein metabolism, and haemostasis

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Section: Introductionmentioning

confidence: 99%

Thirty Minutes of Hypobaric Hypoxia Provokes Alterations of Immune Response, Haemostasis, and Metabolism Proteins in Human Serum

Hinkelbein

Jansen

Iovino

et al. 2017

IJMS

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“…Furthermore, the ETOP varied greatly depending on the collective of subjects and maneuver performed during the measurement. For example, the average value of healthy subjects was 22.7 hPa for the Frenzel maneuver, 17 to 41.2 hPa for the Valsalva maneuver,15.1 to 29.9 hPa for the Toynbee maneuver, and 30.2 to 41 hPa for passive tube opening (16,33–35).…”

Section: Discussionmentioning

confidence: 99%

Concordance Analysis of the Pressure Chamber and Tubomanometer According to Estève for the Determination of Eustachian Tube Opening Pressure

Deuss,

Löding,

Breitrück

et al. 2024

Otol Neurotol

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Introduction For the diagnosis of Eustachian tube dysfunction (ETD), clinical procedures such as tympanometry, micro-otoscopy, and maneuvers according to Toynbee and Valsalva only allow an indirect assessment for the moment. With a prevalence of up to 5%, the selection of patients with ETD and its subtypes is clinically relevant. Dynamic methods of Eustachian tube function assessment include a hypo/hyperbaric pressure chamber and Estève’s tubomanometer (TMM). One method of assessing ETD is the evaluation of Eustachian tube opening pressure (ETOP). Material and Methods We performed a concordance analysis between pressure chamber and TMM to determine ETOP. For this purpose, we analyzed the measurements of both methods from 28 healthy subjects using Bland–Altman plots, regression according to Passing–Bablok and Lin’s concordance correlations coefficient. The maximum tolerated clinical deviation of measured values was set at 10%. Results A maximum of 53 measurements of ETOP between pressure chamber and TMM were compared. Mean ETOP for TMM was 28.7 hPa, passive opening was 32 hPa, Toynbee maneuver was 28.4 hPa, and Valsalva maneuver was 54.6 hPa. Concordance analysis revealed following results: passive opening versus TMM: Bland–Altman mean difference 3.3 hPa, limits of agreement ±31.8 hPa; Passing–Bablok regression y = 0.67x + 9.36; Lin’s r ccc = 0.18. Toynbee versus TMM: Bland–Altman mean difference 0.7 hPa, limits of agreement ±35.8 hPa; Passing–Bablok regression y = 0.47x + 14.03; Lin’s r ccc = 0.14. Valsalva versus TMM: Bland–Altman mean difference 24.2 hPa, limits of agreement ±117.5 hPa; Passing–Bablok regression y = 0.17x + 25.12; Lin’s r ccc = 0.18. Conclusion Estève‘s tubomanometer and pressure chamber measurements of ETOP are not concordant. The two methods cannot be interchanged without reservation.

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“…Most active opening is involuntary and triggered by a need to swallow, however opening may also be stimulated by an increased pressure differential between baroreceptor or mechanoreceptors in the TM, ME and nasopharyngeal mucosa 13, 14, 15. Passive distension occurs if the nasopharyngeal or ME pressure exceeds the periluminal pressures that hold the lumen closed, 10 , 16 due either to ambient pressure change 17 or patient-generated pressures, such as during a Valsalva (forcibly exhaling with the nose and mouth occluded) 18 …”

Section: Introductionmentioning

confidence: 99%

Advances in Eustachian tube function testing

Smith

Bance

Tysome

2019

World j. otorhinolaryngol.-head neck surg.

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Obstructive and patulous Eustachian tube dysfunction provide a significant diagnostic and management challenge. The development of new treatments such as balloon Eustachian tuboplasty has generated renewed interest in measuring Eustachian tube function, as a method of selecting appropriate patients for intervention, and measuring their treatment outcomes.This review summarises recent findings relating to Eustachian tube function assessment. Increasingly it is recognised that patient reported outcome measures based on symptoms are highly non-specific and non-diagnostic, and clinical assessment alone may not permit the selection of individuals with abnormal Eustachian tube opening. Tests of Eustachian tube opening therefore may represent a practical and objective addition to patient assessment in clinic, allowing the identification of individuals with abnormal (patulous or obstructive) Eustachian tube function. A diagnostic pathway is described on this basis.More work is required to validate the described Eustachian tube function tests, and there remain individuals, such as those with dysfunction limited to pressure challenges, in whom function tests have yet to fully characterise the disorder.

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Charakterisierung der passiven Tubenöffnung in einer hypo-/hyperbaren Druckkammer

Abstract: The fundamental characterization of the action of the passive tube opening, including the measurement of the ETOP, ETCP and ETOD, is a first step in understanding the physiological and pathophysiological function of the ET.

Cited by 7 publications

References 1 publication

Thirty Minutes of Hypobaric Hypoxia Provokes Alterations of Immune Response, Haemostasis, and Metabolism Proteins in Human Serum

Thirty Minutes of Hypobaric Hypoxia Provokes Alterations of Immune Response, Haemostasis, and Metabolism Proteins in Human Serum

Concordance Analysis of the Pressure Chamber and Tubomanometer According to Estève for the Determination of Eustachian Tube Opening Pressure

Advances in Eustachian tube function testing

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