Characters, functions and clinical perspectives of long non-coding RNAs

Wu, Ruifang; Y, Su; Wu, Haijian; Dai, Yong; Zhao, Ming; Lu, Qianjin

doi:10.1007/s00438-016-1179-y

Cited by 68 publications

(52 citation statements)

References 165 publications

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“…Due to their versatility, as well as their diverse and elegantly controlled roles as key regulators in every level of gene expression, lncRNAs have been recognised as essential players both in normal cell physiology and numerous diseases and disorders, including a variety of cancers, and are currently being thoroughly investigated [34], [35]. lncRNAs are involved, among others, in the regulation of cell survival, apoptosis [34], [36] and migration [37].…”

Section: Discussionmentioning

confidence: 99%

“…These findings highlight the crucial role of MIAT in a variety of cancer-promoting processes in neuroblastoma and glioblastoma pathogenesis. However, further research is essential to establish that MIAT could be used as a biomarker in neuroblastoma and GBM patient samples in the future, like numerous other lncRNAs for a variety of tumours [34], [35], [57] and open new prognostic, predictive and even therapeutic avenues for patients suffering from these tumours . 755 756 757 758 759 760 761 762 763 764 765 766 767 768 769 770 771 772 773 774 775 776 777 778 779 780 781 782 783 784 785 786 787 788 789 790 791 792 793 794 795 796 797 798 799 800 801 802 803 804 805 806 807 808 809 810 811 812 814 815 816 817 818 819 820 821 822 823 82...…”

Section: Discussionmentioning

confidence: 99%

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RNA sequencing reveals a key role for the long non-coding RNA MIAT in regulating neuroblastoma and glioblastoma cell fate

Bountali

Tonge

Mourtada-Maarabouni

2019

International Journal of Biological Macromolecules

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Myocardial Infraction Associated Transcript (MIAT) is a subnuclear lncRNA that interferes with alternative splicing and is associated with increased risk of various heart conditions and nervous system tumours. The current study aims to elucidate the role of MIAT in cell survival, apoptosis and migration in neuroblastoma and glioblastoma multiforme. To this end, MIAT was silenced by MIAT-specific siRNAs in neuroblastoma and glioblastoma cell lines, and RNA sequencing together with a series of functional assays were performed. The RNA sequencing has revealed that the expression of an outstanding number of genes is altered, including genes involved in cancer-related processes, such as cell growth and survival, apoptosis, reactive oxygen species (ROS) production and migration. Furthermore, the functional studies have confirmed the RNA sequencing leads, with our key findings suggesting that MIAT knockdown eliminates long-term survival and migration and increases basal apoptosis in neuroblastoma and glioblastoma cell lines. Taken together with the recent demonstration of the involvement of MIAT in glioblastoma, our observations suggest that MIAT could possess tumour-promoting properties, thereby acting as an oncogene, and has the potential to be used as a reliable biomarker for neuroblastoma and glioblastoma and be employed for prognostic, predictive and, potentially, therapeutic purposes for these cancers.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

RNA sequencing reveals a key role for the long non-coding RNA MIAT in regulating neuroblastoma and glioblastoma cell fate

Bountali

Tonge

Mourtada-Maarabouni

2019

International Journal of Biological Macromolecules

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“…Long non-coding RNAs (lncRNAs) are kind of RNA molecule with a length of longer than 200 nucleotides and have no ability of coding protein [3]. As existing research results suggested that several lncRNAs are involved in transcriptional regulation, organization of nuclear structure, and post-transcriptional processing.…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer

Wang

et al. 2016

Biol Res

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Background and objectiveLong non-coding RNAs can regulate tumorigenesis of various cancers. Dys-regulation of lncRNA-AFAP1-AS1 has not been studied in colorectal carcinoma (CRC). This study was to examine the function involvement of AFAP1-AS1 in tumor growth and metastasis of CRC.MethodsRelative expression of AFAP1-AS1 in CRC tissues and CRC cells lines was determined using quantitative real-time PCR (qRT-PCR). Functional involvement of AFAP1-AS1 in tumor proliferation and metastasis was evaluated in AFAP1-AS1-specific siRNA-treated CRC cells and in CRC cell xenograft. Expression of epithelial-mesenchymal transition (EMT)-related gene expression was determined using western blot.ResultsRelative expression of AFAP1-AS1 was significantly elevated in CRC tissues and CRC HCT116 and SW480 cell lines. AFAP1-AS1 knock-down suppressed SW480 cell proliferation, colony formation, migration and invasion. Also AFAP1-AS1 knock-down inhibited tumor metastasis-associated genes expression in terms of EMT. This carcinostatic action by AFAP1-AS1 knock-down was further confirmed by suppression of tumor formation and hepatic metastasis of CRC cells in nude mice.ConclusionlncRNA-AFAP1-AS1 knock-down exhibits antitumor effect on colorectal carcinoma in respects of suppression of cell proliferation and metastasis of cancer cells.

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“…Recent studies have found a diverse population of lncRNAs with different sizes and functions in different species. These populations are expressed dynamically and act as important regulators in a variety of biological functions, especially in gene expression [32]. Accumulating evidence indicate that lncRNAs, by interacting with DNA, RNA, protein molecules and/or their combinations, play a critical role in regulation of cellular processes including genetic imprinting, chromatin remodeling, splicing regulation, mRNA decay, transcription, and translational regulation [33-36].…”

Section: Lncrnas In Npcmentioning

confidence: 99%

Functions and Roles of Long-Non-Coding RNAs in Human Nasopharyngeal Carcinoma

Hann²

2018

Cell Physiol Biochem

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Nasopharyngeal carcinoma (NPC) is one of the most common cancers originating in the nasopharynx and occurring at high frequency in South-eastern Asia and North Africa. Long non-coding RNAs (lncRNAs) are a class of non-protein-coding RNA molecules and key regulators of developmental, physiological, and pathological processes in humans. Emerging studies have shown that lncRNAs play critical roles in tumorgenicity and cancer prognosis. With the development of deep sequencing analyses, an extensive amount of functional lncRNAs have been discovered in nasopharyngeal carcinoma tissues and cell lines. However, the roles and mechanisms of aberrantly expressed lncRNAs in the pathogenesis of NPC are not fully understood. In this review, we briefly illustrate the concept, identification, functional characterization, and summarize recent advancements of biological functions of lncRNAs with heterogeneous mechanistic characterization and their involvement in NPC. Then, we describe individual lncRNAs that have been associated with tumorgenesis, growth, invasion, cancer stem cell differentiation, metastasis, drug resistance and discuss the strategies of their therapeutic manipulation in NPC. We also review the emerging insights into the role of lncRNAs and their potential as biomarkers and therapeutic targets for novel treatment paradigms. Finally, we highlight the up-to-date of clinical information involving lncRNAs and future directions in the linking lncRNAs to potential gene therapies, and how modifications of lncRNAs can be exploited for prevention and treatment of NPC.

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Characters, functions and clinical perspectives of long non-coding RNAs

Cited by 68 publications

References 165 publications

RNA sequencing reveals a key role for the long non-coding RNA MIAT in regulating neuroblastoma and glioblastoma cell fate

RNA sequencing reveals a key role for the long non-coding RNA MIAT in regulating neuroblastoma and glioblastoma cell fate

Long non-coding RNA AFAP1-AS1 facilitates tumor growth and promotes metastasis in colorectal cancer

Functions and Roles of Long-Non-Coding RNAs in Human Nasopharyngeal Carcinoma

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