Characterizzation of the 5' and 3' ends of the 16S ribonucleic acid from T1-ribonuclease-treated 30S ribosomes

Santer, Melvin; Santer, Ursula V.

doi:10.1021/bi00755a017

Cited by 12 publications

(18 citation statements)

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“…We had previously shown that band 5' emerges in molar quantities from Ti RNasetreated 30S ribosomes (23). When 70S ribosomes are used as substrate, it appears from preliminary estimates that band 5' also appears in molar quantities.…”

Section: Resultsmentioning

confidence: 96%

“…These RNA fragments represent areas that are completely resistant to Ti RNase hydrolysis under these experimental conditions. The bands are designated according to our previous numbering system (23,24) and include the following components. (i) Band 5' is a fragment of an RNA molecule that is about 120 nucleotides long and includes the 5' end of the 16S rRNA.…”

Section: Resultsmentioning

confidence: 99%

“…Column 1 represents RNA from 30S ribosomes treated with buffer 5. The various fractions have been previously characterized (23,24). Column 2 represents RNA from 30S ribosomes treated with buffer 10.…”

Section: Resultsmentioning

confidence: 99%

“…The growth of Escherichia coli MRE 600 on 32p medium and the isolation of 70, 30, and 50S ribosomes and the RNA from them have been previously described (23). The various buffers used are as follows.…”

Section: Methodsmentioning

confidence: 99%

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Area of 16S ribonucleic acid at or near the interface between 30S and 50S ribosomes of Escherichia coli

Santer¹,

S²

1977

J Bacteriol

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Area of 16S ribonucleic acid at or near the interface between 30S and 50S ribosomes of Escherichia coli

Santer¹,

S²

1977

J Bacteriol

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“…The region at the 3' terminus (the 3' half of section J) appears as a fragment about 25 nucleotides in length. The RNA derived from sections A and J accounts for more than 50% of the low-molecular-weight RNA generated from RNase T1treated ribosomes (29)(30)(31).…”

mentioning

confidence: 99%

Comparative Surface Structure of 16S Ribosomal Ribonucleic Acid of 30S Ribosomes of Procaryotic Cells

et al. 1979

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Ribonuclease T, treatment of 30S ribosomes of Escherichia coli converts a large region at the 3' OH end of 16S ribosomal ribonucleic acid (rRNA) to lowmolecular-weight RNA. The final 25 nucleotides at the 3' terminus of the molecule emerge relatively intact, whereas most of the region "upstream," for about 150 nucleotides, is converted to oligonucleotides. Identical enzyme treatment generates a fragment of about 60 nucleotides from the middle of 16S rRNA (section D'). To determine whether there are similar sequences in other bacteria, which occupy similar accessible surface locations, we treated 30S ribosomes from Azotobacter vinelandii and Bacillus stearothermophilus with RNase Ti. In each case, a fragment of RNA about 25 nucleotides in length containing the 3' OH end of 16S rRNA and a fragment of about 60 nucleotides in length similar, but not identical, in oligonuleotide composition to section D' of E. coli 16S rRNA were obtained from nuclease-treated 30S ribosomes. These data indicate that, although the primary structure at the 3' end and the middle (section D') of the various 16S rRNA's is not completely conserved, their respective conformations are conserved. A nuinber of identical oligonucleotides were found in the low-molecular-weight fraction obtained from RNase Ti-treated E. coli, A. vinelandii, and B. stearothermophilus 30S ribosomes. These results show that identical RNase T,-sensitive sequences are present in all three bacteria. Hydrolysis of these regions leads to the production of the fragments 25 and 60 nucleotides in length.on August 5, 2020 by guest http://jb.asm.org/ Downloaded from 132 the colicini E3 fragment: and (iii) analysis of the partial RNase T, digestion products of the colicin E3 region.

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The Role of Ribonucleic Acids in the Organization and Functioning of Ribosomes of E. coli

Bogdanov

Копылов

Shatsky

1980

Subcellular Biochemistry

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Characterizzation of the 5' and 3' ends of the 16S ribonucleic acid from T1-ribonuclease-treated 30S ribosomes

Cited by 12 publications

References 12 publications

Area of 16S ribonucleic acid at or near the interface between 30S and 50S ribosomes of Escherichia coli

Area of 16S ribonucleic acid at or near the interface between 30S and 50S ribosomes of Escherichia coli

Comparative Surface Structure of 16S Ribosomal Ribonucleic Acid of 30S Ribosomes of Procaryotic Cells

The Role of Ribonucleic Acids in the Organization and Functioning of Ribosomes of E. coli

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