Characterizing Winter Wheat Germplasm for Fusarium Head Blight Resistance Under Accelerated Growth Conditions

Zakieh, Mustafa; Gaikpa, David Sewordor; Sandoval, Fernanda Leiva; Alamrani, Marwan; Henriksson, Tina; Odilbekov, Firuz; Chawade, Aakash

doi:10.3389/fpls.2021.705006

Cited by 6 publications

(16 citation statements)

References 68 publications

(127 reference statements)

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“…The evaluated visual scores of the symptoms associated with FHB-bleached, yellowish or discolored, and stunted spikeswere previously validated by the identification of several loci by genome-wide association studies (GWAS) (Appendix 1), in a previous study with the same plants and visual scorings (Zakieh et al, 2021). The proposed methods aim to replace costly and labor-intensive genetic analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Wheat kernel samples were collected from an experiment under accelerated indoor growth conditions ( Zakieh et al., 2021 ) using winter wheat genotypes from two different sources. The first source consisted of 338 genotypes (breeding set) provided by the Swedish agricultural cooperative (Lantmännen Lantbruk, Svalöv, Sweden).…”

Section: Methodsmentioning

confidence: 99%

“…Finally, macroconidia spores were collected to make the inoculation suspension with the provided concentration after adding the surfactant Tween ® 20 0.002% (v/v) final volume of the inoculum. A more detailed protocol is described in Zakieh et al. (2021) .…”

Section: Methodsmentioning

confidence: 99%

“…Quantitative trait loci (QTL) detected in genome-wide association studies employing seven models at p = 0.0001 (LOD ≥ 4) for Fusarium head blight severity in winter wheat from the breeding, genebank, and combined sets (Zakieh et al, 2021). Chr., chromosome; FAF, favorable allele frequencies.…”

Section: Data Availability Statementmentioning

confidence: 99%

“…Traditionally, studies on FHB resistance have relied on measuring the symptoms in spikes and kernels (resistance types II and III). Type II is assessed by rating the visual symptoms on the spikes, which appear as bleached, yellowish or discolored, and stunted ( Zakieh et al., 2021 ; Steed et al., 2022 ). FDK is quantified traditionally by estimating the amount of visibly damaged kernels, which appear smaller, shriveled, and in a range of colors from pale pink to brown ( Delwiche et al., 2010 ), according to a predetermined scale for visual assessments or by employing manual tools ( Ackerman et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Phenotyping Fusarium head blight through seed morphology characteristics using RGB imaging

Leiva

Zakieh²,

Alamrani³

et al. 2022

Front. Plant Sci.

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Fusarium head blight (FHB) is an economically important disease affecting wheat and thus poses a major threat to wheat production. Several studies have evaluated the effectiveness of image analysis methods to predict FHB using disease-infected grains; however, few have looked at the final application, considering the relationship between cost and benefit, resolution, and accuracy. The conventional screening of FHB resistance of large-scale samples is still dependent on low-throughput visual inspections. This study aims to compare the performance of two cost–benefit seed image analysis methods, the free software “SmartGrain” and the fully automated commercially available instrument “Cgrain Value™” by assessing 16 seed morphological traits of winter wheat to predict FHB. The analysis was carried out on a seed set of FHB which was visually assessed as to the severity. The dataset is composed of 432 winter wheat genotypes that were greenhouse-inoculated. The predictions from each method, in addition to the predictions combined from the results of both methods, were compared with the disease visual scores. The results showed that Cgrain Value™ had a higher prediction accuracy of R2 = 0.52 compared with SmartGrain for which R2 = 0.30 for all morphological traits. However, the results combined from both methods showed the greatest prediction performance of R2 = 0.58. Additionally, a subpart of the morphological traits, namely, width, length, thickness, and color features, showed a higher correlation with the visual scores compared with the other traits. Overall, both methods were related to the visual scores. This study shows that these affordable imaging methods could be effective to predict FHB in seeds and enable us to distinguish minor differences in seed morphology, which could lead to a precise performance selection of disease-free seeds/grains.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Data Availability Statementmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenotyping Fusarium head blight through seed morphology characteristics using RGB imaging

Leiva

Zakieh²,

Alamrani³

et al. 2022

Front. Plant Sci.

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Reducing the generation time in winter wheat cultivars using speed breeding

et al. 2023

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Reducing generation time is critical to achieving the goals of genetic gain in important crops like wheat (Triticum aestivum). Speed breeding (SB) has been shown to considerably reduce generation times in crop plants. Unlike spring wheat cultivars, winter wheat varieties require typically 6-9 weeks of cold treatment, called vernalization, for flowering which extends the generation time for the development of improved winter wheat cultivars. Here, we optimized the SB method using a set of 48 diverse soft red winter wheat (SRWW) cultivars by testing vernalization duration, light and temperature requirements, and the viability of seeds harvested after different durations post-anthesis under extended daylight conditions. We have found that using a 22-h setting (22 h day/2 h night, 25˚C/22˚C) in high-density 50-cell trays results in rapid generation advancement. We used genotypic data for a panel of soft red winter wheat varieties from the regional programs to determine the impact of photoperiod and vernalization alleles on the efficiency of the SB approach. Using a set of 48 SRWW cultivars and germplasm from Maryland and four other public breeding programs, we establish that this protocol can allow for the advancement of four generations per year in controlled conditions for winter wheat varieties, experimental lines, or emerging cultivars. Our work shows the potential to reduce generation time Abbreviations: DH, doubled haploids; DPF, days post flowering; HID, high intensity discharge; SB, speed breeding; SSD, single seed descent.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Evaluation of speed breeding conditions for accelerating Fusarium head blight and deoxynivalenol screening in wheat

Chhabra,

Thrasu,

Wallace

et al. 2024

Crop Science

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Feeding the world's ever‐increasing population requires continuous development of high‐yielding and disease‐resistant cultivars of food crops such as wheat (Triticum aestivum L.). Speed breeding, which utilizes longer photoperiod times and higher temperatures, is a technique that accelerates plant development and is rapidly being adopted by wheat breeders across the globe to fast‐track cultivar development. Plant diseases are a major threat to crop production, and breeding for disease resistance is a major goal of crop breeders. Fusarium head blight (FHB), caused by Fusarium graminearum, is a major disease of small grain cereals, affecting their yield and quality. The aim of present work was to assess if speed breeding conditions can be used to accelerate reliable assessment of FHB severity and mycotoxin deoxynivalenol (DON) accumulation in wheat varieties. We screened a set of six spring wheat genotypes with different levels of genetic resistance (two moderately susceptible, two highly susceptible, one moderately resistant, and one resistant) for their response to FHB at 14 days after inoculation (dai) and 21 dai and DON accumulation under normal versus speed breeding conditions. FHB severity and DON accumulation were found to be highly correlated at all time points under normal and speed breeding conditions. Robust differentiation between resistant and susceptible genotypes could be achieved at 14 dai rather than the normal period of 21 dai, saving at least a week in phenotyping. Combined with the accelerated growth, flowering, and maturity under these conditions, efficient FHB screening and DON evaluation under speed breeding conditions will fast‐track development of resistant wheat varieties.

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Characterizing Winter Wheat Germplasm for Fusarium Head Blight Resistance Under Accelerated Growth Conditions

Cited by 6 publications

References 68 publications

Phenotyping Fusarium head blight through seed morphology characteristics using RGB imaging

Phenotyping Fusarium head blight through seed morphology characteristics using RGB imaging

Reducing the generation time in winter wheat cultivars using speed breeding

Evaluation of speed breeding conditions for accelerating Fusarium head blight and deoxynivalenol screening in wheat

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