Accurately determining the wall-shear-stress, τw , experimentally is challenging due to small spatial scales and large velocity gradients present in the near-wall region of turbulent flows. To avoid these resolution requirements, several indirect iterative fitting methods, most notably the Clauser chart method, exist for determining τw by fitting the mean velocity profile further away from the near-wall region in the log-law layer. These methods often require proper selection of fitting constants, assumptions of a canonical flow state, and other empirical based generalizations. To reduce the amount of ambiguity, determining the near-wall velocity gradient by assuming a linear relationship between the mean streamwise velocity and wall normal distance in the viscous sublayer can be used. However, this requires an accurate unbiased measurement of the near wall velocity profile in the region below five viscous spatial units, which can be less than 50 μm for high Reynolds number flows. Therefore, in this study a method for a volumetric defocusing micro particle tracking velocimetry method is presented that is capable of resolving the flow in the viscous sublayer of a turbulent boundary layer up to Ue = 44.7 m/s (Reθ = 27250). This method allows for the measurement of the near-wall flow through a single optical access for illumination and imaging and serves as an excellent complement of larger scale measurements that require near-wall information. The τw values determined from defocusing approach was found to be in good agreement values obtained from a simultaneous parallax PTV measurement. Furthermore, analysis of the diagnostic plot and cumulative distribution of measured fluctuations in the near-wall region, showed that both methods are capable of accurately determining mean velocity and fluctuation profiles in the self-similar viscous sublayer region.