Characterizing the structure/function parameter space of hydrocarbon-conjugated branched polyethylenimine for DNA delivery in vitro

Delivery of plasmid encoding interleukin-12 gene into hepatocytes by conjugated polyethylenimine-based nanoparticles

“…Despite the grafting of the moieties containing negative charge on the PEI surface, the net charge of the polyplexes was still positive which is necessary for the cell entry through the interaction between the positively charged polyplexes and the negative surface of the cell membranes. The results of the particle size and zeta potential measurements is consistent with the investigations in which the conjugation of various hydrophobic moieties into the PEI via its surface amines increases the particle size and leads to a decrease in the positive surface charge of the polyplexes (Dehshahri et al 2012, Doody et al 2006, Forrest et al 2004, Nimesh et al 2007, Oskuee et al 2008.…”

“…Based on the previous investigations, substitution of surface PEI amines by pendant moieties reduced the DNA binding affinity of the polymer particularly at lower polymer concentrations while no substantial difference was observed between the unmodified PEI and its conjugates at higher C/P ratios. Since the conjugation of primary surface amines reduced the ability of polymers to interact electrostatically with pDNA, more polymer was needed for the formation of tight complexes (Doody et al 2006, Forrest et al 2004, Gabrielson and Pack 2006, Nimesh et al 2007, Sabahi et al 2015.…”

Methylated N-aryl chitosan derivative/DNA complex nanoparticles for gene delivery: Synthesis and structure–activity relationships

“…It has been reported that hydrophobic modifications of cationic polymer, lipid and chitosan could assist better transfection capability as an optimal substitution (Chae, Son, Lee, Jang, & Nah, 2005;Doody, Korley, Dang, Zawaneh, & Putnam, 2006). This is expected due to increasing cell membrane/carrier interactions or the destabilization of the cell membranes, easier DNA unpacking from carriers and increasing of entry into cells, facilitation of endocytic uptake, and increasing of escape of complexes from endosome (Chae et al, 2005;Doody et al, 2006;Tian et al, 2007). In the present study, the introduction of N,N-dimethylaminocinnamyl and N,N-dimethylaminobenzyl moieties into the Ch backbone was expected to enhance the hydrophobicity of the carriers.…”

Synthetic vectors for gene delivery: An overview of their evolution depending on routes of administration.

“…A grafting ratio of 16% of N-acryloyl-L-histidine on lPEI corresponds to an optimal balance between transfection efficacy and cytotoxicity in comparison with lPEI. Moreover, replacement of some of the amino groups of PEI generally reduces toxic side effects of the corresponding polyplexes [65,66].…”