Characterizing the structural transition of cationic DPPC liposomes from the approach of TEM, SAXS and AFM measurements

Sakai, Kenichi; Tomizawa, H.; Tsuchiya, Koji; Ishida, Naoyuki; Sakai, Hideki; Abe, Masahiko

doi:10.1016/j.colsurfb.2008.07.017

Cited by 17 publications

(11 citation statements)

References 21 publications

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“…This effect on main transition endotherm may be explained as a result of mixing of PS with DPPC. This is in agreement with other studies investigating the similar structured stearylamine interacting with DPPC (Sakaia et al, 2008). In other words, the higher transition temperature observed from the compositions of DPPC and PS may indicate closer packing of the DPPC and PS molecules forming the lamellar bilayers.…”

Section: Resultssupporting

confidence: 91%

Analysis of skin penetration of phytosphingosine by fluorescence detection and influence of the thermotropic behaviour of DPPC liposomes

Hasanovic

Hoeller

Valenta

2010

International Journal of Pharmaceutics

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Section: Resultssupporting

confidence: 91%

Analysis of skin penetration of phytosphingosine by fluorescence detection and influence of the thermotropic behaviour of DPPC liposomes

Hasanovic

Hoeller

Valenta

2010

International Journal of Pharmaceutics

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“…46,47 Even in the absence of such an interfacial effect, hydrophobic/amphiphilic compounds may affect phospholipid monolayers, exhibiting characteristic π−A profiles, as seen with resveratrol 48 or 7-ethyl-10-hydroxy-campthothecin. 49 A similar effect was thus investigated for β-Lap at 3.5 mol % mixed with various lipids classically used in liposome preparation, namely, POPC, 5 0 , 5 1 DOPC, 5 2 , 5 3 and DPPC, 44,54,55 with or without cholesterol. CHOL usually strengthens the interactions between individual phospholipid molecules forming a membrane, ordering them and condensing a lipid layer.…”

Section: ■ Results and Discussionmentioning

confidence: 94%

“…Even in the absence of such an interfacial effect, hydrophobic/amphiphilic compounds may affect phospholipid monolayers, exhibiting characteristic π– A profiles, as seen with resveratrol or 7-ethyl-10-hydroxy-campthothecin . A similar effect was thus investigated for β-Lap at 3.5 mol % mixed with various lipids classically used in liposome preparation, namely, POPC, , DOPC, , and DPPC, ,, with or without cholesterol. CHOL usually strengthens the interactions between individual phospholipid molecules forming a membrane, ordering them and condensing a lipid layer. , The concentration of β-Lap in mixtures with lipids (3.5 mol %) was chosen after drug assay in LUV pellets, which showed that although 10 mol % drug was initially added to the phospholipid solution, the final β-Lap ER% varied between 2.7 and 3.4 ± 1.0 mol % only, depending on the nature of the phospholipid.…”

Section: Resultsmentioning

confidence: 99%

Deciphering the Peculiar Behavior of β-Lapachone in Lipid Monolayers and Bilayers

et al. 2019

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β-Lapachone (β-Lap) is a promising anticancer drug whose applications have been limited so far because of its poor solubility and stability. Its encapsulation in liposomes has been proposed to overcome these issues. However, surface pressure measurements show that β-Lap exhibits atypical interfacial behavior when mixed with lipids. Although the drug does not seem to be retained in lipid monolayers as deduced from the π−A isotherms, small changes in compressibility moduli suggest that β-Lap actually interacts with lipids, either disorganizing or rigidifying their monolayers. Thermal and structural analyses of lipid bilayers confirm the existence of β-Lap/ lipid interactions and show that the drug inserts between hydrophobic chains, close to the polar headgroup in DPPC bilayers and deeper in the acyl chains in POPC bilayers. Molecular dynamics simulations allow a comprehensive description of the drug position and orientation in DOPC and POPC bilayers in the presence or absence of cholesterol.

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“…The choice of DPPC resides in the enormous amount of studies available in the literature on the structure and properties of bilayers formed by this lipid. DPPC has often been used as a model system to study the interactions of drugs with eukaryotic membranes . We should mention that the gel-to-fluid transition of DPPC bilayers at full hydration takes place at 42 °C, which is 5 °C above the body temperature of a healthy human being.…”

Section: Introductionmentioning

confidence: 99%

“…DPPC has often been used as a model system to study the interactions of drugs with eukaryotic membranes. 15 We should mention that the gelto-fluid transition of DPPC bilayers at full hydration takes place at 42 °C, which is 5 °C above the body temperature of a healthy human being. Even if this feature could appear to suggest the unsuitability of this lipid for studies with a biologic target, DPPC is commonly used for structural studies because the effect of the inclusion of other molecular species in the bilayers is easily monitored by means of calorimetric methods.…”

Section: ■ Introductionmentioning

confidence: 99%

Structural Modifications of DPPC Bilayers upon Inclusion of an Antibacterial Cationic Bolaamphiphile

2018

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The emergence of antibiotic-resistant bacterial strains has fostered fundamental research to develop alternative antimicrobial strategies. Among the several systems proposed so far, the association complexes (nanoplexes) formed by transcription factor decoys (TFDs), i.e., short oligonucleotides targeting a crucial bacterial transcription factor, and a bolaform cationic amphiphile, 10,10'-(dodecane-1,12-diyl)-bis-(9-amino-1,2,3,4-tetrahydroacridinium) chloride (12-bis-THA), have demonstrated their potential in vitro and in vivo. The application of these nanoplexes is hampered by a scarce colloidal stability, which can be addressed by including the bolaamphiphile in a liposomal carrier, which is then associated to the TFD. The present study reports an investigation on the effects of 12-bis-THA on the structure of synthetic lipid bilayers to assess the morphology of the mixed assemblies, gain insight into the location of the host within the bilayer, and determine the loading capacity of the carrier. Our results demonstrate that 12-bis-THA promptly inserts within 1,2-dipalmitoyl- sn-glycero-3-phosphocholine (DPPC) bilayers, bending its C-12 spacer chain to adopt a conelike shape and shifting the gel-liquid crystalline transition of the chains to lower temperatures. The host liposomal structure is retained for a bolaamphiphile concentration of up to 3.2% mol to DPPC, whereas higher concentrations lead to the destabilization by means of a detergency-like mechanism, with the simultaneous existence of different lamellar-based structures, such as liposomes, bicelles, and rafts, in which DPPC and 12-bis-THA could be present in different molar ratios. Overall, these results shed light on the interaction of the bolaamphiphile with a lipid bilayer and provide valuable insight to better formulate the antimicrobial amphiphile in liposomal carriers to circumvent the colloidal instability of nanoplexes.

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Characterizing the structural transition of cationic DPPC liposomes from the approach of TEM, SAXS and AFM measurements

Cited by 17 publications

References 21 publications

Analysis of skin penetration of phytosphingosine by fluorescence detection and influence of the thermotropic behaviour of DPPC liposomes

Analysis of skin penetration of phytosphingosine by fluorescence detection and influence of the thermotropic behaviour of DPPC liposomes

Deciphering the Peculiar Behavior of β-Lapachone in Lipid Monolayers and Bilayers

Structural Modifications of DPPC Bilayers upon Inclusion of an Antibacterial Cationic Bolaamphiphile

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