Characterizing the NO3 and NH4 Uptake Process of Rice Roots by Use of 15N Labelled NH4NO3

Fried, Maurice; Zsoldos, Ferenc; Vose, P. B.; Shatokhin, I. L.

doi:10.1111/j.1399-3054.1965.tb06894.x

Cited by 102 publications

(43 citation statements)

References 10 publications

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“…For optimal grain yield, paddy rice farming requires extensive watering (flooded by rivers and rainfall during the monsoon season, or by irrigation) for three quarters of the growing season, which influences the availability of the macronutrient nitrogen (N) in the soil. In non-aerated paddy fields, rice mobilizes ammonium (NH 4 + ) rather than nitrate (NO 3 − ) as the preferred N source (Fried et al 1965;Sasakawa and Yamamoto 1978). Yet, plant growth, yield and N-use efficiency are significantly enhanced when NH 4 + and NO 3 − are provided simultaneously (Kronzucker et al 1999;Kronzucker et al 2000;Duan et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Drechsler¹,

Courty²,

Brulé³

et al. 2017

Mycorrhiza

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Arbuscular mycorrhizal fungi (AMF) colonize up to 90% of all land plants and facilitate the acquisition of mineral nutrients by their hosts. Inorganic orthophosphate (P i ) and nitrogen (N) are the major nutrients transferred from the fungi to plants. While plant P i transporters involved in nutrient transfer at the plant-fungal interface have been well studied, the plant N transporters participating in this process are largely unknown except for some ammonium transporters (AMT) specifically assigned to arbuscule-colonized cortical cells. In plants, many nitrate transporter 1/peptide transporter family (NPF) members are involved in the translocation of nitrogenous compounds including nitrate, amino acids, peptides and plant hormones. Whether NPF members respond to AMF colonization, however, is not yet known. Here, we investigated the transcriptional regulation of 82 rice (Oryza sativa) NPF genes in response to colonization by the AMF Rhizophagus irregularis in roots of plants grown under five different nutrition regimes. Expression of the four OsNPF genes NPF2.2/ PTR2, NPF1.3, NPF6.4 and NPF4.12 was strongly induced in mycorrhizal roots and depended on the composition of the fertilizer solution, nominating them as interesting candidates for nutrient signaling and exchange processes at the plantfungal interface.

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Section: Introductionmentioning

confidence: 99%

Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Drechsler¹,

Courty²,

Brulé³

et al. 2017

Mycorrhiza

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“…Rather, at best, N mixtures of varying proportions must be expected in soil solution. Although the uptake of NO $ − is inhibited by the presence of NH % + in many species by as much as 50 % (Glass & Siddiqi, 1995 ;Kronzucker et al, 1999b), with a similar extent of inhibition observed in rice (Fried et al, 1965 ;Colmer & Bloom, 1998 ;Kronzucker et al, 1999a), co-provision of NO $ − and NH % + has been shown to facilitate a significant enhancement of growth and yield (Cox & Reisenauer, 1973 ;Ta & Ohira, 1981 ;Ancheng et al, 1993 ;Kronzucker et al, 1999b) compared with provision of either N source alone. Increases as high as 40-70 % in controlled-culture conditions have been reported, and somewhat lower, but still appreciable, enhancements are seen in field conditions (Gill & Reisenauer, 1993).…”

Section: mentioning

confidence: 99%

“…In sharp contrast to most agricultural soils, where nitrate (NO $ − ) is the predominant N species (Kronzucker et al, 1998), hypoxic conditions in the paddy environment largely preclude the microbial formation of NO $ − through nitrification (Bouldin, 1986 ;Arth et al, 1998) and, consequently, ammonium (NH % + ) is the main form of N available to rice in the field (Shen, 1969 ;Wang et al, 1993 ;Arth et al, 1998 ;Kronzucker et al, 1998). It is therefore not surprising that NH % + nutrition, as opposed to NO $ − nutrition, has received almost exclusive attention in rice (Bonner, 1946 ;Fried et al, 1965 ;Shen, 1969 ;Wang et al, 1993). However, some reports have indicated that rice does possess some capacity for root NO $ − absorption (Ismunadji & Dijkshoorn, 1971 ;Sasakawa & Yamamoto, 1978 ;Youngdahl et al, 1982 ;Raman et al, 1995) and for the reduction of NO $ − in leaves (Tang & Wu, 1957).…”

Section: mentioning

confidence: 99%

Comparative kinetic analysis of ammonium and nitrate acquisition by tropical lowland rice: implications for rice cultivation and yield potential

et al. 2000

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Nitrogen limitation compromises the realization of yield potential in cereals more than any other single factor. In rice, the world's most important crop species, the assumption has long been that only ammonium-N is efficiently utilized. Consequently, nitrate utilization has been largely ignored, although fragmentary data have suggested that growth could be substantial on nitrate. Using the short-lived radiotracer "$N, we here provide direct comparisons of root transmembrane fluxes and cytoplasmic pool sizes for nitrate-and ammonium-N in a major variety of Indica rice (Oryza sativa), and show that nitrate acquisition is not only of high capacity and efficiency but is superior to that of ammonium. We believe our results have implications for rice breeding and molecular genetics as well as the design of water-management and fertilization regimes. Potential strategies to harness this hitherto unexplored N-utilization potential are proposed.

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“…Although ammonium concentrations are often 10 to 1,000 times lower than those of nitrate in well-aerated soil, ammonium nutrition plays an essential role in waterlogged and acid soils (Marschner, 1995). Furthermore, ammonium seems to be a preferred source of N and is taken up more rapidly than nitrate when both ions are presented simultaneously to plants (Gazzarrini et al, 1999).Physiological studies of ammonium transport into roots have revealed biphasic kinetics in several species (Fried et al, 1965;Vale et al, 1988;Wang et al, 1993). The so-called high-affinity ammonium transport system is predominant at low (submillimolar) concentrations of substrate (NH 4 ϩ ) and exhibits saturation kinetics.…”

mentioning

confidence: 99%

Characterization of Arabidopsis AtAMT2, a High-Affinity Ammonium Transporter of the Plasma Membrane

et al. 2002

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AtAMT2 is an ammonium transporter that is only distantly related to the five members of the AtAMT1 family of high-affinity ammonium transporters in Arabidopsis. The short-lived radioactive ion 13 NH 4 ϩ was used to show that AtAMT2, expressed in yeast (Saccharomyces cerevisiae), is a high-affinity transporter with a K m for ammonium of about 20 m. Changes in external pH between 5.0 and 7.5 had little effect on the K m for ammonium, indicating that NH 4 ϩ , not NH 3 , is the substrate for AtAMT2. The AtAMT2 gene was expressed in all organs of Arabidopsis and was subject to nitrogen (N) regulation, at least in roots where expression was partially repressed by high concentrations of ammonium nitrate and derepressed in the absence of external N. Although expression of AtAMT2 in shoots responded little to changes in root N status, transcript levels in leaves declined under high CO 2 conditions. Transient expression of an AtAMT2-green fluorescent protein fusion protein in Arabidopsis leaf epidermal cells indicated a plasma membrane location for the AtAMT2 protein.Thus, AtAMT2 is likely to play a significant role in moving ammonium between the apoplast and symplast of cells throughout the plant. However, a dramatic reduction in the level of AtAMT2 transcript brought about by dsRNA interference with gene expression had no obvious effect on plant growth or development, under the conditions tested.Ammonium and nitrate are thought to be the primary sources of nitrogen (N) for most plants growing in agricultural soils. Acquisition of these inorganic nutrients from the soil solution involves a variety of different transporters, which transport the ions from the apoplast of root epidermal and cortical cells into the symplast. Although ammonium concentrations are often 10 to 1,000 times lower than those of nitrate in well-aerated soil, ammonium nutrition plays an essential role in waterlogged and acid soils (Marschner, 1995). Furthermore, ammonium seems to be a preferred source of N and is taken up more rapidly than nitrate when both ions are presented simultaneously to plants (Gazzarrini et al., 1999).Physiological studies of ammonium transport into roots have revealed biphasic kinetics in several species (Fried et al., 1965;Vale et al., 1988;Wang et al., 1993). The so-called high-affinity ammonium transport system is predominant at low (submillimolar) concentrations of substrate (NH 4 ϩ ) and exhibits saturation kinetics. A second component of ammonium uptake is the low-affinity transport system, which becomes significant at higher external ammonium concentrations (above 1 mm) and exhibits nonsaturation kinetics (Wang et al., 1993;Kronzucker et al., 1996). Although the molecular basis of low-affinity transport system activity remains unknown, there is growing evidence that members of the AMT1 family of transporters are responsible for high-affinity ammonium transport system activity in plants. The first AMT1 gene to be discovered in plants was AtAMT1;1 from Arabidopsis, which was cloned by complementation of a yeast (Sacchar...

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Characterizing the NO₃ and NH₄ Uptake Process of Rice Roots by Use of ¹⁵N Labelled NH₄NO₃

Cited by 102 publications

References 10 publications

Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Identification of arbuscular mycorrhiza-inducible Nitrate Transporter 1/Peptide Transporter Family (NPF) genes in rice

Comparative kinetic analysis of ammonium and nitrate acquisition by tropical lowland rice: implications for rice cultivation and yield potential

Characterization of Arabidopsis AtAMT2, a High-Affinity Ammonium Transporter of the Plasma Membrane

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