Characterizing the Mechanism of Serotonin Alleviates Rice Resistance to Brown Planthopper Nilaparvata lugens (Homoptera: Delphacidae) Nymphs

Abstract

Serotonin is a well-known secondary metabolite that plays an important role in many growth and developmental processes, as well as biotic and abiotic stress responses of plants. Yet, whether serotonin biosynthesis regulates the resistance of rice to the brown planthopper (BPH) Nilaparvata lugens and its underling mechanisms have not been entirely investigated. In this study, we found that expression levels of four serotonin biosynthesis gene, OsTDC1, OsTDC2, OsTDC3, and OsT5H, and the serotonin content were significantly induced by BPH nymph infestation. The rice seedlings pretreated with serotonin were more attractive to BPH nymphs and promoted their feeding behavior as indicated by increased honeydew excretion. In addition, serotonin application to artificial diets increased the survival rates of BPH nymphs in a dose-dependent manner compared to the controls. Moreover, the exogenous serotonin application to rice seedlings increased the average injury scale and functional plant loss indices caused by BPH nymph infestation. Moreover, supplemented serotonin enhanced soluble sugar and free amino acid contents, as well as the activities of SOD, POD, and PPO, but repressed the production of flavonoids. Our findings illustrated that serotonin plays a negative role in the regulation of rice resistance to BPH nymphs probably by modulating contents of soluble sugars, free amino acids, and flavonoids.

Effect of Paclobutrazol Application on Enhancing the Efficacy of Nitenpyram against the Brown Planthopper, Nilaparvata lugens

“…In rice, phenolics and flavonoids are important secondary metabolites related to BPH resistance. Reducing the flavonoid level of rice can decrease the resistance of rice to brown planthoppers, resulting in an increase in the survival rate of brown planthoppers, while improving flavonoid levels leads to the opposite result [ 23 , 24 ]. The decrease in phenolic content also leads to the lower resistance of rice to brown planthoppers [ 25 ].…”