Characterizing the lethal and sub‐lethal effects of genetically modified soybean expressing <scp>Cry1A</scp>.105, <scp>Cry2Ab2</scp>, and <scp>Cry1Ac</scp> insecticidal proteins against <i>Spodoptera</i> species (Lepidoptera: Noctuidae) in Brazil

Barcellos, Giovani A.; Hanich, Manoela Ramos; Pretto, Venicius E.; Weschenfelder, Marlon A. G.; Horikoshi, Renato J.; Dourado, Patrick M.; Ovejero, R. F. Lopez; Berger, G.; Martinelli, Samuel; Head, Graham P.; Bernardi, Oderlei

doi:10.1002/ps.7225

Cited by 10 publications

(12 citation statements)

References 30 publications

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“…These parameters coincide with studies where other lepidopterans experienced alterations in their developmental parameters when ingesting Bt proteins (Pérez-Hedo et al 2011,Barcellos et al 2023). Sublethal doses of Bt can induce an increase in the weight of the larvae, as was observed in the Rnu-SE population, coinciding with studies carried out on Spodoptera eridania (Stoll), there was an effect of hormesis at lower doses of the Cry1Ac toxin(Rabelo et al 2020).…”

supporting

confidence: 85%

Field-evolved resistance of Rachiplusia nu to Cry1Ac Bt soybean in Argentina

Hill,

Jerez,

Linares

et al. 2023

Preprint

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Large-scale adoption of genetically engineered soybeans expressing insecticidal proteins from Bacillus thuringiensis (Bt) in South America allow us to understand the ability of pest populations to evolve resistance to Bt crops. Here, we examined the susceptibility of Rachiplusia nu (Guenée) and Chrysodeixis includens (Walker) (Noctuidae: Plusiinae) collected from Cry1Ac Bt and non-Bt soybean fields in Argentina, specifically in the Provinces of Santiago del Estero (Rnu-SE), Córdoba (Rnu-COR), and Tucumán (Cin-TUC), to Cry1Ac toxin. In Cry1Ac-overlay bioassays, the lethal concentration to kill 50% of the larvae (LC50) of Rnu-COR and Cin-TUC populations were 113 and 1,500 ng/cm2, respectively. The lower limit of the LC50 value for Rnu-SE larvae was > 21,679 ng/cm2, at least 14-fold the LC50 value for Cin-TUC larvae and at least 192-fold the LC50 value for Rnu-COR larvae. A similar pattern emerged from the effective Cry1Ac concentrations that inhibited 50% of larval growth. In larval feeding assays using Bt soybean leaves, Rnu-SE insects had higher survival rates than Rnu-COR and Cin-TUC ones. In behavioral assays with Bt and non-Bt soybean plants, the R. nu moths showed a strong oviposition preference for the Cry1Ac Bt over non-Bt soybean plants. These results indicate that a population of R. nu evolved Cry1Ac resistance in Argentina, consistent with the increased number of plusiine loopers causing unexpected damage in Cry1Ac Bt soybean fields. That may correlate with moth oviposition behavior, and further research should guide locally adapted strategies to minimize the resistance drawbacks and maximize the benefits of Bt crops.

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supporting

confidence: 85%

Field-evolved resistance of Rachiplusia nu to Cry1Ac Bt soybean in Argentina

Hill,

Jerez,

Linares

et al. 2023

Preprint

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“…This genetically modified (GM) soybean confers protection against Anticarsia gemmatalis (Hübner, 1818) (Lepidoptera: Erebidae) and against Chrysodeixis includens (Walker, [1858]), Chloridea virescens (Fabricius, 1781) and Helicoverpa armigera (Hübner, 1808) (Lepidoptera: Noctuidae) [ 4 , 5 , 6 , 7 , 8 ] and is cultivated on more than 36 million hectares per season in Brazil [ 9 ]. During the 2021/2022 season, the event MON 87751 × MON 87708 × MON 87701 × MON 89788 (trade name Intacta 2 Xtend ® ), expressing Cry1A.105, Cry2Ab2 and Cry1Ac proteins, was also released for commercial use in Brazil, extending the protection conferred by Bt soybean to the species S. cosmioides [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previous reports have stated that Cry1A.105/Cry2Ab2/Cry1Ac and Cry1Ac soybean technologies had reduced lethality against S. eridania and S. frugiperda [ 5 , 8 , 10 ]. This can be explained by the naturally low susceptibility of these species to Bt proteins [ 5 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Dose Effects of Flubendiamide and Thiodicarb against Spodoptera Species Developing on Bt and Non-Bt Soybean

Godoy,

Pretto,

de Almeida

et al. 2023

Insects

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An increase in Spodoptera species was reported in Bt soybean fields expressing Cry1Ac insecticidal proteins in Brazil, requiring additional management with chemical insecticides. Here, we evaluated the dose effects of flubendiamide and thiodicarb on Spodoptera cosmioides (Walker, 1858), Spodoptera eridania (Stoll, 1782), Spodoptera albula (Walker, 1857) and Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) that survived on MON 87751 × MON 87708 × MON 87701 × MON 89788, expressing Cry1A.105, Cry2Ab2 and Cry1Ac; MON 87701 × MON 89788 soybean, expressing Cry1Ac; and non-Bt soybean. On unsprayed Cry1A.105/Cry2Ab2/Cry1Ac soybean, only S. frugiperda showed ~60% mortality after 10 d, whereas S. cosmioides, S. eridania and S. albula showed >81% mortality. The surviving larvae of all species on this Bt soybean showed >80% mortality when exposed to the field label dose of flubendiamide (70 mL/ha) or thiodicarb (400 g/ha) or at 50% of these doses. In contrast, all four species had <25% and <19% mortality on Cry1Ac and non-Bt soybean, respectively. The surviving S. cosmioides, S. eridania and S. albula on these soybean types presented >83% mortality after exposure to both dose levels of flubendiamide and thiodicarb. Some S. frugiperda larvae surviving on Cry1Ac and non-Bt soybean sprayed with a 50% dose of either insecticide developed into adults. However, the L1 larvae developing on Cry1Ac soybean leaves sprayed with flubendiamide and the L2 larvae on this soybean sprayed with thiodicarb had a prolonged immature stage, and the females displayed lower fecundity, which are likely to impact S. frugiperda population growth on soybean.

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“…Except for S. frugiperda, no larvae of the tested Spodoptera species developed into adults on this soybean type. Results from previous studies also showed that no larvae of S. cosmioides, S. albula or S. eridania were able to develop to the adult stage on Cry1A.105/Cry2Ab2/Cry1Ac soybean [10]. Nonetheless, none of the L1 and L2 larvae of Spodoptera species surviving on Cry1A.105/Cry2Ab2/Cry1Ac soybean for 10 d (∼40% for S. frugiperda and <20% for other species tested) survived until the adult stage when exposed to leaves sprayed with flubendiamide or thiodicarb at the field label dose or 50% of this dose.…”

Section: Discussionmentioning

confidence: 86%

Dose Effects of Flubendiamide and Thiodicarb Against Spodoptera Species Developing on Bt and Non-Bt Soybean

Godoy,

Pretto,

Almeida

et al. 2023

Preprint

View full text Add to dashboard Cite

An increase in Spodoptera species was reported in Bt soybean fields expressing Cry1Ac insecticidal proteins in Brazil, requiring additional management with chemical insecticides. Here, we evaluated the dose effects of flubendiamide and thiodicarb on Spodoptera cosmioides (Walker, 1858), Spodoptera eridania (Stoll, 1782), Spodoptera albula (Walker, 1857) and Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) that survived on MON 87751 × MON 87708 × MON 87701 × MON 89788, expressing Cry1A.105, Cry2Ab2 and Cry1Ac; MON 87701 × MON 89788 soybean, expressing Cry1Ac; and non-Bt soybean. On unsprayed Cry1A.105/Cry2Ab2/Cry1Ac soybean, only S. frugiperda showed ~60% mortality after 10 d, whereas S. cosmioides, S. eridania and S. albula showed >81% mortality. Surviving larvae of all species on this Bt soybean showed &gt;80% mortality when exposed to the field label dose of flubendiamide (70 mL/ha) or thiodicarb (400 g/ha) or at 50% of these doses. In contrast, all four species had <25% and <19% mortality on Cry1Ac and non-Bt soybean, respectively. Surviving S. cosmioides, S. eridania and S. albula on these soybean types presented >83% mortality post-exposure to both dose levels of flubendiamide and thiodicarb. Some S. frugiperda larvae surviving on Cry1Ac and non-Bt soybean sprayed with a 50% dose of either insecticide developed into adults. However, L1 larvae developing on Cry1Ac soybean leaves sprayed with flubendiamide and L2 larvae on this soybean sprayed with thiodicarb had a prolonged immature stage, and females lower fecundity, which are likely to impact its population growth on soybean.

show abstract

Characterizing the lethal and sub‐lethal effects of genetically modified soybean expressing Cry1A.105, Cry2Ab2, and Cry1Ac insecticidal proteins against Spodoptera species (Lepidoptera: Noctuidae) in Brazil

Cited by 10 publications

References 30 publications

Field-evolved resistance of Rachiplusia nu to Cry1Ac Bt soybean in Argentina

Field-evolved resistance of Rachiplusia nu to Cry1Ac Bt soybean in Argentina

Dose Effects of Flubendiamide and Thiodicarb against Spodoptera Species Developing on Bt and Non-Bt Soybean

Dose Effects of Flubendiamide and Thiodicarb Against Spodoptera Species Developing on Bt and Non-Bt Soybean

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