Characterizing the impact of Enterococcus cecorum infection during late embryogenesis on disease progression, cecal microbiome composition, and early performance in broiler chickens

Arango, Marcela; Forga, Aaron; Liu, Jing; Zhang, Guolong; Gray, Latasha; Moore, Randy; Coles, Makenly; Atencio, Abdiel; Trujillo, Carolina; Latorre, Juan David; Tellez-Isaias, Guillermo; Hargis, Billy; Graham, Danielle

doi:10.1016/j.psj.2023.103059

Cited by 3 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The virulence testing of the isolates is also problematic. Some have relied on chicken embryo lethality assays for the evaluation of E.cecorum isolates [ 17 , 48 , 49 ], but the direct relevance to pathogenicity post-hatch has been a concern [ 50 ]. Experimental infections of young chicks with isolates of E. cecorum have shown some promise [ 10 ] but have not been used to evaluate the relative virulence of different isolates.…”

Section: Discussionmentioning

confidence: 99%

“…The recent occurrence of sepsis outbreaks in broilers caused by E. cecorum is characterized by onset during the first few weeks post-hatch, prior to the normal colonization of the intestinal tract [ 17 ]. However, there are older reports of sepsis in broilers caused by E. cecorum , with losses of 5–8%, characterized by pericarditis, bacteremia, and osteomyelitis [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, there are older reports of sepsis in broilers caused by E. cecorum , with losses of 5–8%, characterized by pericarditis, bacteremia, and osteomyelitis [ 18 ]. The mechanisms of the horizontal or vertical spread of E. cecorum in broiler flocks are not known, although particular facilities or flocks may experience repetitive outbreaks [ 17 , 18 ]. Recent outbreaks may be more problematic with the removal of early administration of antibiotic growth promoters in flocks [ 17 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…The mechanisms of the horizontal or vertical spread of E. cecorum in broiler flocks are not known, although particular facilities or flocks may experience repetitive outbreaks [ 17 , 18 ]. Recent outbreaks may be more problematic with the removal of early administration of antibiotic growth promoters in flocks [ 17 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…Phylogenomic analysis of more than 100 genomes of E. cecorum isolates from French broilers suggested that a specific lineage of pathogenic isolates is infecting broilers in Europe and the United States [16]. The recent occurrence of sepsis outbreaks in broilers caused by E. cecorum is characterized by onset during the first few weeks post-hatch, prior to the normal colonization of the intestinal tract [17]. However, there are older reports of sepsis in broilers caused by E. cecorum, with losses of 5-8%, characterized by pericarditis, bacteremia, and osteomyelitis [18].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Molecular Genomic Analyses of Enterococcus cecorum from Sepsis Outbreaks in Broilers

Rhoads,

Pummill,

Alrubaye

2024

Microorganisms

View full text Add to dashboard Cite

Extensive genomic analyses of Enterococcus cecorum isolates from sepsis outbreaks in broilers suggest a polyphyletic origin, likely arising from core genome mutations rather than gene acquisition. This species is a normal intestinal flora of avian species with particular isolates associated with osteomyelitis. More recently, this species has been associated with sepsis outbreaks affecting broilers during the first 3 weeks post-hatch. Understanding the genetic and management basis of this new phenotype is critical for developing strategies to mitigate this emerging problem. Phylogenomic analyses of 227 genomes suggest that sepsis isolates are polyphyletic and closely related to both commensal and osteomyelitis isolate genomes. Pangenome analyses detect no gene acquisitions that distinguish all the sepsis isolates. Core genome single nucleotide polymorphism analyses have identified a number of mutations, affecting the protein-coding sequences, that are enriched in sepsis isolates. The analysis of the protein substitutions supports the mutational origins of sepsis isolates.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular Genomic Analyses of Enterococcus cecorum from Sepsis Outbreaks in Broilers

Rhoads,

Pummill,

Alrubaye

2024

Microorganisms

View full text Add to dashboard Cite

show abstract

A rare case of invasive Enterococcus cecorum infection and related diagnostic difficulties

Szymanski,

Skiba,

Piasecka

et al. 2024

Clinical Case Reports

View full text Add to dashboard Cite

Key Clinical MessageThis report presents a rare case of invasive infection caused by Enterococcus cecorum. There are no specific guidelines regarding antibiotic therapy for this infection. Based on this case, it can be concluded that linezolid demonstrates in vivo activity against Enterococcus cecorum and can be successfully used in therapy.AbstractEnterococcus cecorum is an extremely rare pathogen in humans. Since 1984, when the microorganism was first described, only a dozen cases of invasive infections in humans have been reported in the literature. The diagnostic pathway may involve difficulties in correctly identifying this microorganism. Based on the case described, it can be thought that Enterococcus cecorum is a more challenging bacterium than the much more common Enterococcus faecium or Enterococcus faecalis. The described case underscores the importance of medical vigilance in clinical practice. It seems that due to increasingly advanced techniques in molecular biology, we will more frequently detect pathogens that were previously encountered only sporadically. Since not every center has access to modern and advanced microbiological diagnostic methods, publications that practically combine classical microbiological diagnostic methods with those less accessible but more modern are exceptionally valuable. In the case described, it is also worth noting that classical methods still play a significant and crucial role in conducting microbiological diagnostics. In the era of rapid diagnostic tool development, it is important to emphasize the necessity of combining different methods rather than replacing one with another.

show abstract

Molecular genomic analyses of Enterococcus cecorum from sepsis outbreaks in broilers

Rhoads,

Pummill,

Alrubaye

2024

Preprint

View full text Add to dashboard Cite

Extensive genomic analyses of Enterococcus cecorum isolates from sepsis outbreaks in broilers suggests a polyphyletic origin likely arising from core genome mutations rather than gene acquisition. This species is normal intestinal flora in avian species with particular isolates associated with osteomyelitis. More recently, the species has been associated with sepsis outbreaks affecting broilers during the first 3 weeks post hatch. Understanding the genetic and management basis of this new phenotype is critical for developing strategies to mitigate this emerging problem. Phylogenomic analyses of 227 genomes suggest that sepsis isolates are polyphyletic and closely related to both commensal and osteomyelitis isolate genomes. Pangenome analyses detect no gene acquisitions that distinguish all sepsis isolates. Core genome SNP analyses identify a number of mutations, affecting protein coding sequence, that are enriched in sepsis isolates. Analysis of the protein substitutions supports mutational origins of sepsis isolates.

show abstract

Characterizing the impact of Enterococcus cecorum infection during late embryogenesis on disease progression, cecal microbiome composition, and early performance in broiler chickens

Cited by 3 publications

References 28 publications

Molecular Genomic Analyses of Enterococcus cecorum from Sepsis Outbreaks in Broilers

Molecular Genomic Analyses of Enterococcus cecorum from Sepsis Outbreaks in Broilers

A rare case of invasive Enterococcus cecorum infection and related diagnostic difficulties

Molecular genomic analyses of Enterococcus cecorum from sepsis outbreaks in broilers

Contact Info

Product

Resources

About