Characterizing the effect of Poast on Chlorella vulgaris, a non-target organism

Smythers, Amanda L.; Garmany, Armin; Perry, Nicole L.; Higginbotham, Ethan; Adkins, Ethan; Kolling, Derrick R.J.

doi:10.1016/j.chemosphere.2018.12.050

Cited by 8 publications

(22 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metolachlor was tested at 100, 200, and 300 µg l −1 , according to the toxicity level in microalgae listed in King et al (2017). Sethoxydim was tested at concentrations 0.05, 0.08, and 0.1% v/v, following the study of Smythers et al (2019). Each herbicide tolerance test was conducted for 1 week, on a total volume of 10 ml in sterile glass tubes (30 ml capacity), under the same growth conditions in which microalgae cultures were kept during the previous months/years.…”

Section: Herbicidesmentioning

confidence: 99%

Isolation of Four Microalgal Strains From the Lake Massaciuccoli: Screening of Common Pollutants Tolerance Pattern and Perspectives for Their Use in Biotechnological Applications

et al. 2020

View full text Add to dashboard Cite

Aquatic ecosystems represent one of the largest reservoirs of phytoplankton accounting for most of the primary production of the Earth. The Lake Massaciuccoli located in Tuscany (Italy) is one of the largest swamps that in ancient times entirely covered the Versilia coastal plain. Despite its peculiar features, especially the eutrophic characteristics, its native microalgal consortia have never been explored up to now. In this work, we isolated and described four autochthonous microalgal strains from different sites in the lake (FB, Idr, CL_Sc, and CL_Ch); the four microalgal strains were identified within the Chlorella sorokiniana clade. We exposed them to ten of the most common or emerging environmental contaminants in order to describe their preliminary response to the tested substances: five metals (As, Fe, Ni, Cu, and Zn), two herbicides (Metolachlor and Sethoxydim), two antibiotics (Ciprofloxacin and Benzylpenicillin) and a non-steroidal anti-inflammatory drug (Ibuprofen). Physiological response of the strains highlighted intraspecific differences; strain CL_Sc was the most tolerant in presence of metals while strain Idr was the most sensitive. All strains were sensitive to sethoxydim and tolerant to metolachlor at all the tested concentrations. Strains FB and Idr were the most sensitive in presence of Ibuprofen while strain CL_Ch was the most sensitive to the highest Benzylpenicillin concentration. Resistance pattern of strain Idr somehow reflects both the phylogenetic and the geographic “isolation” from all other three strains. Finally, optical microscope observation confirmed some differences also in the microalgae morphological aspect. Overall, all the strains showed interesting responses in presence of high concentrations of the tested substances, representing putative interesting candidates for water remediation in wastewater treatment plants.

show abstract

Section: Herbicidesmentioning

confidence: 99%

Isolation of Four Microalgal Strains From the Lake Massaciuccoli: Screening of Common Pollutants Tolerance Pattern and Perspectives for Their Use in Biotechnological Applications

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Spectroscopic cell density (turbidity) was measured using a Shimadzu UV-1800 spectrophotometer (Shimadzu Corp., Kyoto, Japan) at 750 nm as previously described [37,38]. Cell diameter was determined using a micrometer slide on a VistaVision light microscope (VWR International, at 1000× magnification.…”

Section: Methodsmentioning

confidence: 99%

“…Dry mass was measured as previously reported [38]. Briefly, 1 mL of cells were pelleted and rinsed with H 2 O and filtered onto pre-weighed 1 μm, 25 mm GF/B Whatman glass microfiber filters (Whatman International Ltd., Maidstone, UK) using a Büchner funnel.…”

Section: Methodsmentioning

confidence: 99%

“…Terminal carbohydrates were assayed as previously described using the acid-phenol assay [38,43]. Briefly, 100 µL of sample was collected in triplicate from each culture and pelleted, discarding the supernatant.…”

Section: Methodsmentioning

confidence: 99%

“…Photosynthetic electron transfer fluxes were inferred from Chl a fluorescence using a Photon Systems Instruments FL 3500 fluorometer (Drasov, Czech Republic) as previously described [38]. The OJIP protocol included a 1s actinic illumination using a 630 nm light at an intensity of 2400 µmole photons · m −2 · s −1 .…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Inhibition of TOR in Chlamydomonas reinhardtii Leads to Rapid Cysteine Oxidation Reflecting Sustained Physiological Changes

Ford

Smythers

McConnell

et al. 2019

Cells

Self Cite

View full text Add to dashboard Cite

The target of rapamycin (TOR) kinase is a master metabolic regulator with roles in nutritional sensing, protein translation, and autophagy. In Chlamydomonas reinhardtii, a unicellular green alga, TOR has been linked to the regulation of increased triacylglycerol (TAG) accumulation, suggesting that TOR or a downstream target(s) is responsible for the elusive “lipid switch” in control of increasing TAG accumulation under nutrient limitation. However, while TOR has been well characterized in mammalian systems, it is still poorly understood in photosynthetic systems, and little work has been done to show the role of oxidative signaling in TOR regulation. In this study, the TOR inhibitor AZD8055 was used to relate reversible thiol oxidation to the physiological changes seen under TOR inhibition, including increased TAG content. Using oxidized cysteine resin-assisted capture enrichment coupled with label-free quantitative proteomics, 401 proteins were determined to have significant changes in oxidation following TOR inhibition. These oxidative changes mirrored characterized physiological modifications, supporting the role of reversible thiol oxidation in TOR regulation of TAG production, protein translation, carbohydrate catabolism, and photosynthesis through the use of reversible thiol oxidation. The delineation of redox-controlled proteins under TOR inhibition provides a framework for further characterization of the TOR pathway in photosynthetic eukaryotes.

show abstract

Chlorella vulgaris bioaccumulates excess manganese up to 55× under photomixotrophic conditions

2019

View full text Add to dashboard Cite

Characterizing the effect of Poast on Chlorella vulgaris, a non-target organism

Cited by 8 publications

References 36 publications

Isolation of Four Microalgal Strains From the Lake Massaciuccoli: Screening of Common Pollutants Tolerance Pattern and Perspectives for Their Use in Biotechnological Applications

Isolation of Four Microalgal Strains From the Lake Massaciuccoli: Screening of Common Pollutants Tolerance Pattern and Perspectives for Their Use in Biotechnological Applications

Inhibition of TOR in Chlamydomonas reinhardtii Leads to Rapid Cysteine Oxidation Reflecting Sustained Physiological Changes

Chlorella vulgaris bioaccumulates excess manganese up to 55× under photomixotrophic conditions

Contact Info

Product

Resources

About