Characterizing the composition of intestinal microflora as a prospective treatment target in infant allergic disease

Kirjavainen, Pirkka V.; Apostolou, Effie; Arvola, Taina; Salminen, Seppo; Gibson, Glenn R.; Isolauri, Erika

doi:10.1111/j.1574-695x.2001.tb00526.x

Cited by 151 publications

(76 citation statements)

References 47 publications

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“…Various results of the diversity in the Lactobacillus and Bifidobacterium groups in the allergy patients have been reported; on the one hand, the prevalence of colonic Bifidobacteria was lower in allergic infants than in healthy infants (10,28,35). In addition, Thompson-Chagoyan et al (4) found no differences between children with CMPA and healthy children in terms of the prevalence of the Bifidobacterium and Lactobacillus groups in gut flora.…”

Section: Discussionmentioning

confidence: 95%

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Comparative Analysis of Fecal Microbiota in 5 - 8-Year-old Children with and without Cow Milk Protein Allergy

et al. 2016

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Background: The bacterial diversity and microbial communities in the feces of children with cow milk protein allergy (CMPA) may be altered; these changes have not been clearly elucidated in 5 -8-year-old children with CMPA. Objectives: The aim of this study was to compare the bacterial diversity and microbial communities of feces in 5 -8-year-old children with CMPA and those of healthy children of the same age. Methods: Stool samples from 12 children with CMPA and 12 healthy individuals were collected. Total bacterial DNA of all samples were extracted and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) using bacterial v3 universal primers and the species-specific primers of Bifidobacterium, Lactobacillus, and Clostridium coccoides. The preponderant bacterial bands were identified by gene sequencing. Results:The results revealed that the diversity of fecal dominant microbiota and C. coccoides group in children with CMPA were significantly higher (P < 0.05) than in healthy children. Meanwhile, a lower degree of diversity in the Bifidobacterium group and no difference in the diversity of the Lactobacillus group were found in the feces of children with CMPA. Bacteroides, Clostridium, and Escherichia coli were more abundant in the fecal microbiota of CMPA children than in healthy controls. In addition, C. celerecrescens was found only in the fecal microbiota of CMPA children, whereas B. bifidum was found only in the feces of healthy children. Conclusions: There were some notable changes in the fecal microbiota of 5 -8-year-old children with CMPA.

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Section: Discussionmentioning

confidence: 95%

“…The fecal microbiota characteristics of children with CMPA < 5 years old has been determined; (28) however, studies on the biodiversity of fecal microbiota in children with CMPA > 5 five years old are limited. Importantly, CMPA becomes serious when it affects older children (6, 7).…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Fecal Microbiota in 5 - 8-Year-old Children with and without Cow Milk Protein Allergy

et al. 2016

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“…The resected mucosal biopsies (area = 0.785 cm 2 ) from the distal colon of patients were cut into small pieces, resuspended in 1.4 mL of buffer ASL (QIAamp DNA stool Mini kit, Qiagen, Hilden, Germany), vortexed thoroughly and used for the DNA extraction by using the QIAamp DNA stool Mini kit following the manufacturer's instructions for gram-positive bacteria. DNA extracts were stored at -20℃ until its use.…”

Section: Dna Extractionmentioning

confidence: 99%

“…This intestinal microbiota provides the most important contact with the environment for the host and a barrier against harmful food components and pathogenic bacteria [1,2] . The microbiota has also been shown to have a direct impact on the morphology of the gut [3] and it plays an important role in the intestinal immuno-physiological regulation.…”

Section: Introductionmentioning

confidence: 99%

Qualitative and quantitative analyses of the bifidobacterial microbiota in the colonic mucosa of patients with colorectal cancer, diverticulitis and inflammatory bowel disease

Gueimonde

Ouwehand²,

Huhtinen³

et al. 2007

WJG

106

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AIM:To characterize the bifidobacterial microbiota of the colonic mucosa in patients with colon cancer, inflammatory bowel disease or diverticulitis. METHODS:A sample of the distal colonic mucosa was taken during surgery from a total of 34 patients, twenty-one with diagnosed colorectal cancer, nine with diverticulitis and four with inflammatory bowel disease, requiring surgery for their condition. Bacterial DNA was extracted from the resected mucosal samples and bifidobacterial mucosa-associated microbiota was qualitatively and quantitatively determined by means of qualitative and quantitative PCR. RESULTS:Bifidobacteria were found in 100% of the samples from patients with diverticulitis or IBD and a 76% of those suffering colon cancer. The species B. longum and B. bifidum were the most widely found, followed by B. animalis , B. catenulatum and B. adolescentis . B. breve , B. dentium and B. angulatum were not detected in any sample. A significantly higher occurrence of B. longum was observed in patients with diverticulitis than in those with colon cancer or IBD (100%, 62% and 75%, respectively, P < 0.05). Similar results were obtained for B. animalis (56%, 0% and 25%, P < 0.05), while B. adolescentis was only found in the mucosa from patients with colon cancer (5 out of 21, 24%). At the quantitative level, patients with colon cancer or IBD showed lower counts of total Bifidobacterium (4.94 and 5.91 vs 6.96 log Cells/sample, respectively, P < 0.05) and of the species B. longum (4.05 and 4.79 vs 6.76, P < 0.05) than those with diverticulitis. CONCLUSION:Aberrancies in mucosa associated microbiota are present in different intestinal diseases. This may indicate a role of the microbiota in the pathogenesis of these diseases.

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“…However, the findings of one study are discordant (Sepp et al, 2005) with none of the 5-year-old children with atopic dermatitis and only one child with bronchial asthma colonized with bifidobacteria. Besides, low levels of bifidobacterial colonization have been observed in infants suffering from atopic dermatitis (Kirjavainen et al, 2001;Watanabe et al, 2003;Mah et al, 2006) and in infants suffering from atopic dermatitis and wheezing; note that these results have been contradicted by studies comparing healthy subjects with wheezing infants without other symptoms (Murray et al, 2005) and with patients suffering from both atopic dermatitis and food allergy (Penders et al, 2006a).…”

Section: Microbiota and Allergymentioning

confidence: 54%

Microbiota and Allergy: From Dysbiosis to Probiotics

Waligora‐Dupriet¹,

Butel²

2012

Allergic Diseases - Highlights in the Clinic, Mechanisms and Treatment

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Characterizing the composition of intestinal microflora as a prospective treatment target in infant allergic disease

Cited by 151 publications

References 47 publications

Comparative Analysis of Fecal Microbiota in 5 - 8-Year-old Children with and without Cow Milk Protein Allergy

Comparative Analysis of Fecal Microbiota in 5 - 8-Year-old Children with and without Cow Milk Protein Allergy

Qualitative and quantitative analyses of the bifidobacterial microbiota in the colonic mucosa of patients with colorectal cancer, diverticulitis and inflammatory bowel disease

Microbiota and Allergy: From Dysbiosis to Probiotics

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