Characterizing the binding interactions of PFOA and PFOS with catalase at the molecular level

Xu, Mengchen; Cui, Zhaohao; Zhao, Lining; Hu, Shimeng; Zong, Wansong; Liu, Rutao

doi:10.1016/j.chemosphere.2018.03.200

Cited by 72 publications

(16 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar effects have been observed in somatic cells, where it has been reported that PFOA can increase ROS production and cause deregulation of genes involved in cell division, causing DNA damage and apoptosis 46–50 . The observed increase of ROS in the present study may be based on the damage to the antioxidative system, as it has been previously reported 51 . Our results show that the major source of ROS in PFOA‐exposed CC is the mitochondria.…”

Section: Discussionsupporting

confidence: 91%

“…[46][47][48][49][50] The observed increase of ROS in the present study may be based on the damage to the antioxidative system, as it has been previously reported. 51 Our results show that the major source of ROS in PFOA-exposed CC is the mitochondria. If the antioxidant system is damaged, these ROS could cause lipid peroxidation of mitochondrial membranes causing leakage of ions, which may affect the proton gradient and the oxidative phosphorylation required to produce ATP, thus contributing to further ROS generation.…”

Section: Discussionmentioning

confidence: 59%

See 1 more Smart Citation

Effects of perfluorooctanoic acid in oxidative stress generation, DNA damage in cumulus cells, and its impact on in vitro maturation of porcine oocytes

Teteltitla

Ducolomb

Souza-Arroyo

et al. 2022

Environmental Toxicology

View full text Add to dashboard Cite

Perfluorooctanoic acid is a synthetic compound mostly used in a wide range of consumer products with several adverse effects on somatic cells and gametes. It has been linked to hepatotoxic and carcinogenic effects, alterations in the immune system, endocrine, and reproductive alterations. In vivo studies show an increase in reactive oxygen species and DNA damage. However, the mechanisms by which this compound affects fertility, remain contradictory. Therefore, the aim of the present study was to evaluate the effect of perfluorooctanoic acid on oocyte viability and maturation, as well as the viability, generation of oxidative stress, and genotoxic damage in the cumulus cells exposed during in vitro maturation. This compound had a negative effect on oocyte viability (lethal concentration, LC 50 = 269 μM) and maturation (inhibition maturation concentration IM 50 = 75 μM), while in cumulus cells the LC 50 was 158 μM. The generation of reactive oxygen species evaluated in cumulus cells, protein carbonylation, and DNA damage, was significantly increased at 40 μM perfluorooctanoic acid. This study provides evidence that perfluorooctanoic acid causes reactive oxygen species generation, protein oxidation, and DNA damage in cumulus cells, compromising the maturation and viability of porcine oocyte, which may affect fertility.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 59%

Effects of perfluorooctanoic acid in oxidative stress generation, DNA damage in cumulus cells, and its impact on in vitro maturation of porcine oocytes

Teteltitla

Ducolomb

Souza-Arroyo

et al. 2022

Environmental Toxicology

View full text Add to dashboard Cite

show abstract

“…Like studies in vivo, catalase (CAT) was inhibited by PFOS in vitro at high concentrations, greater than 10 µM. However, PFOA had no significant effect on CAT activity (Xu et al, 2018). The authors also studied binding interactions between both PFCs and CAT and, noting decreased αhelix and increased β-sheets contents in the enzyme structure, inhibition by PFOS could be explained by the bind of the fluorinated compounds close to the enzyme's active site, while the binding site for PFOA was on the surface of the enzyme.…”

Section: Studies Ex Vivo and In Vitro On Fluorinated Organic Compoundsmentioning

confidence: 87%

Enzymatic assays for the assessment of toxic effects of halogenated organic contaminants in water and food. A review

Artabe

Cunha-Silva

Barranco

2020

Food and Chemical Toxicology

View full text Add to dashboard Cite

Halogenated organic compounds are a particular group of contaminants consisting of a large number of substances, and of great concern due to their persistence in the environment, potential for bioaccumulation and toxicity. Some of these compounds have been classified as persistent organic pollutants (POPs) under The Stockholm Convention and many toxicity assessments have been conducted on them previously. In this work we provide an overview of enzymatic assays used in these studies to establish toxic effects and dose-response relationships. Studies in vivo and in vitro have been considered with a particular emphasis on the impact of halogenated compounds on the activity of relevant enzymes to the humans and the environment. Most information available in the literature focuses on chlorinated compounds, but brominated and fluorinated molecules are also the target of increasing numbers of studies. The enzymes identified can be classified as enzymes: i) the activities of which are affected by the presence of halogenated organic compounds, and ii) those involved in their metabolisation/detoxification resulting in increased activities. In both cases the halogen substituent seems to have an important role in the effects observed. Finally, the use of these enzymes in biosensing tools for monitoring of halogenated compounds is described.

show abstract

“…The strong absorption peak at 208 nm is related to the protein backbone, which is caused by the π-π* transition of C=O in the polypeptide chain backbone associated with helix-coil transformation; the peak at 280 nm is related to the absorption of aromatic amino acids. 37,38 As shown in Fig. 3, as the concentration of DHA-S increased, the absorption intensity of HSA at 208 nm was continuously weakened with a slight red shift, but the characteristic absorption peak of aromatic amino acids near 280 nm did not change significantly.…”

Section: Resultsmentioning

confidence: 90%

Probing the potential toxicity by characterizing the binding mechanism of sodium dehydroacetate to human serum albumin

Cui

Xiao

et al. 2021

J Sci Food Agric

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Sodium dehydroacetate (DHA-S) is a common food additive, which can combine with serum proteins in the plasma, but the interaction mechanism between DHA-S and human serum albumin (HSA) is unclear. In this study, multiple spectroscopy techniques, isothermal titration calorimetry (ITC), molecular docking and esterase activity test were employed to investigate the interaction mechanism of DHA-S and HSA. RESULTS: A DHA-S-HSA complex was formed and the structure of HSA were altered by DHA-S. Since DHA-S changed the tight structure of the hydrophobic subdomain IIA where tryptophan (Trp) was placed, the hydrophobicity of the microenvironment of HSA was enhanced. With the addition of DHA-S, the skeleton structure of HSA became loose and the solvent shell on the HSA surface was destroyed. DHA-S altered the secondary structure of HSA, resulting in the decreased ⊍-helix and increased ⊎-sheet contents. The interaction was exothermic and spontaneous driven by van der Waals and hydrogen bonding. DHA-S inhibited the esterase activity of HSA. Molecular docking demonstrated that the binding site of DHA-S on HSA located at the cavity of subdomains IIA and IIIA, but the amino acids related to esterase activity of HSA were not in the binding pocket, indicating that the mechanism by which DHA-S inhibited HSA esterase activity was the change in protein structure.CONCLUSION: This study illustrated that DHA-S interacted with HSA and the structure and function of HSA were affected by DHA-S. This research could help to understand the toxicity of DHA-S and provide basic data for safe use of food additives.

show abstract

Characterizing the binding interactions of PFOA and PFOS with catalase at the molecular level

Cited by 72 publications

References 41 publications

Effects of perfluorooctanoic acid in oxidative stress generation, DNA damage in cumulus cells, and its impact on in vitro maturation of porcine oocytes

Effects of perfluorooctanoic acid in oxidative stress generation, DNA damage in cumulus cells, and its impact on in vitro maturation of porcine oocytes

Enzymatic assays for the assessment of toxic effects of halogenated organic contaminants in water and food. A review

Probing the potential toxicity by characterizing the binding mechanism of sodium dehydroacetate to human serum albumin

Contact Info

Product

Resources

About