Characterizing the Adherence Profiles of Virulent Vibrio parahaemolyticus Isolates

Aagesen, Alisha M.; Phuvasate, Sureerat; Su, Yi‐Cheng; Häse, Claudia C.

doi:10.1007/s00248-017-1025-8

Cited by 8 publications

(6 citation statements)

References 51 publications

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“…For other pathogenic vibrios like V. cholera or V. parahaemolyticus, biofilm production has been linked to virulence or host colonization (Silva and Benitez, 2016;Aagesen et al, 2018). To measure extracellular polysaccharide (EPS) production, indicative of biofilm formation, a modified crystal violet protocol was used based on previous studies (O'Toole, 2011; Guillemette et al, 2020).…”

Section: Bacterial Physiological Testsmentioning

confidence: 99%

Disease Diagnostics and Potential Coinfections by Vibrio coralliilyticus During an Ongoing Coral Disease Outbreak in Florida

et al. 2020

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A deadly coral disease outbreak has been devastating the Florida Reef Tract since 2014. This disease, stony coral tissue loss disease (SCTLD), affects at least 22 coral species causing the progressive destruction of tissue. The etiological agents responsible for SCTLD are unidentified, but pathogenic bacteria are suspected. Virulence screens of 400 isolates identified four potentially pathogenic strains of Vibrio spp. subsequently identified as V. coralliilyticus. Strains of this species are known coral pathogens; however, cultures were unable to consistently elicit tissue loss, suggesting an opportunistic role. Using an improved immunoassay, the VcpA RapidTest, a toxic zinc-metalloprotease produced by V. coralliilyticus was detected on 22.3% of diseased Montastraea cavernosa (n = 67) and 23.5% of diseased Orbicella faveolata (n = 24). VcpA + corals had significantly higher mortality rates and faster disease progression. For VcpA − fragments, 21.6% and 33.3% of M. cavernosa and O. faveolata, respectively, died within 21 d of observation, while 100% of similarly sized VcpA + fragments of both species died during the same period. Further physiological and genomic analysis found no apparent differences between the Atlantic V. coralliilyticus strains cultured here and pathogens from the Indo-Pacific but highlighted the diversity among strains and their immense genetic potential. In all, V. coralliilyticus may be causing coinfections that exacerbate existing SCTLD lesions, which could contribute to the intraspecific differences observed between colonies. This study describes potential coinfections contributing to SCTLD virulence as well as diagnostic tools capable of tracking the pathogen involved, which are important contributions to the management and understanding of SCTLD.

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Section: Bacterial Physiological Testsmentioning

confidence: 99%

Disease Diagnostics and Potential Coinfections by Vibrio coralliilyticus During an Ongoing Coral Disease Outbreak in Florida

et al. 2020

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“…This suggests active accumulation of E. coli O157:H7, which may be due to differential binding of E. coli O157:H7 in the digestive tissue of the mussels. This differential accumulation is also mirrored in Vibrio cholera and V. parahaemolyticus accumulation in mussels and oysters (Collin et al, 2012;Aagesen et al, 2018). This also has parallels with humans where E. coli O157:H7 is known to cause disease in humans via preferential binding to the epithelial cells in the gut due to its high abundance of adhesins and fimbriae (Torres et al, 2005;Vidal et al, 2007).…”

Section: Accumulation Of E Coli In Musselsmentioning

confidence: 88%

E. coli Is a Poor End-Product Criterion for Assessing the General Microbial Risk Posed From Consuming Norovirus Contaminated Shellfish

et al. 2021

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The fecal indicator organism (FIO) Escherichia coli is frequently used as a general indicator of sewage contamination and for evaluating the success of shellfish cleaning (depuration) processes. To evaluate the robustness of this approach, the accumulation, retention, and depuration of non-pathogenic E. coli, pathogenic E. coli O157:H7 and norovirus GII (NoV GII) RNA were evaluated using a combination of culture-based (E. coli) and molecular methods (E. coli, NoV GII) after exposure of mussels (Mytilus edulis) to water contaminated with human feces. We simulated water contamination after a point-source release from a combined sewer overflow (CSO) where untreated wastewater is released directly into the coastal zone. All three microbiological indicators accumulated rapidly in the mussels, reaching close to maximum concentration within 3 h of exposure, demonstrating that short CSO discharges pose an immediate threat to shellfish harvesting areas. Depuration (72 h) in clean water proved partially successful at removing both pathogenic and non-pathogenic E. coli from shellfish tissue, but failed to eradicate NoV GII RNA. We conclude that current EU standards for evaluating microbiological risk in shellfish are inadequate for protecting consumers against exposure to human norovirus GII found in polluted marine waters.

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“…Seafood-borne illnesses and infections because of eating raw or undercooked oysters are caused by V. parahaemolyticus which is a human pathogen [16]. V. parahaemolyticus has led to severe losses in aquaculture in past years.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Vibrio parahaemolyticus Reveals Different Virulence Gene Expression in Response to Benzyl Isothiocyanate

Song

Hou

et al. 2019

Molecules

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Vibrio parahaemolyticus isolated from seafood is a pathogenic microorganism that leads to several acute diseases that are harmful to our health and is frequently transmitted by food. Therefore, there is an urgent need for the control and suppression of this pathogen. In this paper, transcriptional analysis was used to determine the effect of treatment with benzyl isothiocyanate (BITC) extracted from cruciferous vegetables on V. parahaemolyticus and to elucidate the molecular mechanisms underlying the response to BITC. Treatment with BITC resulted in 332 differentially expressed genes, among which 137 genes were downregulated, while 195 genes were upregulated. Moreover, six differentially expressed genes (DEGs) in RNA sequencing studies were further verified by quantitative real-time polymerase chain reaction (qRT-PCR). Genes found to regulate virulence encoded an l-threonine 3-dehydrogenase, a GGDEF family protein, the outer membrane protein OmpV, a flagellum-specific adenosine triphosphate synthase, TolQ protein and VirK protein. Hence, the results allow us to speculate that BITC may be an effective control strategy for inhibiting microorganisms growing in foods.

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Characterizing the Adherence Profiles of Virulent Vibrio parahaemolyticus Isolates

Cited by 8 publications

References 51 publications

Disease Diagnostics and Potential Coinfections by Vibrio coralliilyticus During an Ongoing Coral Disease Outbreak in Florida

Disease Diagnostics and Potential Coinfections by Vibrio coralliilyticus During an Ongoing Coral Disease Outbreak in Florida

E. coli Is a Poor End-Product Criterion for Assessing the General Microbial Risk Posed From Consuming Norovirus Contaminated Shellfish

Transcriptomic Analysis of Vibrio parahaemolyticus Reveals Different Virulence Gene Expression in Response to Benzyl Isothiocyanate

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