Characterizing long lived intralipid-infused tissue phantoms scattering using imaging sensors

Chapman, Glenn H.; Paulsen, S.E.; Zhang, Yutian

doi:10.1117/12.2548689

Cited by 4 publications

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“…In contrast, intralipid solutions may change their optical properties due to factors like temperature changes or particle aggregation, leading to variability in imaging outcomes. 38,39 Moreover, tumor phantoms can replicate the structural characteristics of tumors, including their dimensions, and morphology. By regulating the concentration of fluorophores within these phantoms, concentrations closer to those obtained in vivo mouse models can be achieved.…”

Section: Resultsmentioning

confidence: 99%

“…To investigate this behavior of BSA@SP1 across various tissue types, we utilized 3D tumor-mimicking phantom models using a methodology previously developed by us 32,33 comprising intralipids to simulate fat content (scattering component), hemoglobin to mimic blood vessels (absorption component), and tumor cells labeled with nanoparticles or solely nanoparticles at in vivo concentration levels (fluorescence component). 40,41 It offers distinct advantages over commonly adopted methods, such as utilizing intralipid solutions for simulation 42,43 because tumor-mimicking phantoms can more accurately replicate the optical properties of real tumors, including their scattering and absorption coefficients. As image-guided surgery relies on detecting NIR optical signals, typically from dye uptake, thus, employing phantoms that closely simulate these optical characteristics can yield more precise and realistic outcomes compared to simulations with intralipid solutions, especially when compared to actual in vivo imaging scenarios.…”

Section: Resultsmentioning

confidence: 99%

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Near-Infrared Afterglow Luminescence Amplification via Albumin Complexation of Semiconducting Polymer Nanoparticles for Surgical Navigation in Ex Vivo Porcine Models

Bendele,

Kitamura,

Vasquez

et al. 2024

Preprint

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Afterglow imaging, leveraging persistent luminescence following light cessation, has emerged as a promising modality for surgical interventions. However, the scarcity of efficient near-infrared (NIR) responsive afterglow materials, along with their inherently low brightness and lack of cyclic modulation in afterglow emission, has impeded their widespread adoption. Addressing these challenges requires a strategic repurposing of afterglow materials that improve on such limitations. Here, we have developed an afterglow probe, composed of bovine serum albumin (BSA) coated with an afterglow material, a semiconducting polymer dye (PFODBT/SP1), called BSA@SP1 demonstrating a substantial amplification of the afterglow luminescence (~3-fold) compared to polymer-lipid coated PFODBT (DSPE-PEG@SP1). This enhancement is believed to be attributed to the electron-rich matrix provided by BSA that immobilizes SP1 and enhances the generation of 1O2 radical generation, which is known to enhance the afterglow luminescence brightness. Through molecule docking, physicochemical characterization, and optical assessments, we highlight BSA@SP1 superior afterglow properties, cyclic afterglow behavior, long-term colloidal stability, and biocompatibility. Furthermore, we demonstrate superior tissue permeation profiling of afterglow signals of BSA@SP1 compared to fluorescence signals using ex vivo tumor cell-mimicking phantoms and various porcine tissue types (skin, muscle, and fat). Expanding on this, to showcase BSA@SP1 potential in image-guided surgeries, we implanted tumor-cell phantoms within porcine lungs and conducted direct comparisons between fluorescence and afterglow-guided interventions to illustrate the superiority of the latter. Overall, our study introduces a promising strategy for enhancing current afterglow materials through protein complexation, resulting in both ultrahigh signal-to-background ratios and cyclic afterglow signals.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Near-Infrared Afterglow Luminescence Amplification via Albumin Complexation of Semiconducting Polymer Nanoparticles for Surgical Navigation in Ex Vivo Porcine Models

Bendele,

Kitamura,

Vasquez

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…We previously [14] developed a way of using a high end camera system to take a large number of measurements in a single test using a camera system. In this the laser is mounted on an optical breadboard in front of optical hardware holding the sample aligned to a Digital Single Lens Reflex (DSLR) with a 36 x 24 mm (5676x3674 pixels) image sensor (full frame sensor: a Canon 5D Mark II): see Figure 9 and Figure 10.…”

Section: Measuring Test Phantoms Anistropy G With Digital Cameramentioning

confidence: 99%

Fast scattering coefficient measurement of intralipid-infused tissue phantoms using imaging sensors

Chapman,

Aguilera,

Schilling

2024

Optical Interactions With Tissue and Cells XXXV

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The time instability of regular biological tissue makes repeatable optical tomography imaging experiments through tissue difficult, hence the need for long life tissue phantoms with adjustable scattering parameters. Our test phantoms employ a wide density range of intralipid-infused agar layers 1-6 mm thick building phantoms with small to large scattering parameters of values µs =< 20cm -1 , g = 0.95. The intralipid-infused agar is encapsulated within clear polymer stabilizing these for >10 years creating samples with ranges of thicknesses, scattering characteristics and shapes. To tune phantoms we have created an improved rapid measurement method for scattering coefficients µs and anisotropy factor g. Using a DSLR camera with full frame 36x24mm sensor we 3D printed an optical jig which mounts phantoms like a lens to the camera. Aligning laser beams to the phantom a single picture captures ~6 million pixel values over +/-12 o , creating 20,000 measurements each at 2300 angular bins of 0.005 o . Matlab programs calculate the scattering center and concentric circles of pixels at each angular position. Nonlinear curve fitting the two term Henyey-Greenstein model extracts the pairs of HG parameters: weighing factors, µs and g parameters. Fits are highly statistical significance with exceedingly small deviation from the HG model. Each of 3 test phantom were measured a wide wavelengths range: 405, 532, 632, 670 and NIR 808 nm. The heat mirrors in regular DSLR/Mirror cameras still allow NIR measurements (to ~1000 nm) due to the very low noise of these photography systems.

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Near‐Infrared Afterglow Luminescence Amplification via Albumin Complexation of Semiconducting Polymer Nanoparticles for Surgical Navigation in Ex Vivo Porcine Models

Bendele,

Kitamura,

Vasquez

et al. 2024

Adv Funct Materials

View full text Add to dashboard Cite

Afterglow imaging, leveraging persistent luminescence following light cessation, has emerged as a promising modality for surgical interventions. However, the scarcity of efficient near‐infrared (NIR) responsive afterglow materials, along with their inherently low brightness and lack of cyclic modulation in afterglow emission, has impeded their widespread adoption. Addressing these challenges requires a strategic repurposing of afterglow materials that improve on such limitations. Here, an afterglow probe, composed of bovine serum albumin (BSA) coated with an afterglow material, a semiconducting polymer dye (SP1), called BSA@SP1 demonstrating a substantial amplification of the afterglow luminescence (≈3‐fold) compared to polymer‐lipid coated PFODBT (DSPE‐PEG@SP1) under same experimental conditions is developed. This enhancement is believed to be attributed to the electron‐rich matrix provided by BSA that immobilizes SP1 and enhances the generation of 1O2 radicals, which improves the afterglow luminescence brightness. Through molecular docking, physicochemical characterization, and optical assessments, BSA@SP1's superior afterglow properties, cyclic afterglow behavior, long‐term colloidal stability, and biocompatibility are highlighted. Furthermore, superior tissue permeation profiling of afterglow signals of BSA@SP1's compared to fluorescence signals using ex vivo tumor‐mimicking phantoms and various porcine tissue types (skin, muscle, and fat) is demonstrated. Expanding on this, to showcase BSA@SP1's potential in image‐guided surgeries, tumor‐mimicking phantoms within porcine lungs and conducted direct comparisons between fluorescence and afterglow‐guided interventions to illustrate the latter's superiority is implanted. Overall, the study introduces a promising strategy for enhancing current afterglow materials through protein complexation, resulting in both ultrahigh signal‐to‐background ratios and cyclic afterglow signals.

show abstract

Characterizing long lived intralipid-infused tissue phantoms scattering using imaging sensors

Cited by 4 publications

References 0 publications

Near-Infrared Afterglow Luminescence Amplification via Albumin Complexation of Semiconducting Polymer Nanoparticles for Surgical Navigation in Ex Vivo Porcine Models

Near-Infrared Afterglow Luminescence Amplification via Albumin Complexation of Semiconducting Polymer Nanoparticles for Surgical Navigation in Ex Vivo Porcine Models

Fast scattering coefficient measurement of intralipid-infused tissue phantoms using imaging sensors

Near‐Infrared Afterglow Luminescence Amplification via Albumin Complexation of Semiconducting Polymer Nanoparticles for Surgical Navigation in Ex Vivo Porcine Models

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