Characterizing blast-tolerant transgenic rice constitutively expressing the chitinase of the beta-glucanase gene

Nakazono, K.; Saruta, Masayasu; Kamoshita, Mamoru; Nakajima, Emi; Ugaki, Masashi; Hibi, Tadaharu; Khush, G. S.; Brar, D. S.; Hardy, B.

doi:10.1142/9789812814319_0219

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“…As a typical PR gene, the glycoside hydrolase gene PR‐2 is involved in degradation of the fungal cell wall and has been used in genetic engineering of transgenic rice 23 . Overexpression of the β‐1,3‐glucanase gene OsGns1 in rice was found to enhance resistance to rice blast 31 . Moreover, overexpression of the Chi11 gene and the tobacco‐derived β‐1,3‐glucanase gene in rice enhanced the resistance of transgenic plants to sheath blight 33 .…”

Section: Discussionmentioning

confidence: 99%

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Overexpression of a beta‐1,6‐glucanase gene GluM in transgenic rice confers high resistance to rice blast, sheath blight and false smut

Shen

Wang

et al. 2023

Pest Management Science

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BACKGROUND: Frequent fungal diseases tend to lead to severe losses in rice production. As a main component of the fungal cell wall, glucan plays an important role in the growth and development of fungi. Glucanase can inhibit the growth of fungi by breaking glycosidic bonds, and may be a promising target for developing rice varieties with broad-spectrum disease resistance.RESULTS: We transferred a codon-optimized ⊎-1,6-glucanase gene (GluM) from myxobacteria into the japonica rice variety Zhonghua11 (ZH11), and obtained a large number of individual transgenic plants with GluM overexpression. Based on molecular analysis, three single-copy homozygous lines with GluM overexpression were selected for assessment of fungal disease resistance at the T 3 generation. Compared with that of the recipient cultivar ZH11, the area of rice blast lesion in transgenic rice was reduced by 82.71%; that of sheath blight lesion was decreased by 35.76%-43.67%; the sheath blight resistance in the field was enhanced by an average of 0.75 grade over 3 years; and the incidence of diseased panicles due to rice false smut was decreased by 65.79%. More importantly, there was no obvious loss of yield (without a significant effect on agronomic traits). Furthermore, plants overexpressing a ⊎-1,6-glucanase gene showed higher disease resistance than rice plants overexpressing a ⊎-1,3-glucanase gene derived from tobacco.CONCLUSION: The ⊎-1,6-glucanase gene GluM can confer broad-spectrum disease resistance to rice, providing an environmentally friendly alternative way to effectively manage fungal pathogens in rice production.

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Section: Discussionmentioning

confidence: 99%

“…There are multiple PR‐2 genes in rice. Overexpression of OsGns1 in rice can improve the resistance of plants to rice blast 31 . OsGns6 can be induced by rice blast fungus, and in vitro experiments have demonstrated that the Gns6 protein can inhibit germ tube development and appressorium formation in rice blast fungus 32 .…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a beta‐1,6‐glucanase gene GluM in transgenic rice confers high resistance to rice blast, sheath blight and false smut

Shen

Wang

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

show abstract

Characterizing blast-tolerant transgenic rice constitutively expressing the chitinase of the beta-glucanase gene

Cited by 1 publication

References 0 publications

Overexpression of a beta‐1,6‐glucanase gene GluM in transgenic rice confers high resistance to rice blast, sheath blight and false smut

Overexpression of a beta‐1,6‐glucanase gene GluM in transgenic rice confers high resistance to rice blast, sheath blight and false smut

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