Characterizations of CD4-1, CD4-2 and CD8β T cell subpopulations in peripheral blood leucocytes, spleen and head kidney of Japanese flounder (Paralichthys olivaceus)

Xing, Jing; Ma, Jian; Tang, Xiaoqian; Sheng, Xiuzhen; Zhan, Wenbin

doi:10.1016/j.molimm.2017.02.015

Cited by 54 publications

(33 citation statements)

References 35 publications

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“…Very few CD3ɛ -/CD4-1 + and CD3ɛ -/CD4-2 + cells were observed, which was indicative of CD4 expressed on T lymphocytes in flounder. However, the proportions of CD3 + /CD4-1 + and CD3 + /CD4-2 + T cells were relatively lower than the results we obtained previously 18 . We consider the reason to be related to the stronger specificity of monoclonal antibodies.…”

Section: Expression Of Transcription Factors and Cytokines In Cd4contrasting

confidence: 91%

Kinetics of T lymphocyte subsets and B lymphocytes in response to immunostimulants in flounder (Paralichthys olivaceus): implications for CD4+ T lymphocyte differentiation

Xing

Tian

Tang

et al. 2020

Sci Rep

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CD4 + T lymphocytes play crucial roles in the adaptive immune system. CD4, as the most effective marker to delineate the T-helper subsets, was identified in many fish species. Two CD4 homologs, CD4-1 and CD4-2, have been reported in flounder (Paralichthys olivaceus). In this study, monoclonal antibodies (mAbs) against CD4-1 and CD4-2 of flounder were produced, CD4 + T lymphocytes were isolated and identified, and the variations in CD4 + and CD8 + t lymphocytes and igM + B lymphocytes after Poly I:C, PMA or β-glucan stimulation were investigated. Then, the expression of transcription factors and cytokines in sorted CD4 + T lymphocytes was analyzed. The results showed that the mAbs were specific to flounder CD4-1 + and CD4-2 + T cells. CD4-1 + and CD4-2 + cells responded to all three stimulants, while CD8 + T lymphocytes only give a strong response to Poly I:C, and the percentages of igM + B lymphocytes showed a tendency to increase. After stimulation, the expression of transcription factors and cytokines of Th1, Th2 and Th17 cells varied in CD4 + T cells. These results will provide crucial foundations for the differentiation and function of teleost CD4 + t lymphocytes. CD4 + T cells, also known as T helper (Th) cells, play essential roles in the function of the immune system 1,2. They display extensive functional and phenotypic diversity in response to pathogens and promote cancer surveillance and tolerance to "self " antigens and environmental allergens 2. These functions are performed through specific antigen (Ag) recognition by CD4 + T cells and subsequent secretion of effector and regulatory cytokines 3. Upon Ag encounter, the T cell co-receptor CD4 present on T helper cells binds to the peptide/MHC (pMHC) class II molecules on antigen-presenting cells and transduces activation signals 4,5. Activated CD4 + Th cells can further differentiate into a variety of effector Th cell subsets, such as Th1, Th2, Th17 cells and regulatory T (Treg) cells 6 , and Th1 and Th2 cells were discovered to be the dominant populations of Th cells 7. The differentiation of Th cells towards a Th1 profile occurs in the presence of IL-12 and IFN-γ and is controlled by the master transcription factor T-bet 6. These Th1 cells secrete effector cytokines such as IFN-γ, TNF-a and IL-2 that stimulate macrophages and cytotoxic T lymphocytes (CTLs), which play important roles in cellular immunity against intracellular pathogens 6. Th2 cells, which are promoted by IL-4 and induction of GATA-3, produce cytokines IL-4, IL-5 and IL-13 that stimulate B cells to secrete different antibody isotypes and thus control extracellular infections 6,8,9. Th17 cells produce cytokines, including IL-17, IL-22, and IL-26, and they express the master transcription factor RORγt. They have a role in host defense against extracellular bacteria, fungi, inflammation and autoimmune diseases 10. Treg cells, which are regulated through Foxp3, have an essential role in regulating Th1-, Th2-, and Th17-type responses and are responsible for peripheral tolerance 11. Addition...

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Section: Expression Of Transcription Factors and Cytokines In Cd4contrasting

confidence: 91%

Kinetics of T lymphocyte subsets and B lymphocytes in response to immunostimulants in flounder (Paralichthys olivaceus): implications for CD4+ T lymphocyte differentiation

Xing

Tian

Tang

et al. 2020

Sci Rep

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“…Rabbit anti-flounder CD4-1/CD4-2/IL-2/IL-2Rβ and mouse anti-flounder IL-2 (fIL-2) polyclonal antibodies were produced previously in our laboratory (15)(16)(17). In this study, rabbit anti-flounder CD4-1/CD4-2 polyclonal antibodies (diluted 1:1000) were used for flow cytometry (FCM) and indirect immunofluorescence assay.…”

Section: Antibodiesmentioning

confidence: 99%

Interleukin-2 (IL-2) Interacts With IL-2 Receptor Beta (IL-2Rβ): Its Potential to Enhance the Proliferation of CD4+ T Lymphocytes in Flounder (Paralichthys olivaceus)

et al. 2020

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“…In flounder, T-cell surface molecular marker genes, CD4-1, CD4-2, CD8α, and CD8β, have been cloned and their corresponding specific antibodies produced (9,10). Numerous researchers have studied the cellular immune response resulting from DNA vaccination via analysis of the transcription levels of T-cell marker genes in spleen and head kidney (11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Xing

Tang

et al. 2019

Front. Immunol.

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Vibrio anguillarum is a pathogenic bacterium that infects flounder resulting in significant losses in the aquaculture industry. The VAA protein previously identified in flounder is associated with a role in immune protection within these fish. In the present study, a recombinant DNA plasmid encoding the VAA gene of V. anguillarum was constructed and its potential as a DNA vaccine, to prevent the infection of V. anguillarum in flounder fish, investigated. We verified the expression of the VAA protein both in vitro in cell lines and in vivo in flounder fish. The protective effects of pcDNA3.1-VAA (pVAA) were analyzed by determination of the percentage of sIgM+, CD4-1+, CD4-2+, CD8β+ lymphocytes, and the production of VAA-specific antibodies in flounder following their immunization with the DNA vaccine. Histopathological changes in immune related tissues, bacterial load, and relative percentage survival rates of flounder post-challenge with V. anguillarum, were all investigated to assess the efficacy of the pVAA DNA vaccine candidate. Fish intramuscularly immunized with pVAA showed a significant increase in CD4-1+, CD4-2+, and CD8β+ T lymphocytes at days 9, 11, and 14 post-vaccination, reaching peak T-cell levels at days 11 or 14 post-immunization. The percentage of sIgM+ lymphocytes reached peak levels at weeks 4–5 post-immunization. Specific anti-V. anguillarum or anti-rVAA antibodies were induced in inoculated fish at days 28–35 post-immunization. The liver of vaccinated flounder exhibited only slight histopathological changes compared with a significant pathology observed in control immunized fish. Additionally, a lower bacterial burden in the liver, spleen, and kidney were observed in pVAA protected fish in response to bacterial challenge, compared with pcDNA3.1 vector control injected fish. Moreover, the pVAA vaccine confers a relative percentage survival of 50.00% following V. anguillarum infection. In summary, this is the first study indicating an initial induction of the T lymphocyte response, followed by B lymphocyte induction of specific antibodies as a result of DNA immunization of flounder. This signifies the important potential of pVAA as a DNA vaccine candidate for the control of V. anguillarum infection.

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Characterizations of CD4-1, CD4-2 and CD8β T cell subpopulations in peripheral blood leucocytes, spleen and head kidney of Japanese flounder (Paralichthys olivaceus)

Cited by 54 publications

References 35 publications

Kinetics of T lymphocyte subsets and B lymphocytes in response to immunostimulants in flounder (Paralichthys olivaceus): implications for CD4+ T lymphocyte differentiation

Kinetics of T lymphocyte subsets and B lymphocytes in response to immunostimulants in flounder (Paralichthys olivaceus): implications for CD4+ T lymphocyte differentiation

Interleukin-2 (IL-2) Interacts With IL-2 Receptor Beta (IL-2Rβ): Its Potential to Enhance the Proliferation of CD4+ T Lymphocytes in Flounder (Paralichthys olivaceus)

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

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