Characterization, Stability and Biological Activity In Vitro of Cathelicidin-BF-30 Loaded 4-Arm Star-Shaped PEG-PLGA Microspheres

Bao, Yueli; Wang, Shanrong; Liu, Hongli; Wang, Yunjiao; Chen, Haiyun; Yuan, Mingwei

doi:10.3390/molecules23020497

Cited by 17 publications

(13 citation statements)

References 45 publications

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“…Formulation of this peptide in poly(D,L-lactide-co-glycolide) (PLGA) microspheres allowed for the slow release of cathelicidin-BF in vitro for more than 15 days. Importantly, the microspheres prevented peptide degradation while still maintaining antimicrobial efficacy against E. coli , Shigella dysenteriae , and Salmonella typhi ( Bao et al, 2018 ). In addition, using a murine model of P. aeruginosa pneumonia, intravenous pre-treatment of formulated cathelicidin-BF activated the immune response to improve antibacterial functions while enhancing macrophage clearance and dampening inflammation via obstruction of the NF-κB signaling cascade ( Liu et al, 2018 ).…”

Section: Novel Formulations For Peptide Deliverymentioning

confidence: 99%

Cathelicidin Host Defense Peptides and Inflammatory Signaling: Striking a Balance

et al. 2020

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Host-defense peptides (HDPs) are vital components of innate immunity in all vertebrates. While their antibacterial activity toward bacterial cells was the original focus for research, their ability to modulate immune and inflammatory processes has emerged as one of their major functions in the host and as a promising approach from which to develop novel therapeutics targeting inflammation and innate immunity. In this review, with particular emphasis on the cathelicidin family of peptides, the roles of natural HDPs are examined in managing immune activation, cellular recruitment, cytokine responses, and inflammation in response to infection, as well as their contribution(s) to various inflammatory disorders and autoimmune diseases. Furthermore, we discuss current efforts to develop synthetic HDPs as therapeutics aimed at restoring balance to immune responses that are dysregulated and contribute to disease pathologies.

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Section: Novel Formulations For Peptide Deliverymentioning

confidence: 99%

Cathelicidin Host Defense Peptides and Inflammatory Signaling: Striking a Balance

et al. 2020

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“…The individual scans were corrected by subtracting with the blank. All CD measurements are reported as ellipticities [15,26].…”

Section: Conformational Stability Of Peptidementioning

confidence: 99%

Nano-encapsulated HHC10 host defense peptide (HDP) reduces the growth of Escherichia coli via multimodal mechanisms

Sharma

Vaghasiya

Ray

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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The eradication of several pathogenic drug resistant "Superbug" such as Escherichia coli became difficult especially in chronic infections using existing antibiotics due to the emergence of antibiotic resistance. Owing to their unique antibacterial properties, host defense peptides (HDP) have gained significant attention to combat colonization of bacteria. This study aims designing delivery systems for HHC10 peptide to target bacteria inside the cells might be a promising approach by protecting from degradation, controlling the release, enhancing the susceptibility of target microbes and improving bioavailability. Nano-formulated HHC10 was evaluated for its efficacy (CFU assay) and possible mechanism of action (membrane interaction and apoptosis) against E. coli. Dose-dependent inhibition of E. coli growth is observed for nano-encapsulated and bare HHC10 and encapsulated form remain non-toxic to macrophage mouse cells (RAW264.6) up to 20 μM. Mechanistic analyses using transmission electron microscopy and flow cytometry techniques revealed that bactericidal activity of HHC10-NP progresses via a multimodal mechanism of bacterial cell death by cell-membrane lysis on direct interaction with bacteria while through induction of the apoptotic death pathway inside the host cells. These results offer an insight on future strategies for the development and application of antimicrobial peptides as antibacterial alternatives. [Formula: see text] Controlled delivery of HHC10 peptide from PLGA-NP kills bacteria by two different mechanism: (i) direct killing: HHC10 disintegrate the cell membrane of bacteria by electrostatic interactions and (ii) indirect killing: induction of apoptosis in bacteria infect cells.

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“…Figure 6 shows the results of the assessment of the haemolytic activity of PNS, mPEG-PMA and PNS-loaded microspheres in the concentration range of 0.4–40 μg/mL. PNS, mPEG-PMA and PNS-loaded microspheres all exhibited low haemolytic activity within the set concentration range and the haemolysis rate increased with increasing concentrations but was < 5%, which meets the regulation of the International Standardization Organization (ISO), indicating that the drug-loaded microspheres are safe for contact with blood at a concentration ≤ 40 μg/mL and the drug-loaded microspheres have good biocompatibility [33,34].…”

Section: Resultsmentioning

confidence: 97%

Preparation, In Vivo and In Vitro Release of Polyethylene Glycol Monomethyl Ether-Polymandelic Acid Microspheres Loaded Panax Notoginseng Saponins

Zheng

et al. 2019

Molecules

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In order to enrich the types of Panax notoginseng saponins (PNS) sustained-release preparations and provide a new research idea for the research and development of traditional Chinese medicine sustained-release formulations, a series of Panax notoginseng saponins microspheres was prepared by a double emulsion method using a series of degradable amphiphilic macromolecule materials polyethylene glycol monomethyl ether-polymandelic acid (mPEG-PMA) as carrier. The structure and molecular weight of the series of mPEG-PMA were determined by nuclear magnetic resonance spectroscopy (1 HNMR) and gel chromatography (GPC). The results of the appearance, particle size, drug loading and encapsulation efficiency of the drug-loaded microspheres show that the mPEG10000-PMA (1:9) material is more suitable as a carrier for loading the total saponins of Panax notoginseng. The particle size was 2.51 ± 0.21 μm, the drug loading and encapsulation efficiency were 8.54 ± 0.16% and 47.25 ± 1.64%, respectively. The drug-loaded microspheres were used for in vitro release and degradation experiments to investigate the degradation and sustained release behaviour of the drug-loaded microspheres. The biocompatibility of the microspheres was studied by haemolytic, anticoagulant and cytotoxicity experiments. The pharmacological activity of the microspheres was studied by anti-inflammatory and anti-tumour experiments. The results showed that the drug-loaded microspheres could be released stably for about 12 days and degraded within 60 days. At the same time, the microspheres had good biocompatibility, anti-inflammatory and anti-tumour activities.

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Characterization, Stability and Biological Activity In Vitro of Cathelicidin-BF-30 Loaded 4-Arm Star-Shaped PEG-PLGA Microspheres

Cited by 17 publications

References 45 publications

Cathelicidin Host Defense Peptides and Inflammatory Signaling: Striking a Balance

Cathelicidin Host Defense Peptides and Inflammatory Signaling: Striking a Balance

Nano-encapsulated HHC10 host defense peptide (HDP) reduces the growth of Escherichia coli via multimodal mechanisms

Preparation, In Vivo and In Vitro Release of Polyethylene Glycol Monomethyl Ether-Polymandelic Acid Microspheres Loaded Panax Notoginseng Saponins

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