Characterization of β-hydroxybutyrate transport in rat erythrocytes and thymocytes

Monocarboxylate transport in erythrocytes

“…While the specificity B. Deuticke: Monocarboxylate Transport of this inhibition is less pronounced than first claimed, the presence of the carrier has been firmly established [3,21,22,25,28,32,81,82]. The independent nature of this carrier is demonstrated by its inhibitor sensitivity pattern, substrate specificity, pH dependence and the type of flux coupling.…”

“…Studies on these species have already shown the Cl--system to lack stereoselectivity in monocarboxylate transport -in contrast to the specific monocarboxylate carrier [28] A provisional pattern of monocarboxylates transported by the chloride system is given in Table 3. To what extent unsubstituted monocarboxylates move via the CI-system is unsettled.…”

“…At the levels completely blocking the Cl--system, stilbenedisulfonates produce a saturating but incomplete inhibition of monocarboxylate movements, varying from anion to anion [3,25,28]. SH-group modifying mercurials (PCMBS, PCMB, Hg §247 and dithiol compounds (DTNB, DTDP and several analogues), on the other hand, block that component of monocarboxylate transfer that is insensitive to stilbenedisulfonates, while not affecting -except for DTDP -the C1--system [25,28].…”

“…It has, however, also emerged that aliphatic and aromatic monocarboxylates permeate to a certain extent by the classical anion exchange system [4,18,25,76]. Most recently, convincing evidence has become available that in erythrocytes as in other types of cells aliphatic monocarboxylate transport involves a specialized "carrier" system [3,28,32,46,47]. In the following, the available information on the three parallel mechanisms of monocarboxylate transfer will be summarized, major emphasis being put on the specialized system.…”

LCFA Uptake and FAT/CD36: molecular cloning, tissue expression and mRNA expression responses to dietary oil sources in grass carp (Ctenopharyngodon idellus)

“…The grass carp is a warm water fish species, whereas the main essential FAs for this species are 18:2n-6 and 18:3n-3 (Takeuchi et al 1991) while the Atlantic salmon is mainly a marine fish species with important requirements in n-3 HUFA, especially EPA and DHA (NRC 1993). In general terms, the membrane proteins, FABPpm, FAT/CD36 and fatty acid transport protein (FATP), are responsible for the cellular uptake of LCFAs (Frohnert and Bernlohr 2000) and phloretin was reported to be a potent non-specific inhibitor of membrane transport proteins (Lefevre 1975;Andersen et al 1978). This meant that the buffer containing this inhibitor would suppress the efflux or influx of FAs across the cell membrane by blocking the activity of these proteins (Abumrad et al 1981).…”