Characterization of Β-Glucan Extracted From Saccharomyces Cereviseae and Candida Albicans

Abstract

β-glucan is a polysaccharide with β-glycosidic linkages and it's a major structural component of various yeast cells. The goal of this research was to focus on the characterization of β-glucan extracted from both types of yeasts (Saccharomyces cereviseae and Candida albicans).The extraction of β-glucan depend on acid-base hydrolysis method and the yield of β-glucan extracted from Saccharomyces cereviseae was 63.35gm (28.15%) while the yield of βglucan extracted from Candida albicans was 9.35gm (4.15%) from 225gm of both yeasts also the results explained that High performance liquid chromatography (HPLC) analysis of extracted β-glucan had similarity to the standard of β-glucan in major peak 3.22 of liquid samples extracted from two types of yeasts. On the other hand, Scanning electron microscope (SEM) revealed that standard of β-glucan was similar to both β-glucan extracted from Saccharomyces cereviseae and Candida albicans in size of particles in range (1.11-2.60µm) and no presence of cell wall trace but different in morphology.

Characterization of β-glucan obtained from Candida albicans of caprine mastitis

“…The current study’s findings are consistent with those of Jameel and Yassein (2021) who extracted β-glucan utilizing a technique based on Alkaline-acidic extraction stages with (4.15%); also, the morphological characteristics of the C. albicans derived β-glucan powder recorded as yellow crystal particles. In addition, Al-Jumaiee et al (2019) reported a yield of 5.95% from 4 g of the same yeast.…”

“…The results of a study by Jameel and Yassein (2021) performed by SEM on the particles of conventional β-glucan demonstrated the existence of homogeneous, irregular aggregating generating irregular mass with cylindrical to round particles The findings of very few studies on SEM for β-glucan obtained from C. albicans showed that the particles of β-glucan obtained from C. albicans having an aggregation of booklet-like platelet having particle sizes ranging from 1.2 to 1.84 µm.…”

“…The same peak (at 3.78) was recorded by Ibrahim (2014) when analyzing the β-glucans obtained from Saccharomyces cerevisiae by HPLC. According to Jameel and Yassein (2021) , the extracted β-glucan’s HPLC analysis result showed its structural resemblance to the β-glucan standard. The β-glucans obtained from S. cerevisiae and C. albicans displayed a large peak at 3.22, which represents the pureness of the β-glucans isolated from both fungi.…”