Characterization of zymosan-induced arthritis in the rat: Effects on joint inflammation and cartilage metabolism

Gegout, P.; Gillet, Pierre; Chevrier, D.; Guingamp, Corinne; Terlain, Bernard; Netter, Patrick

doi:10.1016/0024-3205(94)00771-3

Cited by 53 publications

(47 citation statements)

References 12 publications

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“…In previous study, Gegout et al (20) observed that intraarticular injection of zymosan increased the body temperature. The present study showed that this effect was, in fact, characterized as an integrated febrile response since the increase in rectal temperature was preceded by a decrease in the tail skin temperature.…”

Section: Discussionmentioning

confidence: 90%

“…Frasnelli et al (19) demonstrated that Toll-like receptor (TLR) 2 is the major pathway of proinflammatory signaling in zymosan-induced arthritis in mice. Furthermore, this stimulus has been used in the investigation of other aspects of arthritis-associated inflammatory response, including loss of mobility, articular hyperalgesia, edema, and fever (20,21,46,47,52).…”

Section: Discussionmentioning

confidence: 99%

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Characterization and pharmacological evaluation of febrile response on zymosan-induced arthritis in rats

Kanashiro¹,

Pessini²,

Machado³

et al. 2009

American Journal of Physiology-Regulatory, Integrative and Comp

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Kanashiro A, Pessini AC, Machado RR, Malvar DC, Aguiar FA, Soares DM, Vale ML, Souza GEP. Characterization and pharmacological evaluation of febrile response on zymosan-induced arthritis in rats. Am J Physiol Regul Integr Comp Physiol 296: R1631-R1640, 2009. First published February 25, 2009 doi:10.1152/ajpregu.90527.2008.-The present study investigated the febrile response in zymosan-induced arthritis, as well as the increase in PGE2 concentration in the cerebrospinal fluid (CSF), along with the effects of antipyretic drugs on these responses in rats. Zymosan intra-articularly injected at the dose of 0.5 mg did not affect the body core temperature (Tc) compared with saline (control), whereas at doses of 1 and 2 mg, zymosan promoted a flattened increase in Tc and declined thereafter. The dose of 4 mg of zymosan was selected for further experiments because it elicited a marked and long-lasting Tc elevation starting at 3 1/2 h, peaking at 5 1/2 h, and remaining until 10 h. This temperature increase was preceded by a decrease in the tail skin temperature, as well as hyperalgesia and edema in the knee joint. No febrile response was observed in the following days. In addition, zymosan-induced fever was not modified by the sciatic nerve excision. Zymosan increased PGE 2 concentration in the CSF but not in the plasma. Oral pretreatment with ibuprofen (5-20 mg/kg), celecoxib (1-10 mg/kg), dipyrone (60 -240 mg/kg), and paracetamol (100 -200 mg/kg) or subcutaneous injection of dexamethasone (0.25-1.0 mg/kg) dose-dependently reduced or prevented the fever during the zymosan-induced arthritis. Celecoxib (5 mg/kg), paracetamol (150 mg/kg), and dipyrone (120 mg/kg) decreased CSF PGE 2 concentration and fever during zymosan-induced arthritis, suggesting the involvement of PGE2 in this response.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Characterization and pharmacological evaluation of febrile response on zymosan-induced arthritis in rats

Kanashiro¹,

Pessini²,

Machado³

et al. 2009

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Experimental arthritis was induced on day 0 in ZYM-injected, NPXtreated, and DEX-treated rats by IA injection of ZYM (2 mg in 50 l of 0.9% saline; Sigma, St. Louis, MO) in both knees. This chemical agent induces a subacute synovitis resulting in a significant loss of glycosaminoglycans (14)(15)(16). At the same time, control rats received an IA injection of 50 l saline in both knees.…”

Section: Methodsmentioning

confidence: 99%

Effects of antiinflammatory drugs on arthritic cartilage: A high‐frequency quantitative ultrasound study in rats

Jaffre¹,

Watrin²,

Lœuille³

et al. 2003

Arthritis & Rheumatism

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Objective. To evaluate the ability of 55-MHz quantitative ultrasound (US) to detect the in vivo effects of experimental arthritis, as well as those of two antiinflammatory drugs, naproxen (NPX) and dexamethasone (DEX), on cartilage and subchondral bone.Methods. Arthritis was induced in both knees of 108 rats by intraarticular injection of zymosan (ZYM). Two groups of arthritic rats (n ‫؍‬ 36 per group) were treated daily with either NPX (10 mg/kg/day) or DEX (0.1 mg/kg/day). Using a 3-dimensional US microscope, patellae were explored in vitro on days 5, 14, and 21 after injections. US assessment included the analysis of quantitative indices of local modifications involving cartilage and bone: integrated reflection coefficient (IRC) from the cartilage surface and apparent integrated backscatter from the cartilage internal structure (cartilage matrix) (AIB cartilage ) and the cartilage-bone interface (AIB bone ).Results. ZYM induced articular surface fibrillation that resulted in a decrease in IRC at all times (P < 0.02) and in an increase in AIB bone on days 5 and 14 (P < 0.005). Fibrillation was not changed by NPX administration, while it disappeared following DEX treatment. Cartilage-bone interface alterations were prevented by DEX and partially compensated for by NPX. Cartilage matrix echogenicity decreased with time in all groups due to maturation (P < 0.05), except in DEX-treated rats.Conclusion. Quantitative 55 MHz US allowed detection of early cartilage and bone lesions due to experimental arthritis, and also allowed detection of the effects of antiinflammatory drugs. NPX seemed to have an effect on subchondral bone lesions, but not on cartilage. DEX appeared to repair articular surface and bone, but prevented animal growth and cartilage maturation.In rheumatoid arthritis (RA), synovial inflammation and pannus releasing matrix metalloproteinases, oxygen-derived free radicals, cytokines, and prostaglandins degrade articular cartilage. These mediators provoke proteoglycan (PG) synthesis inhibition, cartilage destruction, bone erosions, and ancillary progressive articular loss of function (1,2). They are also responsible for the swelling and pain in arthritic joints. Nonsteroidal antiinflammatory drugs (NSAIDs) and oral steroids reduce the RA symptoms of joint pain and swelling. However, evidence in clinics and from in vitro studies that NSAIDs and steroids can prevent the progression of chronic, destructive arthritis is both sparse and controversial (3-7). Furthermore, most in vitro studies rely on destructive techniques (e.g., histology and biochemistry), and the criteria for determining cartilage degradation remain qualitative.There is increasing interest in the use of ultrasonography in rheumatology, since ultrasound (US) is noninvasive, safe, more accurate than clinical evaluation of synovitis and cartilage thickness in small joints, and can be used repeatedly in RA patients (8). Recent research suggests that high-frequency US may also serve ex vivo as a useful means for the investigation of cartilage ma...

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“…Acute Zy arthritis, up to the first three days following induction, is characterized by increase in vascular permeability and cell migration. Later, a progressive synovitis takes place, with mononuclear cell infiltration and fibroblast reaction resembling chronic rheumatoid synovitis [4]. Previous studies suggest that factors governing gait disturbance may be diverse from the ones involved in other inflammatory phenomena, such as alteration in vascular permeability and cell influx.…”

Section: Introductionmentioning

confidence: 99%

Periarthritis promotes gait disturbance in zymosan-induced arthritis in rats

et al. 1999

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Characterization of zymosan-induced arthritis in the rat: Effects on joint inflammation and cartilage metabolism

Cited by 53 publications

References 12 publications

Characterization and pharmacological evaluation of febrile response on zymosan-induced arthritis in rats

Characterization and pharmacological evaluation of febrile response on zymosan-induced arthritis in rats

Effects of antiinflammatory drugs on arthritic cartilage: A high‐frequency quantitative ultrasound study in rats

Periarthritis promotes gait disturbance in zymosan-induced arthritis in rats

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