Characterization of Yeast Strains of the Genera Candida, Hansenula, Kluyveromyces, Pichia, Rhodotorula, and Saccharomyces by Mixed-Dye Fluorometry

Abstract

An analytical method in which we used the selective adsorption of several fluorophores by yeast cells is described. The suitability of using binary mixtures of 1-pyrene butyric acid, 3,6-dimethylamino acridine, 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid, and rhodamine B isothiocyanate for the characterization and identification of microorganisms was tested with 98 yeast strains belonging to the genera Candida, Hansenulu, Kluyveromyces, Pichia, Rhodotorula, and Saccharomyces. The application of multivariate statistical methods and pattern recognition methods to the allocation of the yeast strains into genus-species-strain structures and to a comparison of fluorescence data sets for differentiation and identification purposes showed _-

Capillary Isoelectric Focusing and Fluorometric Detection of Proteins and Microorganisms Dynamically Modified by Poly(ethylene glycol) Pyrenebutanoate

“…The detection of microorganisms is often based on the light scattering in the UV or visible region, but it is not sensitive enough to detect low concentrations of MOs. , Fluorescence detection has often been applied for the trace analysis of biological analytes. The selective adsorption of fluorophores on the cells was used in fluorescence spectroscopy, video fluorometry, fluorescence, , or confocal laser microscopy, and in flow cytometry . In CIEF, the fluorogenic reagents are used for covalent or noncovalent protein labeling using pre-, on-, or postcolumn derivatization or “indirect” fluorometry .…”