Characterization of yeast groupings in the phyllosphere of Sphagnum mosses

Yeasts from peat in a tropical peat swamp forest in Thailand and their ability to produce ethanol, indole-3-acetic acid and extracellular enzymes

“…In the present study, R. mucilaginosa was the most frequently isolated yeast (21 strains, 32.3 % of all strains) in peat. Among the ten species detected in this study, only R. mucilaginosa and P. laurentii are known to have been isolated from peatland in Russia and Canada (Kachalkin and Yurkov 2012;Kachalkin et al 2008;Thormann et al 2007). R. mucilaginosa is one of the most, or perhaps the most, ubiquitous basidiomycetous yeast, found in a wide range of habitats (Sampaio 2011).…”

“…Cryptococcus (ten species), Candida (eight species), Pichia (five species) and Rhodotorula (four species) were reported as the most prevalent genera, accounting for 58 % of known species. Kachalkin et al (2008) investigated the epiphytic yeast communities formed on Sphagnum mosses on peat in Moscow, Russia, and reported that among various known yeast species R. mucilaginosa was included. Kachalkin and Yurkov (2012) studied the yeast communities in Sphagnum mosses phyllosphere in boreal forest and swamp biotopes in the Tver region, Russia and found a significantly higher relative abundance of ascomycetous yeasts on swamp moss.…”

“…To date, there have been a limited number of articles reporting the microbial composition of tropical peat swamp forests (Boonyuen et al 2012;Bunterngsook et al 2010;Hadi et al 2012;Jackson et al 2009;Pinnoi et al 2006;Posa et al 2011) and temperate and boreal peatlands (Dedysh et al 2006; GrumSection Editor: Eckhard Thines Electronic supplementary material The online version of this article (doi:10.1007/s11557-016-1205-9) contains supplementary material, which is available to authorized users. Grzhimaylo et al 2016;Kachalkin and Yurkov 2012;Kachalkin et al 2008;Thormann et al 2007). Among these, only a few articles have reported yeasts in peatlands of Canada and Russia, and their ecological roles are largely unknown (GrumGrzhimaylo et al 2016;Kachalkin and Yurkov 2012;Kachalkin et al 2008;Thormann et al 2007).…”

“…Grzhimaylo et al 2016;Kachalkin and Yurkov 2012;Kachalkin et al 2008;Thormann et al 2007). Among these, only a few articles have reported yeasts in peatlands of Canada and Russia, and their ecological roles are largely unknown (GrumGrzhimaylo et al 2016;Kachalkin and Yurkov 2012;Kachalkin et al 2008;Thormann et al 2007).…”

Ogataea cecidiorum sp. nov., a methanol-assimilating yeast isolated from galls on willow leaves

“…Mini-and microsatellite-specific PCR fingerprints and Restriction Fragments Length Polymorphism analysis were performed as described before (Yurkov and Chernov 2005;Kachalkin et al 2008). For sequencing of the D1/D2 region of the 26S ribosomal gene and the internal transcribed spacers (ITS), DNA fragments were amplified with the ITS1f (5 0 -CTT GGT CAT TTA GAG GAA GTA) and NL4 (5 0 -GGT CCG TGT TTC AAG ACG G) primer pair using a program consisting of an initial denaturation step of 2 min at 96°C, followed by 35 cycles of 20 s at 96°C, 50 s 52°C and 1.5 min at 72°C and a final extension step of 7 min at 72°C.…”