Characterization of X-chromosome specific satellite DNA of Muntiacus muntjak vaginalis

Bogenberger, Jakob; Schnell, Hannelore; Fittler, Friedrich

doi:10.1007/bf00333506

Cited by 19 publications

(7 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using plasmid DNA of this BAC clone as template, we obtained products from two pairs of primer combination. One pair is two telomere-specific primers (C 3 TA 2 ) 3 (TeloC) and (T 2 AG 3 ) 3 (TeloG), the other is (C 3 TA 2 ) 3 (TeloC) against the Indian muntjac DNA satellite IA (SatIA, Bogenberger et al 1982). The former G-and Crich telomere primers resulted in a smear, which is consistent with the previous conclusion that the template contains telomeric repeats (Hartmann and Scherthan 2004).…”

Section: Rapid Evolution Of Genes In the Indian Muntjacsupporting

confidence: 88%

Comparative genomic analysis links karyotypic evolution with genomic evolution in the Indian Muntjac (Muntiacus muntjak vaginalis)

et al. 2006

View full text Add to dashboard Cite

The karyotype of Indian muntjacs (Muntiacus muntjak vaginalis) has been greatly shaped by chromosomal fusion, which leads to its lowest diploid number among the extant known mammals. We present, here, comparative results based on draft sequences of 37 bacterial artificial clones (BAC) clones selected by chromosome painting for this special muntjac species. Sequence comparison on these BAC clones uncovered sequence syntenic relationships between the muntjac genome and those of other mammals. We found that the muntjac genome has peculiar features with respect to intron size and evolutionary rates of genes. Inspection of more than 80 pairs of orthologous introns from 15 genes reveals a significant reduction in intron size in the Indian muntjac compared to that of human, mouse, and dog. Evolutionary analysis using 19 genes indicates that the muntjac genes have evolved rapidly compared to other mammals. In addition, we identified and characterized sequence composition of the first BAC clone containing a chromosomal fusion site. Our results shed new light on the genome architecture of the Indian muntjac and suggest that chromosomal rearrangements have been accompanied by other salient genomic changes.

show abstract

Section: Rapid Evolution Of Genes In the Indian Muntjacsupporting

confidence: 88%

Comparative genomic analysis links karyotypic evolution with genomic evolution in the Indian Muntjac (Muntiacus muntjak vaginalis)

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Since the probe used here was a consensus sequence based upon a population of variable 31-mers, we may assume that both levels of periodicity (i.e., 31-bp and 1.4 kb) have been preserved since the Bovini last shared a common ancestor, about 2-4 MY ago (Groves 1981). The 1.4-kb periodicity that was seen in the Bovini, yet found to be absent in other taxa, is consistent with the results of Bogenberger et al (1985Bogenberger et al ( , 1987, who isolated a satellite called IA from the Indian muntjak, a cervid not examined in the present study. They found that the IA satellite had a 31-bp core motif whose consensus is identical at 29 of the 31 positions to that of the bovine 1.715 satellite consensus and that it was present in cervids and bovids but absent in tragulids.…”

Section: Conservation Of Repetitive Sequences and Systematic Implicatsupporting

confidence: 80%

Evolutionary histories of highly repeated DNA families among the artiodactyla (mammalia)

1996

View full text Add to dashboard Cite

Six highly repeated DNA families were analyzed using Southern blotting and fluorescence in situ hybridization in a comparative study of 46 species of artiodactyls belonging to seven of the eight extant taxonomic families. Two of the repeats, the dispersed bovine-Pst family and the localized 1.715 component, were found to have the broadest taxonomic distributions, being present in all pecoran ruminants (Giraffidae, Cervidae, Antilocapridae, and Bovidae), indicating that these repeats may be 25-40 million years old. Different 1.715 restriction patterns were observed in different taxonomic families, indicating that independent concerted evolution events have homogenized different motifs in different lineages. The other four satellite arrays were restricted to the Bovini and sometimes to the related Boselaphini and Tragelaphini. Results reveal that among the two compound satellites studied, the two components of the 1.711a originated simultaneously, whereas the two components of the 1.711b originated at two different historical times, perhaps as many as 15 million years apart. Systematic conclusions support the monophyly of the infraorder Pecora, the monophyly of the subfamily Bovinae (containing the Boselaphini, Bovini, and Tragelaphini), an inability to resolve any interrelationships among the other tribes of bovids, paraphyly of the genus Bos with respect to Bison, and a lack of molecular variation among two morphologically and ecologically distinct subspecies of African buffaloes (Syncerus caffer cafer and S. c. nanus). Cytogenetically, a reduction in diploid chromosome numbers through centric fusion in derived karyotypes is accompanied by a loss of centromeric satellite DNA. The nilgai karyotype contains an apparent dicentric chromosome as evidenced by the sites of 1.715 hybridization. Telomeric sequences have been translocated to the centromeres without concomitant chromosomal rearrangement in Thompson's gazelle.

show abstract

“…If so this should have led to direct fusion of telomere and satellite sequences. To test for the presence and to isolate such fusion junctions we designed PCR primers for Indian muntjac DNA satellite IA (Bogenberger et al 1982) (primer SatIA) and Chinese muntjac DNA satellite I (C5; Lin et al 1991) (primer Munt2) and combined each of these satellite DNA primers with one telomere-specific primer (T 2 AG 3 ) 3 (TeloG) or (C 3 TA 2 ) 3 (TeloC). These primer combinations were assumed to amplify an ancestral fusion point when satellite and telomere DNA sequences were fused and sufficiently close.…”

Section: Resultsmentioning

confidence: 99%

Characterization of ancestral chromosome fusion points in the Indian muntjac deer

Hartmann

Scherthan

2004

Chromosoma

View full text Add to dashboard Cite

Tandem fusion, a rare evolutionary chromosome rearrangement, has occurred extensively in muntjac karyotypic evolution, leading to an extreme fusion karyotype of 6/7 (female/male) chromosomes in the Indian muntjac. These fusion chromosomes contain numerous ancestral chromosomal break and fusion points. Here, we designed a composite polymerase chain reaction (PCR) strategy which recovered DNA fragments that contained telomere and muntjac satellite DNA sequence repeats. Nested PCR confirmed the specificity of the products. Two-color fluorescence in situ hybridization (FISH) with the repetitive sequences obtained and T2AG3 telomere probes showed co-localization of satellite and telomere sequences in Indian muntjac chromosomes. Adjacent telomere and muntjac satellite sequences were also seen by fiber FISH. These data lend support to the involvement of telomere and GC-rich satellite DNA sequences during muntjac chromosome fusions.

show abstract

Characterization of X-chromosome specific satellite DNA of Muntiacus muntjak vaginalis

Cited by 19 publications

References 32 publications

Comparative genomic analysis links karyotypic evolution with genomic evolution in the Indian Muntjac (Muntiacus muntjak vaginalis)

Comparative genomic analysis links karyotypic evolution with genomic evolution in the Indian Muntjac (Muntiacus muntjak vaginalis)

Evolutionary histories of highly repeated DNA families among the artiodactyla (mammalia)

Characterization of ancestral chromosome fusion points in the Indian muntjac deer

Contact Info

Product

Resources

About