Characterization of WSSV resistance in selected families of Litopenaeus vannamei

Huang, Yuyang; Yin, Zhimin; Ai, Hua-Shu; Huang, Xian-De; Li, Sedong; Weng, Shaoping; He, Jianguo

doi:10.1016/j.aquaculture.2010.11.032

Cited by 44 publications

(24 citation statements)

References 16 publications

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“…In recent decades, numerous studies have focused on the pathogenesis of WSSV and the host immune response, and have carried out selective breeding programs (Huang et al, 2011;Cuellar-Anjel et al, 2012). Although there have been advances in studies about viral structure and related immune responses of shrimp (Tang et al, 2007;, the detailed mechanisms of interaction between WSSV and the host remain unclear.…”

Section: Discussionmentioning

confidence: 98%

A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance

et al. 2014

Chin. J. Ocean. Limnol.

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Anti-lipopolysaccharide factors (ALFs) are basic components of the crustacean immune system that defend against a range of pathogens. The cDNA sequence of a new ALF, designated nLvALF2 , with an open reading frame encoding 132 amino acids was cloned. Its deduced amino acid sequence contained the conserved functional domain of ALFs, the LPS binding domain (LBD). Its genomic sequence consisted of three exons and four introns. nLvALF2 was mainly expressed in the Oka organ and gills of shrimps. The transcriptional level of nLvALF2 increased signifi cantly after white spot syndrome virus (WSSV) infection, suggesting its important roles in protecting shrimps from WSSV. Single nucleotide polymorphisms (SNPs) were found in the genomic sequence of nLvALF2 , of which 38 were analyzed for associations with the susceptibility/resistance of shrimps to WSSV. The loci g.2422 A>G, g.2466 T>C, and g.2529 G>A were signifi cantly associated with the resistance to WSSV ( P <0.05). These SNP loci could be developed as markers for selection of WSSV-resistant varieties of Litopenaeus vannamei .

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Section: Discussionmentioning

confidence: 98%

A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance

et al. 2014

Chin. J. Ocean. Limnol.

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“…It is distributed in the eastern Pacific Ocean from northern Peru, through the equator, to the Gulf of Mexico (Rendon, Macias, Calderon, Nunez & Solis ). The output is the highest for penaeid shrimp, making up approximately 52% of the total shrimp output in the world (Briggs, Funge‐Smith, Subasinghe & Phillips ; Huang, Yin, Ai, Huang, Li, Weng & He ). Litopenaeus vannamei was introduced into China in 1988 (Ruan, Luo, Luan, Kong, Xu, Chen & Chen ) and now, after 20 years of practical experience, it has become a dominant variety in China.…”

Section: Introductionmentioning

confidence: 99%

Heritability and genotype by environment interactions for growth and survival inLitopenaeus vannameiat low and high densities

et al. 2016

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Genetic parameters and genotype by rearing density interactions for growth traits and survival were investigated in Litopenaeus vannamei. A total of 3626 shrimps from 40 families in two densities (low density, 80 shrimps m À2 ; high density, 160 shrimps m À2 ) were measured for four growth traits, body length, body weight, cephalothorax length and abdominal segment length. Variance components and genetic parameters for growth and survival were estimated using a two-trait animal model or a threshold (probit) sire and dam model. The common environment effect was excluded because it could not be partitioned effectively, likely because of high heritability. The heritability for growth traits varied from 0.21 to 0.44, indicating a moderate level. For survival, the backtransformed heritability (h 2 p ) was 0.36 AE 0.06 and 0.22 AE 0.04 for the low and high densities respectively. There were no significant differences in heritability for growth traits and survival between the two densities (P > 0.05). High genetic correlations (0.94-0.98) between the two densities for growth traits indicated a low genotype by density interaction and ranking effect across the environments, which suggested that these traits were controlled mostly by the same genes over the two densities. Therefore, selection in a low-density environment should produce desired correlated responses for growth at a high density. Genetic correlation (0.77 AE 0.09) between the two densities for survival was moderate and significant, which indicated that the genotype by density interaction effect was moderate and low for survival under the two densities. Our results provide crucial information for use in L. vannamei breeding programmes in China.

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“…However, recent efforts appear more promising. For example, Huang et al (2010) reported producing families of L. vannamei with a mean survival of 22.7% after three generations of selection for WSSV resistance, and Cuéllar-Anjel et al (2011) reported producing families of L. vannamei with survival ranging from 23% to 57% after WSSV exposure.…”

Section: Breeding For Disease Resistancementioning

confidence: 96%