Characterization of Whole Blood Aggregation with a New Type of Aggregometer by a Screen Filtration Pressure Method

Ozeki, Yasushi; Sudo, Toshiki; Toga, Kouhei; Nagamura, Yoshie; Ito, Hideki; Ogawa, Takeo; Kimura, Yukio

doi:10.1016/s0049-3848(00)00377-7

Cited by 38 publications

(51 citation statements)

References 6 publications

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“…Comparison of Platelet Aggregation with a Screen Filtration Pressure Method-Measurements of platelet aggregation with a screen filtration pressure aggregometer (WBA analyzer from SSR Engineering Co., Ltd., Yokohama, Japan) were performed according to a method described previously (13,14). Reaction tubes containing 200-l aliquots of whole blood were placed in an incubation chamber at 37°C for 30 s followed by the addition of 22.2 l of a serial concentration (1, 2, 4, 8 or 16 M) of ADP (Sigma).…”

Section: Methodsmentioning

confidence: 99%

Disruption of Nuclear Vitamin D Receptor Gene Causes Enhanced Thrombogenicity in Mice

Aihara¹,

Azuma²,

Akiyama³

et al. 2004

Journal of Biological Chemistry

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233

161

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Vitamin D metabolites influence the expression of various genes involved in calcium homeostasis, cell differentiation, and regulation of the immune system. Expression of these genes is mediated by the activation of the nuclear vitamin D receptor (VDR). Previous studies have shown that a hormonally active form of vitamin D, 1␣,25-dihydroxyvitamin D3, exerts anticoagulant effects in cultured monocytic cells. To clarify whether activation of VDR plays any antithrombotic actions in vivo, hemostatic/thrombogenic systems were examined in normocalcemic VDR knock-out (KO) mice on a high calcium diet and compared with wild type and hypocalcemic VDRKO mice that were fed a regular diet. Platelet aggregation was enhanced significantly in normocalcemic VDRKO mice compared with wild type and hypocalcemic VDRKO mice. Aortic endothelial nitric-oxide (NO) synthase expression and urinary NOx excretions were reduced in hypocalcemic VDRKO mice, but not in normocalcemic VDRKO mice. Northern blot and RT-PCR analyses revealed that the gene expression of antithrombin in the liver as well as that of thrombomodulin in the aorta, liver and kidney was down-regulated in hypo-and normocalcemic VDRKO mice. Whereas tissue factor mRNA expression in the liver and kidney was up-regulated in VDRKO mice regardless of plasma calcium level. Furthermore, VDRKO mice manifested an exacerbated multi-organ thrombus formation after exogenous lipopolysaccharide injection regardless of the calcemic conditions. These results demonstrate that activation of nuclear VDR elicits antithrombotic effects in vivo, and suggest that the VDR system may play a physiological role in the maintenance of antithrombotic homeostasis.

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Section: Methodsmentioning

confidence: 99%

Disruption of Nuclear Vitamin D Receptor Gene Causes Enhanced Thrombogenicity in Mice

Aihara¹,

Azuma²,

Akiyama³

et al. 2004

Journal of Biological Chemistry

Self Cite

233

161

View full text Add to dashboard Cite

show abstract

“…To overcome this, several methods of measuring platelet aggregability directly in whole blood have been developed. One of them is the screen filtration pressure (SFP) method, [8][9][10] in which whole-blood aggregability is analyzed by measuring the absorbing pressure of agonist-stimulated whole blood through a microsieve. We have shown in previous study of healthy subjects that the antiplatelet effect of aspirin can be clearly evaluated with this method.…”

Section: Editorial P 1077mentioning

confidence: 99%

Characterization of the Antiplatelet Effect of Aspirin at Enrollment and After 2-Year Follow-up in a Real Clinical Setting in Japan

Ikeda

Taniguchi

Watanabe

et al. 2010

Circ J

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Background:Aspirin is an antiplatelet drug widely used for the prevention of cardiovascular diseases. It has been reported that some patients who exhibit a reduced antiplatelet effect of aspirin have higher cardiovascular risk. It is still controversial whether the antiplatelet effect of aspirin diminishes after a few years of treatment. This study aimed to evaluate the antiplatelet effect of aspirin and its 2-year change in Japanese patients. Methods and Results:Collagen-induced platelet-aggregability was measured at enrollment by conventional optical aggregometer in 239 patients undergoing antiplatelet therapy with aspirin alone. Among them, 167 patients were evaluated after 2 years. Whole blood aggregability based on the screen-filtration method was also evaluated. Optical aggregometer studies showed that 27% of patients were low-responders. Multivariate analyses revealed that female sex and non-use of calcium-channel blockers were associated with low responsiveness. The antiplatelet effect of aspirin did not decrease after 2 years. Similar data were obtained with the whole blood aggregometer. Conclusions:In this Japanese patient group, 27% were low-responders to aspirin, and the antiplatelet effect of aspirin did not decrease after a 2-year interval. (Circ J 2010; 74: 1227 - 1235

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“…In our study, we confirmed an additional finding, which is that platelet aggregation function assays are useful for detecting patients at high risk for bleeding events. In particular, whole blood aggregation analysis using a screen filtration pressure method has good reproducibility and easy handling 12,13) and requires only a short time. Hence, we presume it would be suitable as a monitoring instrument in an outpatient clinic.…”

Section: Discussionmentioning

confidence: 99%

“…Study procedures were conducted according to the method described by Ozeki et al 12) and Sudo et al 13) . Briefly, whole blood aggregation was induced by adding 8 M adenosine diphosphate (ADP) to 200 L whole blood.…”

Section: Platelet Function Measurementmentioning

confidence: 99%

Platelet Function Measured Using a Whole Blood Aggregometer Can Predict Bleeding Events

Sairaku¹,

Nakano

Eno³

et al. 2011

JAT

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Aim:We hypothesized that excessive suppression of platelet function due to antiplatelet therapy can increase the incidence of bleeding complications. The aim of the present study was to find whether we could predict bleeding events by measuring platelet function. Methods: We enrolled 743 subjects whose platelet function was measured using a whole blood aggregometer based on a screen filtration pressure method. Of these subjects, 551 (74.2%) were treated with some type of antiplatelet agent. The endpoints were bleeding or ischemic events requiring hospitalization or extension of hospital stay. We prospectively compared the platelet function of subjects with and without bleeding or ischemic events.

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Characterization of Whole Blood Aggregation with a New Type of Aggregometer by a Screen Filtration Pressure Method

Cited by 38 publications

References 6 publications

Disruption of Nuclear Vitamin D Receptor Gene Causes Enhanced Thrombogenicity in Mice

Disruption of Nuclear Vitamin D Receptor Gene Causes Enhanced Thrombogenicity in Mice

Characterization of the Antiplatelet Effect of Aspirin at Enrollment and After 2-Year Follow-up in a Real Clinical Setting in Japan

Platelet Function Measured Using a Whole Blood Aggregometer Can Predict Bleeding Events

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