Characterization of wheat/Aegilops ventricosa introgression and addition lines with respect to the Mv genome

Mena, Montaña; Orellana, Juan; López-Braña, I.; Garcı́a-Olmedo, Francisco; Delibes, A.

doi:10.1007/bf00222079

Cited by 18 publications

(15 citation statements)

References 19 publications

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“…ventricosa AP-1 to the H-93-8 line, a 5M v (5A) and 7M v (7D) substitution line, obtained from the cross [(Triticum turgidum Ae. ventricosa) T. aestivum] Mena et al 1993;Ogbonnaya et al 2001). The Cre2 gene located on an unknown M v chromosome, confers a high level of resistance to populations of H. avenae Ha71 (Spanish), Ha11 (British) and Fr1-Fr4 races (French), but was ineffective against HgI and HgIII (both Swedish) and the Australian Ha13 Ogbonnaya et al 2001).…”

Section: Introductionmentioning

confidence: 98%

“…This species is an allotetraploid (2n = 4x = 28) with the genomic constitution D v D v M v M v , which is partially homologous to that of T. aestivum (AABBDD). Genes from the D v genome are more easily transferred by recombination with the D genome, than those from the homoeologous but less-closely related M v genome (Doussinault et al 1983;Mena et al 1993). So far, three dominant resistance genes, Cre2, Cre5 and Cre6, have been transferred to wheat germplasm from two different accessions of Ae.…”

Section: Introductionmentioning

confidence: 99%

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Biochemical and genetic studies of two Heterodera avenae resistance genes transferred from Aegilops ventricosa to wheat

Montes¹,

López-Braña²,

Balseiro-Romero

et al. 2003

Theor Appl Genet

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Two Heterodera avenae resistance genes, Cre2 from Aegilops ventricosa AP-1 and Cre5 from Ae. ventricosa #10, were shown to confer a high level of resistance to the Spanish pathotype Ha71. No susceptible plants were found in the F(2) progeny from the cross between the two accessions of Ae. ventricosa, suggesting that their respective resistance factors were allelic. However, genes Cre2 and Cre5 apparently were transferred to a different chromosomal location in the wheat line H-93-8 and in the 6M(v)(6D) substitution, respectively, as proved by F(2) segregation of their cross progeny. The induction of several defence responses during early infection by the same H. avenae pathotype in resistant lines carrying Cre2 or Cre5 genes was studied. Isoelectrofocusing (IEF) isozyme analysis revealed that peroxidase, esterase and superoxide dismutase activity increased after nematode infection, in roots of resistant lines in comparison with their susceptible parents. Differential induced isoforms were also identified when IEF patterns of resistant lines were compared. A DNA marker, absent in Cre5-carrying genotypes, was found to be linked, thought not very tightly, to the Cre2 gene in the H-93-8 line. The differences observed between the Cre2 and Cre5 genes with respect to their chromosomal location in wheat introgression lines, de-toxificant enzyme induction and behaviour against different pathotypes, suggest they are different H. avenae resistance sources for wheat breeding.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Biochemical and genetic studies of two Heterodera avenae resistance genes transferred from Aegilops ventricosa to wheat

Montes¹,

López-Braña²,

Balseiro-Romero

et al. 2003

Theor Appl Genet

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“…ventricosa substitution line, H-93-33 (resistant to Hessian fly), derived from the cross [(T. turgidum H-1-1 ¥ Ae. ventricosa AP-1) ¥ T. aestivum H-10-15] was described previously (Mena et al 1989(Mena et al , 1993Delibes et al 1997). Hexaploid wheat (T. aestivum) cultivars from ''The Uniform Hessian fly Nursery'' (UHFN), with resistance to different biotypes of M. destructor, were kindly supplied by Dr. Bockelman and F. Maas from USDA-ARS.…”

Section: Biological Materialsmentioning

confidence: 99%

A new Hessian fly resistance gene (H30) transferred from the wild grass Aegilops triuncialis to hexaploid wheat

et al. 2003

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A new Hessian fly (Mayetiola destructor Say) resistance gene from Aegilops triuncialis and its transfer to hexaploid wheat via interspecific hybridisation is described. The transfer line TR-3531 (42 chromosomes), derived from the cross [(Triticum turgidum x Ae. triuncialis) x Triticum aestivum] and carrying the Heterodera avenae resistance gene Cre7, showed a high level of resistance to the M. destructor biotype prevailing in the SW of Spain. A single dominant gene (H30) seems to determine the Hessian fly resistance in this introgression line, and its linkage with an isozyme marker (Acph-U1) has also been studied. It has been demonstrated that the resistance gene H30 in the TR-3531 line is non-allelic with respect to the genes H3, H6, H9, H11, H12, H13, H18 and H21, present in wheat cultivars from the Uniform Hessian Fly Nursery (UHFN), as well as to H27, carried by the introgression line H-93-33. Advanced lines with the H30 gene were obtained by backcrossing the transfer line and different commercial wheats as recurrent parents. Several of them showed a high yield in tests carried out in the infested field. Electronic Supplementary Material is available if you access this article at http://dx.doi.org/10.1007/s00122-002-1182-z. On that page (frame on the left side), a link takes you directly to the supplementary material.

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“…ventricosa to the cultivated wheat T. aestivum (Doussinault et al, 1983;Mena et al, 1993 this cross generated fertile and stable lines with 42 chromosomes after subsequent repeated selfing.…”

Section: Wheat/aegilops Introgression Linesmentioning

confidence: 99%

Characterization and selection of hexaploid wheats containing resistance to Heterodera avenae or Mayetiola destructor introgressed from Aegilops: a review

Delibes¹,

Braña²,

Vázquez³

2008

Span. j. agric. res.

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Cereal cyst nematode (CCN, Heterodera avenae) and Hessian fly (HF, Mayetiola destructor) are two major pests affecting wheat crops worldwide including important cereal areas of Spain. Aegilops ventricosa and Ae. triuncialis were used as donors in a strategy to introduce resistance genes (RG) for these pests in hexaploid wheat (Triticum aestivum L.). Two 42 chromosomes introgression lines have been derived from Ae. ventricosa: H-93-8 and H-93-33 carrying genes Cre2 and H27 conferring resistance to CCN and HF, respectively. Line TR-3531 with 42 chromosomes has been derived from Ae. triuncialis and carries RGs conferring resistance for CCN (Cre7) and for HF (H30). Alien material has been incorporated in lines H-93 by chromosomal substitution and recombination, while in line TR-3531 homoeologous recombination affecting small DNA fragments has played a major role. It has been demonstrated that Cre2, Cre7, H27 and H30 are major single dominant genes and not allelic of other previously described RGs. Biochemical and molecular-biology studies of the defense mechanism triggered by Cre2 and Cre7 have revealed specific induction of peroxidase and other antioxidant enzymes. In parallel to these basic studies advanced lines carrying resistance genes for CNN and/or HF have been developed. Selection was done using molecular markers for eventually «pyramiding» resistance genes. Several isozyme and RAPD markers have been described and, currently, new markers based on transposable elements and NBS-LRR sequences are being developed. At present, two advanced lines have already been included at the Spanish Catalogue of Commercial Plant Varieties.Additional key words: Aegilops triuncialis, Aegilops ventricosa, cereal cyst nematode, Hessian fly, resistance genes, Triticum aestivum. Resumen Revisión. Caracterización y selección de trigos hexaploides con resistencia a Heterodera avenae o Mayetiola destructor transferida desde AegilopsEl nematodo del quiste de los cereales (CCN, Heterodera avenae) y el mosquito del trigo (HF, Mayetiola destructor) son dos plagas que afectan a los cultivos de trigo incluyendo importantes áreas cerealistas en España. Aegilops ventricosa y Ae. triuncialis se han usado como donantes en una estrategia para introducir genes de resistencia (RG) para estas plagas en trigo hexaploide (Triticum aestivum L.). Por una parte, a partir de Ae. ventricosa se han generado dos líneas de introgresión de 42 cromosomas: H-93-8 y H-93-33, con los genes Cre2 y H27 que confieren resistencia a CCN y HF, respectivamente. Por otra, se ha generado la línea TR-3531 de 42 cromosomas, derivada de Ae. triuncialis, con RGs para CCN (Cre7) y HF (H30). La introgresión en las líneas H-93 se ha producido por sustitución cromosómica y recombinación, mientras que en la línea TR-3531 ha sido fundamentalmente por la recombinación homeóloga de pequeños fragmentos de ADN. Cre2, Cre7, H27 y H30 son genes dominantes simples, no alélicos a otros RGs previamente descritos. Estudios bioquímicos y de biología molecular sobre las defensas induci...

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Characterization of wheat/Aegilops ventricosa introgression and addition lines with respect to the Mv genome

Cited by 18 publications

References 19 publications

Biochemical and genetic studies of two Heterodera avenae resistance genes transferred from Aegilops ventricosa to wheat

Biochemical and genetic studies of two Heterodera avenae resistance genes transferred from Aegilops ventricosa to wheat

A new Hessian fly resistance gene (H30) transferred from the wild grass Aegilops triuncialis to hexaploid wheat

Characterization and selection of hexaploid wheats containing resistance to Heterodera avenae or Mayetiola destructor introgressed from Aegilops: a review

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