In New England, seasonal forest ponds provide primary breeding habitat for several amphibian species, including Rana sylvatica (LeConte) and Ambystoma maculatum (Shaw). Because each species requires a minimum duration of inundation to complete its breeding cycle, one of the most important factors determining habitat suitability is a pond's hydroperiod. The objective of this research was to develop a method for estimating pond hydroperiod from site characteristics such as pond morphology, geology, chemistry, and vegetation structure, and to use the estimates to assess the suitability of individual ponds for breeding amphibians. We monitored the duration of surface inundation in 65 ponds in the Pawcatuck River Canopy cover, open basin depth, and specific conductance of surface water were among the most useful site characteristics for estimating hydroperiod, while surficial geology and the texture of soil parent material made smaller contributions. The CCR using open basin depth alone was 95.4 and 73.8%, respectively. These findings indicate that it is possible to estimate the hydroperiod of seasonal ponds -and to assess their suitability for individual species of breeding amphibians -without prolonged periods of hydrologic monitoring. Such techniques could have considerable value to wetland regulatory agencies and for planning amphibian habitat management and acquisition at the landscape scale.