Characterization of wavy root 1, an agravitropism allele, reveals the functions of OsPIN2 in fine regulation of auxin transport and distribution and in ABA biosynthesis and response in rice (Oryza sativa L.)

Li, Wenqiang; Zhang, Minjuan; Qiao, Lei; Chen, Yunbo; Zhang, David; Jing, Xiu-Qing; Gan, Pengfei; Huang, Yangbin; Gao, Junru; Liu, Wenting; Shi, Chao; Cui, Hongchang; Li, Haifeng; Chen, Kun-Ming

doi:10.1016/j.cj.2021.12.004

Cited by 15 publications

(34 citation statements)

References 54 publications

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“…In line with this, we also noticed that NPA or BFA treatment could partially or almost completely abolish the curly root phenotype ( Figure 11 ), emphasizing the involvement of polar auxin transport in modulating ospin1b root gravitropism. A recent report further confirmed that the wavy root phenotype, caused by the loss of function of OsPIN2 , indeed resulted from the disrupted polar auxin transport in root tips, which then led to the alteration of auxin levels, auxin distribution and decreased amyloplast sedimentation in columella cells [ 45 ].…”

Section: Discussionmentioning

confidence: 94%

“…There are 12 PIN genes in the rice genome: four OsPIN1 ( OsPIN1a - OsPIN1d ), one OsPIN2 , three OsPIN5 ( OsPIN5a - OsPIN5c ), one OsPIN8 , and three monocot-specific PIN genes, OsPIN9 , OsPIN10a , and OsPIN10b [ 17 , 19 ]. To date, several OsPIN genes have been cloned and functionally characterized, such as the OsPIN1 subfamily [ 3 , 7 , 38 ], OsPIN2 [ 39 , 40 , 41 , 42 , 43 , 44 , 45 ], OsPIN5b [ 46 ] and OsPIN10a [ 47 ]. A previous study showed that rice OsPIN1b is involved in the regulation of adventitious root emergence and tiller number [ 3 ], while further investigation reported that ospin1 single mutants had no dramatic phenotypes and only pin1a pin1b or pin1c pin1d double mutants showed obvious phenotypes, indicating the functional redundancy of OsPIN1 genes in modulating rice architecture [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, OsPIN1b is also involved in nitric oxide (NO) or strigolactones (SLs)-induced root elongation under low-nitrogen and -phosphate conditions [ 38 ]. OsPIN2 is involved in root elongation growth, root gravitropic responses and lateral root formation patterns via regulating the basipetal auxin flow from the root tip towards the root-elongation zone [ 42 , 43 , 45 ]. OsPIN5b protein targets the endoplasmic reticulum and is involved in modulating tiller number, root system, panicle length and grain yield in rice [ 46 ].…”

Section: Introductionmentioning

confidence: 99%

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Mutation of OsPIN1b by CRISPR/Cas9 Reveals a Role for Auxin Transport in Modulating Rice Architecture and Root Gravitropism

Ouyang

Yang

Zhang

et al. 2022

IJMS

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The distribution and content of auxin within plant tissues affect a variety of important growth and developmental processes. Polar auxin transport (PAT), mainly mediated by auxin influx and efflux transporters, plays a vital role in determining auxin maxima and gradients in plants. The auxin efflux carrier PIN-FORMED (PIN) family is one of the major protein families involved in PAT. Rice (Oryza sativa L.) genome possesses 12 OsPIN genes. However, the detailed functions of OsPIN genes involved in regulating the rice architecture and gravity response are less well understood. In the present study, OsPIN1b was disrupted by CRISPR/Cas9 technology, and its roles in modulating rice architecture and root gravitropism were investigated. Tissue-specific analysis showed that OsPIN1b was mainly expressed in roots, stems and sheaths at the seedling stage, and the transcript abundance was progressively decreased during the seedling stages. Expression of OsPIN1b could be quickly and greatly induced by NAA, indicating that OsPIN1b played a vital role in PAT. IAA homeostasis was disturbed in ospin1b mutants, as evidenced by the changed sensitivity of shoot and root to NAA and NPA treatment, respectively. Mutation of OsPIN1b resulted in pleiotropic phenotypes, including decreased growth of shoots and primary roots, reduced adventitious root number in rice seedlings, as well as shorter and narrower leaves, increased leaf angle, more tiller number and decreased plant height and panicle length at the late developmental stage. Moreover, ospin1b mutants displayed a curly root phenotype cultured with tap water regardless of lighting conditions, while nutrient solution culture could partially rescue the curly root phenotype in light and almost completely abolish this phenotype in darkness, indicating the involvement of the integration of light and nutrient signals in root gravitropism regulation. Additionally, amyloplast sedimentation was impaired in the peripheral tiers of the ospin1b root cap columella cell, while it was not the main contributor to the abnormal root gravitropism. These data suggest that OsPIN1b not only plays a vital role in regulating rice architecture but also functions in regulating root gravitropism by the integration of light and nutrient signals.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mutation of OsPIN1b by CRISPR/Cas9 Reveals a Role for Auxin Transport in Modulating Rice Architecture and Root Gravitropism

Ouyang

Yang

Zhang

et al. 2022

IJMS

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“…ABI4 may also be key to integrating ABA, auxin, and reactive oxygen species (ROS) signaling via ASCORBATE PEROXIDASE 6 (APX6) in seeds [ 171 , 172 ] and controls auxin levels through upstream involvement of YUC4 [ 173 ], although the precise mechanisms are not yet clear. In rice, the OsPIN2 mutant wavy root 1 ( war1 ) is defective in auxin transport and auxin distribution at root tips, leading to loss of gravitropic perception, but also shows increased sensitivity to ABA in seed germination, increased ABA levels, and changes in ABA-associated gene expression in roots [ 174 ]. A subsequent study later revealed that ABA reduces the amount of PIN2 in the membrane by suppressing the expression of PIN2 rather than accelerating the degradation of PIN2 [ 140 ].…”

Section: Plant Hormone Crosstalkmentioning

confidence: 99%

Do Opposites Attract? Auxin-Abscisic Acid Crosstalk: New Perspectives

Ortiz‐García

Ortega-Villaizán

Onejeme

et al. 2023

IJMS

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Plants are constantly exposed to a variety of different environmental stresses, including drought, salinity, and elevated temperatures. These stress cues are assumed to intensify in the future driven by the global climate change scenario which we are currently experiencing. These stressors have largely detrimental effects on plant growth and development and, therefore, put global food security in jeopardy. For this reason, it is necessary to expand our understanding of the underlying mechanisms by which plants respond to abiotic stresses. Especially boosting our insight into the ways by which plants balance their growth and their defense programs appear to be of paramount importance, as this may lead to novel perspectives that can pave the way to increase agricultural productivity in a sustainable manner. In this review, our aim was to present a detailed overview of different facets of the crosstalk between the antagonistic plant hormones abscisic acid (ABA) and auxin, two phytohormones that are the main drivers of plant stress responses, on the one hand, and plant growth, on the other.

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“…ABI4 may also be key to integrating ABA, auxin, and reactive oxygen species (ROS) signaling via ASCORBATE PEROXIDASE 6 (APX6) in seeds [167,168] and controls auxin levels through upstream involvement of YUC4 [169], although the precise mechanisms are not yet clear. In rice, the OsPIN2 mutant wavy root 1 (war1) is defective in auxin transport and auxin distribution at root tips, leading to loss of gravitropic perception, but also shows increased sensitivity to ABA in seed germination, increased ABA levels, and changes in ABA-associated gene expression in roots [170]. A subsequent study later revealed that ABA reduces the amount of PIN2 in the membrane by suppressing the expression of PIN2 rather than accelerating the degradation of PIN2 [136].…”

Section: Further Examples For Auxin-aba Crosstalkmentioning

confidence: 99%

Do Opposites Attract? New Insight into the Crosstalk between Auxin and ABA.

Ortiz‐García¹,

Ortega-Villaizán²,

Onejeme³

et al. 2023

Preprint

View full text Add to dashboard Cite

Plants are constantly exposed to a variety of different environmental stresses, including drought, salinity, and elevated temperatures. These stress cues are assumed to aggravate in the future driven by the global climate change scenario which we are currently experiencing. These stressors have largely detrimental effects on plant growth and development and, therefore, put global food security in jeopardy. For this reason, it is necessary to expand our understanding of the underlying mechanisms by which plants respond to abiotic stresses. Especially boosting our insight into the ways by which plants balance their growth and their defense programs appears to be of paramount importance, as this may lead to novel perspectives that can pave the way to increase agricultural productivity in a sustainable manner. In this review, our aim was to present a detailed overview of different factettes of the crosstalk between the antagonistic plant hormones abscisic acid (ABA) and auxin, two phytohormones that are the main drivers of plant stress responses, on the one hand, and plant growth, on the other.

show abstract

Characterization of wavy root 1, an agravitropism allele, reveals the functions of OsPIN2 in fine regulation of auxin transport and distribution and in ABA biosynthesis and response in rice (Oryza sativa L.)

Cited by 15 publications

References 54 publications

Mutation of OsPIN1b by CRISPR/Cas9 Reveals a Role for Auxin Transport in Modulating Rice Architecture and Root Gravitropism

Mutation of OsPIN1b by CRISPR/Cas9 Reveals a Role for Auxin Transport in Modulating Rice Architecture and Root Gravitropism

Do Opposites Attract? Auxin-Abscisic Acid Crosstalk: New Perspectives

Do Opposites Attract? New Insight into the Crosstalk between Auxin and ABA.

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