Characterization of water-soluble hemicelluloses from spruce and aspen employing SEC/MALDI mass spectroscopy

Jacobs, Anna; Lundqvist, Jon; Stålbrand, Henrik; Tjerneld, Folke; Dahlman, Olof

doi:10.1016/s0008-6215(02)00054-x

Cited by 72 publications

(52 citation statements)

References 17 publications

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“…(1/4)-b-DGlucomannan does not contain long stretches of mannan or glucan residues. Instead, it has alternated Man and Glc residues with Glc:Man ratios of 1:1 to 1:3 (Timell, 1986;Jacobs et al, 2002). This alternated polymerization of Man and Glc in glucomannan synthesis was also projected by the radioactivity products after enzymatic hydrolysis of the in vitro glucomannan (Fig.…”

Section: Ptcsla1 Likely Encodes a Key Glcmans For The Synthesis Of Womentioning

confidence: 91%

“…Cellulose is a linear polymer composed of (1/4)-linked b-D-Glc residues. Xylan is a polymer with a linear backbone composed entirely of b-D-Xyl residues connected through (1/4)-linkages and is partially acetylated and substituted with 4-Omethyl-GlcUA groups (Perila, 1961;Timell, 1964Timell, , 1969Fengel and Wegener, 1984;Jacobs et al, 2002). Xylan in angiosperm wood is therefore also referred to as O-acetyl-4-O-methylglucuronoxylan.…”

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confidence: 99%

“…Xylan in angiosperm wood is therefore also referred to as O-acetyl-4-O-methylglucuronoxylan. Glucomannan in angiosperm wood is essentially a pure linear polymer containing (1/4)-linked b-D-Glc and b-D-Man residues, with Glc:Man ratios of 1:1 to 1:3 (Timell, 1986;Jacobs et al, 2002).…”

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confidence: 99%

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The Cellulose Synthase Gene Superfamily and Biochemical Functions of Xylem-Specific Cellulose Synthase-Like Genes inPopulus trichocarpa

et al. 2006

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Wood from forest trees modified for more cellulose or hemicelluloses could be a major feedstock for fuel ethanol. Xylan and glucomannan are the two major hemicelluloses in wood of angiosperms. However, little is known about the genes and gene products involved in the synthesis of these wood polysaccharides. Using Populus trichocarpa as a model angiosperm tree, we report here a systematic analysis in various tissues of the absolute transcript copy numbers of cellulose synthase superfamily genes, the cellulose synthase (CesA) and the hemicellulose-related cellulose synthase-like (Csl) genes. Candidate Csl genes were characterized for biochemical functions in Drosophila Schneider 2 (S2) cells. Of the 48 identified members, 37 were found expressed in various tissues. Seven CesA genes are xylem specific, suggesting gene networks for the synthesis of wood cellulose. Four Csl genes are xylem specific, three of which belong to the CslA subfamily. The more xylem-specific CslA subfamily is represented by three types of members: PtCslA1, PtCslA3, and PtCslA5. They share high sequence homology, but their recombinant proteins produced by the S2 cells exhibited distinct substrate specificity. PtCslA5 had no catalytic activity with the substrates for xylan or glucomannan. PtCslA1 and PtCslA3 encoded mannan synthases, but PtCslA1 further encoded a glucomannan synthase for the synthesis of (1→4)-β-d-glucomannan. The expression of PtCslA1 is most highly xylem specific, suggesting a key role for it in the synthesis of wood glucomannan. The results may help guide further studies to learn about the regulation of cellulose and hemicellulose synthesis in wood.

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Section: Ptcsla1 Likely Encodes a Key Glcmans For The Synthesis Of Womentioning

confidence: 91%

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confidence: 99%

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The Cellulose Synthase Gene Superfamily and Biochemical Functions of Xylem-Specific Cellulose Synthase-Like Genes inPopulus trichocarpa

et al. 2006

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“…The degree of acetylation varies based on the type of tissue and plant species; it was reported that the degree of acetylation varied between 0.60 and 0.75 in glucuronoxylan of aspen wood and 0.3 and 0.4 in galactoglucomannan of spruce, aspen and birch wood (Teleman et al 2000, 2003). The acetyl groups were also found to migrate between the neighbouring free hydroxyl groups and the positions of which varies based on the species (Pauly 1999; Jacobs et al 2002; Mastihubová and Biely 2004) (Table 1). …”

Section: Introductionmentioning

confidence: 99%

Understanding the structural and functional properties of carbohydrate esterases with a special focus on hemicellulose deacetylating acetyl xylan esterases

Kameshwar

Qin

2018

Mycology

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Acetyl and methyl esterifications are two major naturally found substitutions in the plant cell-wall polysaccharides. The non-cellulosic plant cell-wall polysaccharides such as pectin and hemicellulose are differentially esterified by the O-acetyl and methyl groups to cease the action of various hydrolytic enzymes secreted by different fungi and bacterial species. Thus, microorganisms have emerged with a special class of enzymes known as carbohydrate esterases (CE). The CE catalyse O-de, N-deacetylation of acetylated saccharide residues (esters or amides, where sugars play the role of alcohol/amine/acid). Carbohydrate active enzyme (CAZy) database has classified CE into 16 classes, of which hemicellulose deacetylating CE were grouped into eight classes (CE-1 to CE-7 and CE-16). Various plant biomass degrading fungi and bacteria secretes acetyl xylan esterases (AcXE); however, these enzymes exhibit varied substrate specificities. AcXE and xylanases-coupled pretreatment methods exhibit significant applications, such as enhancing animal feedstock, baking industry, production of food additives, paper and pulp, xylitol production and biorefinery-based industries, respectively. Thus, understanding the structural and functional properties of acetyl xylan esterase will significantly aid in developing the efficient AcXE with wide range of industrial applications.

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“…Based on MALDI-TOF mass spectrometric data, 25 we calculated the degree of substitution on average and their standard deviations for succinate and acetate as 3.42 ± 0.72 and 0.91 ± 0.31, respectively. For investigation of the effect of substituents attached to α-C18, succinates and acetates of highly substituted α-C18 were removed by alkaline treatment.…”

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confidence: 99%

Chiral Separation of Catechin by Capillary Electrophoresis with α-Cyclosophorooctadecaose Isolated from Rhodobacter sphaeroides as Chiral Selectors

Kwon¹,

Jeong²,

Jung

2011

Bulletin of the Korean Chemical Society

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Characterization of water-soluble hemicelluloses from spruce and aspen employing SEC/MALDI mass spectroscopy

Cited by 72 publications

References 17 publications

The Cellulose Synthase Gene Superfamily and Biochemical Functions of Xylem-Specific Cellulose Synthase-Like Genes inPopulus trichocarpa

The Cellulose Synthase Gene Superfamily and Biochemical Functions of Xylem-Specific Cellulose Synthase-Like Genes inPopulus trichocarpa

Understanding the structural and functional properties of carbohydrate esterases with a special focus on hemicellulose deacetylating acetyl xylan esterases

Chiral Separation of Catechin by Capillary Electrophoresis with α-Cyclosophorooctadecaose Isolated from Rhodobacter sphaeroides as Chiral Selectors

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