Characterization of Vocal Fold Scarring in a Canine Model

Rousseau, Bernard; Hirano, Shigeru; Scheidt, Troy D.; Welham, Nathan V.; Thibeault, Susan L.; Chan, Roger W.; Bless, Diane M.

doi:10.1097/00005537-200304000-00007

Cited by 191 publications

(238 citation statements)

References 15 publications

(34 reference statements)

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“…9 On the other hand, in a chronic scarring model of the vocal fold, the density of HA in the scarred vocal folds seemed to vary among animal studies. In rabbit and canine studies, HA density showed no significant difference in scar compared with normal vocal folds at both two months and six months, 7 whereas HA was found to be significantly reduced in scarred vocal folds with a three-month rat model. 12 These results indicate variations of HA due to species differences and time between insult and evaluation of scar.…”

Section: Human Scarred Vocal Folds 11mentioning

confidence: 81%

Histologic Characterization of Human Scarred Vocal Folds

et al. 2009

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Objectives: Vocal fold scarring remains a significant problem. Although several animal models have been developed to improve our understanding of the histopathology, the histologic features of scarred human vocal folds have rarely been reported. The present case studies aimed to define the histologic changes of scarred human vocal folds caused by cordectomy or cordotomy.Methods: Ten patients with the scarred vocal folds were involved in this study. Nine patients with early glottic cancer underwent endoscopic cordectomy, and one patient underwent superficial cordotomy for idiopathic scar. The post-cordectomy or cordotomy scar was biopsied or resected 3 to 13 months after the original procedure. After confirming absence of any tumor in cancer patients, the remaining specimens were used in the present study. Histologic examination investigated deposition of extracellular matrix (ECM) including collagen, elastin, hyaluronic acid (HA), fibronectin, and decorin in the lamina propria of the scarred vocal folds.Results: There was a wide range variation in the deposition of ECM in scarred vocal folds.Excessive and disorganized collagen deposition was observed in most cases that had undergone deep resection of the lamina propria, whereas deposition of collagen was mild and well organized after superficial resection. Decorin was retained in all cases after superficial cordectomy or cordotomy, but varied after deep resection. Deposition of elastin, HA, and fibronectin varied regardless of depth of injury. Conclusion:Histology of scarred vocal folds may vary with degree of injury and individual healing mechanism.

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Section: Human Scarred Vocal Folds 11mentioning

confidence: 81%

Histologic Characterization of Human Scarred Vocal Folds

et al. 2009

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“…This period of vocal fold scar maturation was based on data from Rousseau et al, who proposed that it takes 6 months for vocal fold scarring to mature in canine and rat models. 3,4 Six months after the procedure, 0.5 mL of hydrogel solution containing 1 lg of HGF was injected into the scarred vocal folds of four dogs (HGF-treated group) using a transoral intracordal injector, and 0.5 mL of hydrogel solution containing 1 lg of PBS was injected into the scarred vocal folds of the four dogs in the sham group. Because HGF was expected to act on the fibroblast in the lamina propria, hydrogel was carefully injected into the subepithelial layer of the vocal fold.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Chronic vocal fold scar restoration with hepatocyte growth factor hydrogel

et al. 2009

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Objectives/Hypothesis: Therapeutic challenges exist in the management of vocal fold scarring. We have previously demonstrated the therapeutic potential of hepatocyte growth factor (HGF) in the management of acute phase vocal fold scarring using a novel hydrogel-based HGF drug delivery system (DDS). However, the effect of HGF on matured vocal fold scarring remains unclear. The current study aims to investigate the effect of HGF-DDS on chronic vocal fold scarring using a canine model.Study Design: Animal model. Methods: Vocal folds from eight beagles were unilaterally scarred by stripping the entire layer of the lamina propria; contralateral vocal folds were kept intact as normal controls. Six months after the procedures, hydrogels (0.5 mL) containing 1 lg of HGF were injected into the scarred vocal folds of four dogs (HGF-treated group). Hydrogels containing saline solution were injected into the other four dogs (sham group). Histological and vibratory examinations on excised larynges were completed for each group 9 months after the initial surgery.Results: Experiments conducted on excised larynges demonstrated significantly better vibrations in the HGF-treated group in terms of mucosal wave amplitude. Although phonation threshold pressure was significantly lower in the HGF-treated group compared with the sham group, no significant differences were observed in the normalized glottal gap between HGF-treated and sham groups. Histological examinations of the HGF-treated vocal folds showed reduced collagen deposition and less tissue contraction with favorable restoration of hyaluronic acid.Conclusions: Results suggest that administration of HGF may have therapeutic potential in the treatment of chronic vocal fold scarring.

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“…Rosseau and Thibeault 22,23 studied the scarring process of lamina propria and noticed that pro-collagen was more present in the 2nd month postoperative, with stabilization of levels in the 6th month, the same period in which there were higher density collagen fibers manifested on the surgical wound.…”

Section: Discussionmentioning

confidence: 99%

Histologia da camada superficial da lâmina própria da prega vocal ao se aplicar retalho pediculado de mucosa: estudo experimental em cães

Varela¹,

Grellet

2005

Rev. Bras. Otorrinolaringol.

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Many techniques were applied to treat patients with sulcus vocalis and scarred vocal folds. Their results were not good enough. In the Technique of Vocal Fold Pediculated Mucosa Flap, an anterior pediculated flap of vocal fold is positioned on the superficial layer of the lamina propria, below the free margin. Aim: To describe histological postoperative findings on the superficial layer of lamina propria during the application of the technique Vocal Fold Pediculated Mucosa Flap. The following parameters were compared between tested and control groups: total, type I and type III collagen and number of cellular nucleus. Study design: experimental. Material and Method: Fifteen dogs were used. One vocal fold was submitted to the intervention and the other was left as control. Each group of three dogs was sacrificed on 10, 30, 90, 180 and 360 days after the experimental surgery. Hematoxylin and eosin (H.E.) and Syrius Red were the staining techniques used. Results: Type I and total collagen suggested increased results in the tested group on postoperative days 90 and 180, nevertheless there was statistical significance only on postoperative day 180 (p<0.05). Type III collagen group area was less significant than the control group on postoperative day 180 (p<0.05). The number of cellular nucleus was increased on the 10th postoperative day, but decreased after the 30th day. Discussion: The findings about total and type I collagen and the amount of cellular nucleus on the superficial layer of lamina propria were similar to laryngeal postoperative studies in dogs. More complex studies would contribute with new data about the present subject.

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Characterization of Vocal Fold Scarring in a Canine Model

Cited by 191 publications

References 15 publications

Histologic Characterization of Human Scarred Vocal Folds

Histologic Characterization of Human Scarred Vocal Folds

Chronic vocal fold scar restoration with hepatocyte growth factor hydrogel

Histologia da camada superficial da lâmina própria da prega vocal ao se aplicar retalho pediculado de mucosa: estudo experimental em cães

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