Characterization of Vibrio cholerae O139 of an Aquatic Isolate in Northern Vietnam

Nguyen, Doan; Ngo, Tuan Cuong; Tran, Huy Hoang; Le, Thanh Huong; Nguyen, Hoai Thu; Nguyen, Binh Minh; Tran, Nhu Duong; Yamashiro, Tetsu; Ehara, Masahiko

doi:10.2174/1874285801206010014

Cited by 9 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MakA (motility associated killing factor A) is a agella-mediated toxin secreted via the fT3SS. It was rst described in 2018 as pathogenic in Caenorhabditis elegans and zebra sh later reported from Tanzania in reservoirs of Vibrio cholera O1 strains and from epidemics occurring 2015-2017 and also found in sequenced strains from Uganda O1 cholera outbreaks 2014-2016 (55)(56)(57)(58). We conclude that the combination of virulence factors of our isolate suggests the nding of a rough variant of a nontoxigenic Vibrio cholerae O139 rather than a O22 serogroup version.…”

Section: Resultssupporting

confidence: 49%

A rough variant of nontoxigenic Vibrio cholerae O139 imported from Vietnam

Westerström

Dragsted

2022

Preprint

View full text Add to dashboard Cite

Purpose (stating the main purposes and research question) Vibrio cholerae is classified in O-antigen polysaccharide outer membrane properties where O1 and O139 are strains that cause pandemics and epidemics while non-O1/non-O139 usually cause mild disease. The dynamic evolution of Vibrio cholerae involves new virulence factors through horizontal gene transfer and formerly nontoxigenic serogroups are being reported as causing increasingly severe forms of human disease. Our purpose was to investigate serogroup and virulence factors in one imported isolate from Vietnam and compare these to virulence factors seen in different strains of Vibrio cholerae. Methods This is an observational study where we have serotyped one isolate of imported Vibrio cholerae from Vietnam to Denmark and performed whole genome sequencing to identify known virulence genes and furthermore studied the epidemiology of virulence in pathogenic strains of Vibrio cholerae. Results We have identified virulence factors in our isolate that are recently discovered and one toxin, MakA, has not previously been reported from an Asian strain. The isolate was found to be a serogroup non-O1/non-O139 strain, however, sequence analysis gave a 96, 6% ID match of the wbfZ gene, stipulating our isolate to belong to either serogroup O22 or O139. Conclusions Looking at the combination of virulence factors of the isolate we suggest the finding of a rough variant of a nontoxigenic Vibrio cholerae O139 rather than a O22 serogroup version. This is a rare finding in a clinical isolate.

show abstract

Section: Resultssupporting

confidence: 49%

A rough variant of nontoxigenic Vibrio cholerae O139 imported from Vietnam

Westerström

Dragsted

2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The MakA (motility associated killing factor A) is a agella-mediated toxin secreted via the fT3SS. It was rst described in 2018 as pathogenic in Caenorhabditis elegans and zebra sh later reported from Tanzania in reservoirs of Vibrio cholera O1 strains and from epidemics occurring 2015 -2017 and also found in sequenced strains from Uganda O1 cholera outbreaks 2014 -2016 (55)(56)(57)(58). We conclude that the combination of virulence factors of our isolate suggests the nding of a rough variant of a nontoxigenic Vibrio cholerae O139 rather than a O22 serogroup version (Figure 2).…”

Section: Discussionsupporting

confidence: 51%

A Rough Variant of Nontoxigenic Vibrio Cholerae O139 Imported From Vietnam

Westerström

Dragsted

2021

Preprint

View full text Add to dashboard Cite

Purpose (stating the main purposes and research question)Vibrio cholerae is classified in O-antigen polysaccharide outer membrane properties where O1 and O139 are strains that cause pandemics and epidemics while non-O1/non-O139 usually cause mild disease. The dynamic evolution of Vibrio cholerae involves new virulence factors through horizontal gene transfer and formerly nontoxigenic serogroups are being reported as causing increasingly severe forms of human disease. Our purpose was to investigate serogroup and virulence factors in one imported isolate from Vietnam and compare these to virulence factors seen in different strains of Vibrio cholerae.MethodsWe have serotyped one isolate of imported Vibrio cholerae from Vietnam to Denmark and performed whole genome sequencing to identify known virulence genes. ResultsWe have identified virulence factors in our isolate that are recently discovered and one toxin, MakA, has not previously been reported from an Asian strain. The isolate was found to be a serogroup non-O1/non-O139 strain, however, sequence analysis gave a 96, 6% ID match of the wbfZ gene, stipulating our isolate to belong to either serogroup O22 or O139. ConclusionsLooking at the combination of virulence factors of the isolate we suggest the finding of a rough variant of a nontoxigenic Vibrio cholerae O139 rather than a O22 serogroup version. This is a rare finding in a clinical isolate.

show abstract

“…cholera isolations from the swab culture, environmental samples, to identify the genomic region encoding the O1/O139 genotypes, and to detect cholera toxins and other toxin-specific genes, specifically ctxA , ctxB , rtxC , and rstC , using previously published primers [ 24 – 28 ] according to the PCR method described by Nguyen et al . [ 29 ]. A 9 μl of amplicon was separated by electrophoresis on a 1.5% agarose gel and visualized with an ultraviolet light on the Gel Doc System (Bio-Rad Laboratories).…”

Section: Methodsmentioning

confidence: 99%

Cholera returns to southern Vietnam in an outbreak associated with consuming unsafe water through iced tea: A matched case-control study

Nguyen¹,

Pham²,

Quoc³

et al. 2017

PLoS Negl Trop Dis

View full text Add to dashboard Cite

BackgroundAfter more than a decade of steadily declining notifications, the number of reported cholera cases has recently increased in Vietnam. We conducted a matched case-control study to investigate transmission of cholera during an outbreak in Ben Tre, southern Vietnam, and to explore the associated risk factors.Methodology/Principal findingsSixty of 71 diarrheal patients confirmed to be infected with cholera by culture and diagnosed between May 9 and August 3, 2010 in Ben Tre were consecutively recruited as case-patients. Case-patients were matched 1:4 to controls by commune, sex, and 5-year age group. Risk factors for cholera were examined by multivariable conditional logistic regression. In addition, environmental samples from villages containing case-patients were taken to identify contamination of food and water sources. The regression indicated that drinking iced tea (adjusted odds ratio (aOR) = 8.40, 95% confidence interval (CI): 1.84–39.25), not always boiling drinking water (aOR = 2.62, 95% CI: 1.03–6.67), having the main source of water for use being close to a toilet (aOR = 4.36, 95% CI: 1.37–13.88), living with people who had acute diarrhea (aOR = 13.72, 95% CI: 2.77–67.97), and little or no education (aOR = 4.89, 95% CI: 1.18–20.19) were significantly associated with increased risk of cholera. In contrast, drinking stored rainwater (aOR = 0.17, 95% CI: 0.04–0.63), eating cooked seafood (aOR = 0.27, 95% CI: 0.10–0.73), and eating steamed vegetables (aOR = 0.22, 95% CI: 0.07–0.70) were protective against cholera. Vibrio cholerae O1 Ogawa carrying ctxA was found in two of twenty-five river water samples and one of six wastewater samples.Conclusions/SignificanceThe magnitude of the cholera outbreak in Ben Tre was lower than in other similar settings. This investigation identified several risk factors and underscored the importance of continued responses targeting cholera prevention in southern Vietnam. The association between drinking iced tea and cholera and the spread of V. cholerae O1, altered El Tor strains warrant further research. These findings might be affected by a number of limitations due to the inability to capture asymptomatic or mildly symptomatic infections, the possible underreporting of personal unhygienic behaviors, and the purposive selection of environmental samples.

show abstract

Characterization of Vibrio cholerae O139 of an Aquatic Isolate in Northern Vietnam

Cited by 9 publications

References 28 publications

A rough variant of nontoxigenic Vibrio cholerae O139 imported from Vietnam

A rough variant of nontoxigenic Vibrio cholerae O139 imported from Vietnam

A Rough Variant of Nontoxigenic Vibrio Cholerae O139 Imported From Vietnam

Cholera returns to southern Vietnam in an outbreak associated with consuming unsafe water through iced tea: A matched case-control study

Contact Info

Product

Resources

About