Characterization of vegetation and the arbuscular mycorrhizal fungi community in soils impacted by gold mining in Colombia

Abstract

Mining extraction has a substantial impact on soil and vegetation cover. However, little is known about its effect on the flora and microorganisms associated with these environments. Arbuscular Mycorrhizal Fungi (AMF) form associations with plants, improving their development in environments contaminated with heavy metals, among other benefits. Native plants and AMF found in the soil degraded by mining play an essential role in establishing programs dealing with recovering areas influenced by these activities. Therefore, this study aimed to characterize vegetation and AMF abundance and diversity in an area affected by gold mining in Colombia. Vegetation was evaluated in two areas, one adjacent to mining deposits with native vegetation (SI-N) and the other in an intermediate area with mining waste and initial natural plant successions (SI-SV). AMF was assessed in the SI-N and the mining waste area (SI-M). Vegetation was quantified using the RAP method, and AMF was assessed using wet sieving from direct soil samples and trap crops. Vegetation evaluations identified 605 individuals belonging to 29 families, 46 genera, and 54 species, and AMF assessment identified 11 species and five genera. Diversity was established using Hill numbers, finding that SI-N was more diverse for vegetation (Q1: 11.94) and SI-M for AMF (Q1: 3.07). Heavy metals and vegetation decreased the diversity and abundance of AMF in the SI-M. Plant species of the genera Baccharis, Agrostis, Miconia, and the AMF Glomus microcarpum were the most frequent species with potential for recovery in degraded areas.