Characterization of vasopressin receptors in cultured cells derived from the region of rat brain circumventricular organs

Jurzak, Mirek; Müller, Andreas R.; Gerstberger, Rüdiger

doi:10.1016/0306-4522(94)00539-h

Cited by 51 publications

(31 citation statements)

References 51 publications

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“…These structures surround the third ventricle and play an important role in the control of body fluid osmolarity (19). Also, VP terminals and V 1 receptors are present in both of these structures (1,5,20,38).…”

Section: Discussionmentioning

confidence: 99%

Brain vasopressin and sodium appetite

Flynn

Kirchner

Clinton

2002

American Journal of Physiology-Regulatory, Integrative and Comp

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ventricular injections of vasopressin (VP) and antagonists with varying degrees of specificity for the VP receptors were used to identify the action of endogenous brain VP on 0.3 M NaCl intake by sodium-deficient rats. Lateral ventricular injections of 100 ng and 1 g VP caused barrel rotations and a dramatic decrease in NaCl intake by sodium-deficient rats and suppressed sucrose intake. 8 ]VP (MeT-AVP) significantly suppressed NaCl intake by sodium-deficient rats without causing motor disturbances. MeT-AVP had no effect on sucrose intake (0.1 M). In contrast, the selective V2 receptor antagonist had no significant effect on NaCl intake. Last, injections of 100 ng MeT-AVP decreased mean arterial blood pressure (MAP), whereas 100 ng VP elevated MAP and pretreatment with MeT-AVP blocked the pressor effect of VP. These results indicate that the effects produced by 100 ng MeT-AVP represent receptor antagonistic activity. These findings suggest that the effect of exogenous VP on salt intake is secondary to motor disruptions and that endogenous brain VP neurotransmission acting at V1 receptors plays a role in the arousal of salt appetite. neurohypophysial peptides; taste; blood pressure; V 1 receptor; V2 receptor VASOPRESSIN (VP) has diverse biological actions and target systems. In addition to the well-known neuroendocrine effects, VP meets the criteria for a brain neurotransmitter. For example, it is synthesized in several brain nuclei, including the paraventricular nucleus of the hypothalamus, medial amygdala, and bed nucleus of the stria terminalis (1, 5). Also, physiological stimuli trigger the release of VP in specific brain regions where it exerts its effects on pre-and postsynaptic membranes (11, 31).VP exerts its effects through receptors that have been divided into two broad classes, the V 1 and V 2 receptors. The V 1 class can be further divided into the V 1a and V 1b receptors (16,17,42). The receptors differ in their distribution and associated second messenger system (17). Neurons that synthesize VP have extensive vasopressinergic projections to sites along the neuraxis, including the septum, hippocampus, amygdala, subfornical organ (SFO), organum vasculosum of the lamina terminalis (OVLT), nucleus of the solitary tract, dorsal motor nucleus of the vagus, and spinal cord (1,29,40). Radioligand receptor autoradiography and in situ hybridization results indicate that VP binding sites are distributed in the brain and that the distribution of these sites often overlaps with the terminal distributions of vasopressinergic neurons (22,32,46).Brain release and pituitary release of VP often parallel one another. For example, systemic osmotic and hypovolemic stimuli that increase circulating VP levels also stimulate the release of VP in the lateral septum and lateral ventricle (4). Manipulations that facilitate salt intake can also stimulate the systemic release of VP. The simultaneous administration of furosemide and a low dose of the angiotensin-converting enzyme captopril arouses a sodium appetite and increases ...

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Section: Discussionmentioning

confidence: 99%

Brain vasopressin and sodium appetite

Flynn

Kirchner

Clinton

2002

American Journal of Physiology-Regulatory, Integrative and Comp

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“…(Siga et al 1994). In addition, many neurones cultured from the organum vasculosum of the lamina terminalis also exhibited an increase in [Ca2+I1 after application of dDAVP (Jurzak et al 1995). However, receptor binding and in situ hybridisation studies have failed to detect any V, receptor or mRNA in the rat central nervous system in general (see Barberis & Tribollet, 1996) or in magnocellular neurones in particular (Hurbin et al 1998).…”

Section: Via-and V-type Vasopressin Receptor Agonist-induced [Ca2+]mentioning

confidence: 99%

Intracellular calcium signalling in magnocellular neurones of the rat supraoptic nucleus: understanding the autoregulatory mechanisms

Dayanithi

Sabatier

Widmer

2000

Experimental Physiology

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Oxytocin and vasopressin, released at the soma and dendrites of neurones, bind to specific autoreceptors and induce an increase in [Ca2+],. In oxytocin cells, the increase results from a mobilisation of Ca2+ from intracellular stores, whereas in vasopressin cells, it results mainly from an influx of Ca2+ through voltagedependent channels. The response to vasopressin is coupled to phospholipase C and adenylyl-cyclase pathways which are activated by V, (Via and V1J-and V,-type receptors respectively. Measurements of [Ca"], in response to V,, and V, agonists and antagonists suggest the functional expression of these two types of receptors in vasopressin neurones. The intracellular mechanisms involved are similar to those observed for the action of the pituitary adenylyl-cyclase-activating peptide (PACAP). Isolated vasopressin neurones exhibit spontaneous [Ca2+], oscillations and these are synchronised with phasic bursts of electrical activity. Vasopressin modulates these spontaneous [Ca2+I1 oscillations in a manner that depends on the initial state of the neurone, and such varied effects of vasopressin may be related to those observed on the electrical activity of vasopressin neurones in vivo. Experimental Physiology (2000) 85S, 75-84s.Oxytocin neurones show high frequency, synchronised bursts of activity associated with suckling-induced milk ejection, whereas vasopressin neurones exhibit 'phasic' activity characterised by a succession of periods of bursts and silence.

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“…blood pressure [7], High densities of ANG [10], endothelin [11], VP [12], and natriuretic peptide [13] receptors have all been localised within the SFO. Extracellular single unit recordings have shown effects of these peptides on the firing patterns of SFO neurons both in vivo [14,15] and in vitro [16].…”

Section: Introductionmentioning

confidence: 99%

Dissociated Adult Rat Subfornical Organ Neurons Maintain Membrane Properties and Angiotensin Responsiveness for up to 6 Days

et al. 1997

Neuroendocrinology

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We have utilised standard dissociation techniques to obtain a preparation of subfornical organ (SFO) cells that have been maintained in tissue culture for up to 1 week. Stable (>15 min) whole cell recordings were obtained from 80 cells displaying rapid (<2 ms) voltage-dependent sodium currents (blocked by tetrodotoxin in 10 of 10 cells tested), and current evoked action potentials, which were thus classified as SFO neurons. These neurons had a resting membrane potential of-6 3.8 ± 1.3 mV (mean ± SEM), spike amplitude of 86.8 ± 2.5 mV, and input resistance of 1.2 ± 0.1 GΩ, characteristics which did not change significantly in recordings obtained for up to 6 days after dissociation. Current clamp recording showed that of 65 cells tested with bath application of angiotensin (ANG; 1,000^–10 nM), 41 responded to this peptide with decreases in input resistance (control 1.4 ± 0.16 GΩ, after ANG 0.78 ± 0.1 GΩ, p < 0.0001), and depolarisations (mean 18.3 ± 2.0 mV, p < 0.0001). Similar recordings were obtained from viable cells up to 6 days after initial cell dissociation. These studies provide the first description of the basic membrane properties of dissociated SFO neurons. The responsiveness of these cells to ANG supports the conclusion that their properties are similar to those in vivo. These data suggest that use of this technique will permit systematic analysis of the membrane events underlying the actions of multiple ligands on this uniquely specialised group of CNS neurons.

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Characterization of vasopressin receptors in cultured cells derived from the region of rat brain circumventricular organs

Cited by 51 publications

References 51 publications

Brain vasopressin and sodium appetite

Brain vasopressin and sodium appetite

Intracellular calcium signalling in magnocellular neurones of the rat supraoptic nucleus: understanding the autoregulatory mechanisms

Dissociated Adult Rat Subfornical Organ Neurons Maintain Membrane Properties and Angiotensin Responsiveness for up to 6 Days

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