Characterization of vancomycin resistant Enterococcus faecium from clinical and chicken sources

Peter, A.; Radhakrishnan, E. K.; Mathew, Jyothis; Zacharia, Shini

doi:10.1016/s2221-1691(12)60487-0

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…from the gut of Bombyx mori silkworms demonstrated that the use of Mg 2+ ions, at a concentration of 3.5 mM, 15 mM of primer, 200 mM dNTPs, 50 ng DNA template, 3U Taq polymerase in 25 µL of reaction mixture with 45 PCR cycles, resulted in the highest amplification effect [ 25 ]. However, an optimization of the amplification reaction was not the purpose of the present study, and it is worth highlighting that there is a significant variation between clinical VREfm isolates and environmental strains [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

Agarose Gel Electrophoresis-Based RAPD-PCR—An Optimization of the Conditions to Rapidly Detect Similarity of the Alert Pathogens for the Purpose of Epidemiological Studies

Bogiel

Mikucka

Kanarek

2022

Gels

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Agarose gel electrophoresis is a well-known tool to detect DNA fragments amplified in polymerase chain reaction (PCR). Its usefulness has also been confirmed for epidemiological studies based on restriction fragments length polymorphism (RFLP), usually performed using pulsed-field gel electrophoresis (PFGE). Little is known on the effectiveness for alert-pathogen epidemiological studies of another less time-consuming and costly technique called randomly amplified polymorphic DNA-PCR (RAPD-PCR). Meanwhile, its usefulness is believed to be comparable to RFLP-PFGE. Therefore, the aim of the study was to establish and optimize the conditions of agarose gel electrophoresis following RAPD-PCR for 19 Enterococcus faecium strains derived from epidemic outbreaks at intensive care units. An application of different PCR primers, primer combinations, and, in particular, agarose gel concentrations and electrophoresis conditions revealed the usefulness of this relatively fast and inexpensive method based on RAPD-PCR for epidemiological studies without a compulsion to use the specialized equipment necessary for RFLP-PFGE.

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Section: Resultsmentioning

confidence: 99%

Agarose Gel Electrophoresis-Based RAPD-PCR—An Optimization of the Conditions to Rapidly Detect Similarity of the Alert Pathogens for the Purpose of Epidemiological Studies

Bogiel

Mikucka

Kanarek

2022

Gels

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“…Crop and intestinal content of chickens and turkey as well as fish intestinal content samples were collected and prepared as previously described [16]. While the samples from liver, ovary, meat or muscles of both chickens and turkey were prepared according to Peter et al [17]. Human urine samples from outpatient clinic, Zagazig University Hospitals were collected and prepared as previously mentioned [18].…”

Section: Isolation and Identification Of Enterococcimentioning

confidence: 99%

Phenotypic and Genotypic Identification of Vancomycin Resistant Enterococci from Different Sources

Attia

Gharib

Mohamed

et al. 2017

Zagazig Veterinary Journal

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Enterococci are reservoirs for transmission of the most clinically important antimicrobial resistances such as vancomycin resistance. Therefore, this work aimed to determine the occurrence of enterococci and their respective vancomycine resistance genes (vanA and vanB) from different sources. Two hundred and twenty-four samples from chickens, turkey, fish and human urine, as well as, two types of human food including milk (raw and milk from mastitic animals) and sausage were tested for isolation of Enterococcus species. The isolates were identified morphologically and biochemically using catalase test, sodium chloride tolerance and growth at pH 9.6 and 10-45˚C. The vancomycin resistance profile of the isolates was verified by both disc diffusion and agar dilution methods. The genotypic enterococcal identification at both genus and species levels and their vancomycine resistance genes were also ascertained using PCR amplification of the respective genes for 28 isolates. Enterococci isolation rate was 70% of the examined samples with a higher percentage of vancomycine resistance (53.5%) and the minimum inhibitory concentrations (MICs) ranged from 16 to 512 µg/mL. Molecular identification of 28 enterococcal isolates revealed the dominance of E. faecalis (42.8%) and clarified a higher proportion of vanA (78.5%) and vanB (67.8%) genes. In conclusion, administration of the antimicrobials mainly vancomycin may be considered as a pronounced stress factor in the veterinary and human practices. In addition, VRE can act as a reservoir for vancomycin resistance.

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Vancomycin-resistant enterococci carriage in an acute Irish hospital

Whelton

Lynch

O’Reilly

et al. 2016

Journal of Hospital Infection

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Characterization of vancomycin resistant Enterococcus faecium from clinical and chicken sources

Cited by 3 publications

References 20 publications

Agarose Gel Electrophoresis-Based RAPD-PCR—An Optimization of the Conditions to Rapidly Detect Similarity of the Alert Pathogens for the Purpose of Epidemiological Studies

Agarose Gel Electrophoresis-Based RAPD-PCR—An Optimization of the Conditions to Rapidly Detect Similarity of the Alert Pathogens for the Purpose of Epidemiological Studies

Phenotypic and Genotypic Identification of Vancomycin Resistant Enterococci from Different Sources

Vancomycin-resistant enterococci carriage in an acute Irish hospital

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