Characterization of vaginal microbiota of endometritis and healthy sows using high-throughput pyrosequencing of 16S rRNA gene

Wang, Jun; Li, Chang-Jiu; Nesengani, Lucky T.; Gong, Yongsheng; Zhang, Shumin; Lu, Wenjing

doi:10.1016/j.micpath.2017.08.030

Cited by 44 publications

(63 citation statements)

References 19 publications

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“…A study by Xiaojing Xia et al reported that the pathogenic protein secreted by Streptococcus can escape host phagocytosis and complement-mediated immune destruction leading to the onset of the body [6]. Moreover, Wang et al reported that Clostridium_sensu_stricto_1, Fusobacterium and Bacteroides are more abundant in the vagina of endometritis sows compared to healthy sows [7]. Similar results have been obtained in studies of human bacterial vaginosis, compared with healthy women, Bacteroidetes, Actinobacteria and Fusobacteria were more abundant in bacterial vaginosis women [8].…”

Section: Discussionmentioning

confidence: 99%

“…While, we observed obvious statistically predominance of Lactobacillus in 9 OTUs unique to HV samples. Firmicutes was reported to have been the highest abundant phenotype in birth canals samples of the healthy sows [7], and a few members of this phylum are considered to adjust systemic immune responses [9]. Therefore, these bene cial bacteria may involve in regulating bacteria balance, inhibiting conditional pathogens, and preventing colonization of pathogenic microorganisms.…”

Section: Discussionmentioning

confidence: 99%

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Effect of intestinal flora on the formation of endometritis of sow

Zhang¹,

Wang²,

Dai³

et al. 2020

Preprint

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Background Aberration of birth canal microbiota is one of the most important factors in the etiology of sow endomentritis. Nevertheless, reports about the structure and composition of birth canal microbiota in endomentritis sow and their relationship with intestinal microbes is limited. Therefore, understanding the relationship between birth canal microbiota and intestinal microbiota of the host has become exceedingly crucial. Results In this study, 4 healthy and 4 endomentritis sows were selected basing on whether the sow had endometritis or not in a farm of China. The microflora of their birth canal secretions and fresh feces were analyzed via sequencing the V3 + V4 region of bacterial 16S rDNA gene. The results showed that the significant difference between endometritis and healthy sows birth canal flora in the composition and abundance. Sow endomentritis was associated with increasing in the relative abundance of Porphyromonas, Clostridium_sensu_stricto_1, Streptococcus, Ezakiella, Fusobacterium, Actinobacillus, Bacteroides, and Prevotella as well as Anaerococcus. On the contrary, the majority of beneficial bacteria that belonging to Firmicutes phylum (e.g., Lactobacillus and Enterococcus ) declined in endomentritis sow. The increased relative abundance of Porphyromonas in the vaginal secretions might correlate with the decrease of Lactobacillusin the feces of endometritis sows. Moreover, the experimental result also found that some intestinal bacteria (such as Escherichia-Shigella and Bacteroides) may be bound up with the onset of sow endometritis. Conclusion Sow endometritis is closely related to the microbiota of birth canal, and that some intestinal bacteria may promote the onset of endometritis. The above results can supply a theoretical basis to research the pathogenesis of endometritis and the microbiota of sow's birth canal and gut.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of intestinal flora on the formation of endometritis of sow

Zhang¹,

Wang²,

Dai³

et al. 2020

Preprint

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“…Commercial egg producers have special interest in hen oviducts because pathological changes or disrupted activity directly affect egg production efficiency and ultimately decrease economic profitability [92]. A previous study demonstrated that vaginal microbiota of sows could affect endometritis [93], with endometritis, the proportion of the smallest follicles was only 60% [94]. The isthmus in chicken forms both inner and outer shell membranes, while calcification of the eggshell and its subsequent pigmentation and cuticle deposition occur in the uterus, followed by expulsion of the egg through the vagina [95].…”

Section: Host Genetics Has Limited Effect On Chicken Digestive/reprodmentioning

confidence: 99%

Influence of the digestive/reproductive tract microbiota on chicken egg production beyond host genetics

Tian

Zhu

et al. 2020

Preprint

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Background: The microbiota of the digestive and reproductive systems has a prominent role in animal health and performance, but the extent of its contribution is difﬁcult to determine. In chickens, the effect of host genetics on the reproductive and digestive tract microbiota is unclear, and the means by which digestive/reproductive microbiomes help improve egg production in chicken are unknown.Results: To gain insight into this, we examined genomes from 128 chickens reared under identical conditions and described their digestive (crop, gizzard and small intestine) and reproductive tract (vagina, uterus and isthmus) microbiota. Although the diversity, composition and predicted function of the digestive and reproductive tract microbiota exhibited notable microbiota variation substantially between different parts, host genetics had limited effects on the reproductive and digestive tract microbial community. The digestive and reproductive tract microbiota had a significant effect on egg production (accounting for 52.31% - 98.86% of the variance), after correcting for host genetic effects; in particular, the uterus and isthmus microbiota accounted for an average of 93.59% and 98.86%, respectively, of variance in egg production. We further identiﬁed four reproductive tract microbial species which were related to immune system, Bacteroides fragilis, Bacteroides salanitronis, Bacteroides barnesiae and Clostridium leptum, that were signiﬁcantly positively correlated with egg production. Chickens with a lower abundance of these species had produced signiﬁcantly fewer eggs at 300 days of age (37.13 vs. 113.75) than those with a higher abundance of these microorganisms. These taxa indicate potential roles play in promoting reproductive performance. Especially uterus and isthmus tract microbiota were major factors in regulating the chicken egg production.Conclusions: Host genetics has limited effect on digestive/reproductive microbiome composition. The distinct site-associated chicken microbiome may be determined by the differences of their physical function. These ﬁndings may help design strategies for controlling and altering the digestive/reproductive tract microbiota in chickens to improve egg production.

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“…The most commonly occurring postpartum uterine diseases in domestic animals can be classified as metritis, endometritis and pyometra (Abiven, et al, 1998;Sheldon, et al, 2006;Katila, 2016). Endometritis is one of the major postpartum uterine diseases of females and is considered as the leading cause of infertility and economic loss in the cattle, equine and swine breeding industries (Beutler, et al, 2003;Sheldon, et al, 2006;LeBlanc and Causey, 2009;Canisso, et al, 2016;Wang, et al, 2017). At present, endometritis can be categorized as clinical and subclinical endometritis.…”

Section: Introductionmentioning

confidence: 99%

TREM‐1 deficiency attenuates the inflammatory responses in LPS‐induced murine endometritis

Zhu

Wang

et al. 2019

Microbial Biotechnology

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Summary Endometritis, which is usually caused by bacterial infection, is characterized by high levels of pro‐inflammatory cytokines and a high infertility rate. Triggering receptor expressed on myeloid cells‐1 (TREM‐1) has been recognized as a potent amplifier of inflammatory reactions. Studies have demonstrated reduced inflammatory responses and mortality rates of animals with bacterial infection due to the blocking of TREM‐1 expression. However, whether TREM‐1 deficiency could alleviate the inflammatory reaction in bacterial endometritis is still unclear. Here, TREM‐1 knock‐out (Trem‐1−/−) mice were used to inhibit TREM‐1 signalling to evaluate its role in inflammatory reactions after a highly pathogenic LPS infection in mice uteri. The results demonstrated that TREM‐1 deficiency attenuated the inflammation in mice uteri; markedly reduced the number of polymorphonuclear neutrophils; and suppressed interleukin‐1β (IL‐1β), IL‐6, and tumour necrosis factor‐α (TNF‐α) concentrations in serum as well as their production in inflamed uteri after LPS stimulation. Our results illustrate an anticipated pathogenic impact of TREM‐1 on endometritis during LPS infection and indicate that blocking of TREM‐1 in LPS‐induced endometritis holds considerable promise for blunting excessive inflammation.

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Characterization of vaginal microbiota of endometritis and healthy sows using high-throughput pyrosequencing of 16S rRNA gene

Cited by 44 publications

References 19 publications

Effect of intestinal flora on the formation of endometritis of sow

Effect of intestinal flora on the formation of endometritis of sow

Influence of the digestive/reproductive tract microbiota on chicken egg production beyond host genetics

TREM‐1 deficiency attenuates the inflammatory responses in LPS‐induced murine endometritis

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