Characterization of vaccinia virus particles using microscale silicon cantilever resonators and atomic force microscopy

Johnson, Luke A.; Gupta, Amit; Ghafoor, A.; Akin, Demir; Bashir, Rashid

doi:10.1016/j.snb.2005.08.047

Cited by 98 publications

(73 citation statements)

References 23 publications

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“…In addition, the measurement of the resonant frequency of these devices in liquid is scientifically and The device mass and the detected mass levels are plotted as empty and filled symbols, respectively. The data split into measurements in vacuum [115][116][117] (red), air 94,[118][119][120] (green) and liquid 107,[121][122] (blue). In air and vacuum, the measurements are usually performed ex-situ before and after the biological assay to attain the highest sensitivity in the resonance frequency due to the high quality factor.…”

Section: Biosensors Based On Nanomechanical Resonatorsmentioning

confidence: 99%

Biosensors based on nanomechanical systems

Tamayo¹,

Kosaka²,

Ruz³

et al. 2013

Chem. Soc. Rev.

350

239

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The advances in micro-and nanofabrication technologies are enabling increasingly smaller mechanical transducers capable of detecting the forces, motion, mechanical properties and masses that emerge in biomolecular interactions and fundamental biological processes. Thus, biosensors based on nanomechanical systems have gained considerable relevance in the last decade. This review provides insight into the mechanical phenomena that occur in suspended mechanical structures when either biological adsorption or interactions take place on their surface. This review guides the reader through the parameters that change as a consequence of biomolecular adsorption: mass, surface stress, effective Young's modulus and viscoelasticity.The mathematical background needed to correctly interpret the output signals from nanomechanical biosensors is also outlined here. Other practical issues reviewed are the immobilization of bioreceptor molecules on the surface of nanomechanical sensors and methods to attain that in large arrays of sensors. We describe then some relevant realizations of biosensor devices based on nanomechanical systems that harness some of the mechanical effects cited above. We finally discuss the intrinsic detection limits of the devices and the limitation that arises from non-specific adsorption.2

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Section: Biosensors Based On Nanomechanical Resonatorsmentioning

confidence: 99%

Biosensors based on nanomechanical systems

Tamayo¹,

Kosaka²,

Ruz³

et al. 2013

Chem. Soc. Rev.

350

239

View full text Add to dashboard Cite

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“…The mass of a single virus is approximately 9 fg. (9,10) Therefore, the adhered virus mass in the electroded region was calculated and denoted as Δm = 189.9 ng. By substituting Δm into Eq.…”

Section: Resultsmentioning

confidence: 99%

Correction of Mass Effect of Nonelectroded Region of a Quartz Crystal Microbalance (QCM) in Ultra-Micromass Measurement

2016

Sensors and Materials

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Keywords: mass effect of nonelectroded region, QCM, correctionWhen applying a quartz crystal microbalance (QCM) to ultra-micromass measurement, not only the electroded region but also the nonelectroded region absorb analytes. The mass loading on the sensing surface of the QCM chip (with both electroded and nonelectroded regions) causes a shift in the frequency of the QCM. A method based on the integral of the mass sensitivity function is presented in this paper which can distinguish between the frequency shift caused by the electroded region and that caused by the nonelectroded region. Finally, the feasibility of the correction method can be confirmed on the basis of the data processing results of an airborne Vaccinia virus testing experiment using a QCM.

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“…The mass of a single E. coli O157:H7 cell was found by the authors to be 665 fg. Similarly, Johnson et al reported the use of microscale silicon cantilever resonators for vaccinia virus detection in air [53]. The authors measured the mass of a single vaccinia virus particle to be 12.4 ± 1.3 fg and 7.9 ± 4.6 fg using two different-sized cantilever beams [51].…”

Section: Microcantilever-based Biosensorsmentioning

confidence: 99%

Microbiological Detectors for Food Safety Applications

Alocilja

Pal

2008

Wiley Handbook of Science and Technology for Homeland Security

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The use of microorganisms as biological weapons has long been reported in history. The most recent was the deliberate release of B. anthracis spores through the postal system in the United States in October 2001, resulting in 22 cases of anthrax and five deaths. Furthermore, there are up to 33 million cases of human illness each year from microbial pathogens in the food supply in the United States alone, with an associated cost of $2‐4 billion in 2006. The complexity of the US food supply chain from cradle to grave provides numerous entry points and routes in which (inadvertent and intentional) contaminants and pathogens can be introduced into the nation's food system. Many of these pathogenic microorganisms have a very low infectious dose to cause fatality. The most vulnerable populations are the very young, the elderly, and immunocompromised individuals. Although highly sensitive, current methods of detection are laborious, require highly skilled personnel, and typically require 2–7 days to obtain conclusive results. Their results are not available in the time‐scale desired in food defense, food quality assurance, or clinical laboratory procedures, which have safety, cost, and quality implications. Rapid on‐site point‐of‐care detection methods for pathogens have hence become a necessity. Biosensors can play a major role in addressing the combined needs for near real‐time diagnosis, specificity, sensitivity, portability, electronic data transmission, field‐based, ease of use, and low cost requirements. This chapter presents biosensors for rapid microbial detection in complex food matrices of concern to homeland security and food safety.

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Characterization of vaccinia virus particles using microscale silicon cantilever resonators and atomic force microscopy

Cited by 98 publications

References 23 publications

Biosensors based on nanomechanical systems

Biosensors based on nanomechanical systems

Correction of Mass Effect of Nonelectroded Region of a Quartz Crystal Microbalance (QCM) in Ultra-Micromass Measurement

Microbiological Detectors for Food Safety Applications

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