Characterization of uraninite nanoparticles produced by Shewanella oneidensis MR-1

Burgos, William D.; McDonough, Jeffrey T.; Senko, John M.; Zhang, Gengxin; Dohnálková, Alice; Kelly, Shelly D.; Gorby, Yuri A.; Kemner, Kenneth M.

doi:10.1016/j.gca.2008.07.016

Cited by 123 publications

(124 citation statements)

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“…One promising strategy for the remediation of uranium aims at transforming the soluble and mobile hexavalent form of uranium, U(VI), to the reduced and relatively immobile tetravalent form, U(IV) (O'Loughlin et al, 2003;Jeon et al, 2005;Wall and Krumholz, 2006;Burgos et al, 2008;Sheng et al, 2011;Zhang et al, 2011 reduction of U(VI) to U(IV) was only found to produce the sparingly soluble mineral uraninite, UO 2(s) (Lovley et al, 1991;Lovley and Phillips, 1992;Lovley, 1993;Burns, 1999;O'Loughlin et al, 2003;Wall and Krumholz, 2006;Burgos et al, 2008). However, recent research reveals that non-uraninite species of U(IV), i.e., those lacking the 3.85 Å U-U pair correlation characteristic of UO 2 observed using X-ray absorption spectroscopy (XAS), can form as the product of U(VI) reduction by Gram-negative and Gram-positive bacteria (Bernier-Latmani et al, 2010;Fletcher et al, 2010;Boyanov et al, 2011;Cologgi et al, 2011;Ray et al, 2011;Sivaswamy et al, 2011), by biogenic Fe(II)-bearing minerals (Veeramani et al, , 2013Latta et al, 2012), and in biostimulated or naturally reduced sediments (Campbell et al, 2011;Sharp et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

The product of microbial uranium reduction includes multiple species with U(IV)–phosphate coordination

Alessi

Lezama‐Pacheco

Stubbs

et al. 2014

Geochimica et Cosmochimica Acta

115

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Until recently, the reduction of U(VI) to U(IV) during bioremediation was assumed to produce solely the sparingly soluble mineral uraninite, UO 2(s) . However, results from several laboratories reveal other species of U(IV) characterized by the absence of an EXAFS U-U pair correlation (referred to here as noncrystalline U(IV)). Because it lacks the crystalline structure of uraninite, this species is likely to be more labile and susceptible to reoxidation. In the case of single species cultures, analyses of U extended X-ray fine structure (EXAFS) spectra have previously suggested U(IV) coordination to carboxyl, phosphoryl or carbonate groups. In spite of this evidence, little is understood about the species that make up noncrystalline U(IV), their structural chemistry and the nature of the U(IV)-ligand interactions. Here, we use infrared spectroscopy (IR), uranium L III -edge X-ray absorption spectroscopy (XAS), and phosphorus K-edge XAS analyses to constrain the binding environments of phosphate and uranium associated with Shewanella oneidensis MR-1 bacterial cells. Systems tested as a function of pH included: cells under metal-reducing conditions without uranium, cells under reducing conditions that produced primarily uraninite, and cells under reducing conditions that produced primarily biomass-associated noncrystalline U(IV). P X-ray absorption near-edge structure (XANES) results provided clear and direct evidence of U(IV) coordination to phosphate. Infrared (IR) spectroscopy revealed a pronounced perturbation of phosphate functional groups in the presence of uranium. Analysis of these data provides evidence that U(IV) is coordinated to a range of phosphate species, including monomers and polymerized networks. U EXAFS analyses and a chemical extraction measurements support these conclusions. The results of this study provide new insights into the binding mechanisms of biomass-associated U(IV) species which in turn sheds light on the mechanisms of biological U(VI) reduction.

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Section: Introductionmentioning

confidence: 99%

The product of microbial uranium reduction includes multiple species with U(IV)–phosphate coordination

Alessi

Lezama‐Pacheco

Stubbs

et al. 2014

Geochimica et Cosmochimica Acta

115

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“…Shewanella putrefaciens interacts with U(VI) reductases and biogenic U(IV) on the cell surface with uranium salt. Uraninite particles accumulate on extracellular polymeric substances [62]. The average particle size was 3 nm, as determined by high-resolution transmission electron microscopy (HRTEM) and X-ray absorption spectroscopy.…”

Section: Uraninite (Uo2) Nanoparticlesmentioning

confidence: 99%

“…The average particle size was 3 nm, as determined by high-resolution transmission electron microscopy (HRTEM) and X-ray absorption spectroscopy. Scanning electron microscopy (SEM) analysis revealed that nanoparticles exhibit extracellular accumulation [62]. The synthesis of biogenic UO2 nanoparticles (5-10 nm) was mediated by S. putrefaciens cell suspensions growing aerobically, followed by the anaerobic addition of a uranyl-bearing solution [(UO2 +2 )-PIPES,NH4Cl-lactate-KHCO3-K2HPO4] [63].…”

Section: Uraninite (Uo2) Nanoparticlesmentioning

confidence: 99%

“…A recent review [112] discussed the importance of bioreduction processes in which bacteria enzymatically reduce aqueous U(VI) (toxic) to insoluble U(IV) (less toxic) coupled with the oxidation of an organic electron donor [112]. Therefore, microorganisms play a key role in the environmental decontamination of soluble U(VI) by its reduction to the poorly soluble mineral uraninite (UO2) [58,59,62,112].…”

Section: Uraninite (Uo2) Nanoparticlesmentioning

confidence: 99%

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Nanotoxicology of Metal Oxide Nanoparticles

Seabra

Durán

2015

Metals

177

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This review discusses recent advances in the synthesis, characterization and toxicity of metal oxide nanoparticles obtained mainly through biogenic (green) processes. The in vitro and in vivo toxicities of these oxides are discussed including a consideration of the factors important for safe use of these nanomaterials. The toxicities of different metal oxide nanoparticles are compared. The importance of biogenic synthesized metal oxide nanoparticles has been increasing in recent years; however, more studies aimed at better characterizing the potent toxicity of these nanoparticles are still necessary for nanosafely considerations and environmental perspectives. In this context, this review aims to inspire new research in the design of green approaches to obtain metal oxide nanoparticles for biomedical and technological applications and to highlight the critical need to fully investigate the nanotoxicity of these particles. OPEN ACCESSMetals 2015, 5 935

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“…In another example, dissolved calcium was reported to cause a decrease in the rate and extent of bacterial U(VI) reduction (Brooks et al, 2003). Thus, some concentrated cationic ions of groundwater in which U(VI) reduction takes place will undoubtedly exert control on the nature to form biogenic U(IV) phases (Abdelouas et al, 1998;Burgos et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Influence of Iron Phases on Microbial U(VI) Reduction

Lee¹,

Baik²,

Lee³

et al. 2011

Journal of Soil and Groundwater Environment

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The bacterial uranium(VI) reduction and its resultant low solubility make this process an attractive option for removing U from groundwater. An impact of aqueous suspending iron phase, which is redox sensitive and ubiquitous in subsurface groundwater, on the U(VI) bioreduction by Shewanella putrefaciens CN32 was investigated. In our batch experiment, the U(VI) concentration (5×10 -5 M) gradually decreased to a non-detectable level during the microbial respiration. However, when Fe(III) phase was suspended in solution, bioreduction of U(VI) was significantly suppressed due to a preferred reduction of Fe(III) instead of U(VI). This shows that the suspending amorphous Fe(III) phase can be a strong inhibitor to the U(VI) bioreduction. On the contrary, when iron was present as a soluble Fe(II) in the solution, the U(VI) removal was largely enhanced. The microbially-catalyzed U(VI) reduction resulted in an accumulation of solid-type U particles in and around the cells. Electron elemental investigations for the precipitates show that some background cations such as Ca and P were favorably coprecipitated with U. This implies that aqueous U tends to be stabilized by complexing with Ca or P ions, which easily diffuse and coprecipitate with U in and around the microbial cell.

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Characterization of uraninite nanoparticles produced by Shewanella oneidensis MR-1

Cited by 123 publications

References 55 publications

The product of microbial uranium reduction includes multiple species with U(IV)–phosphate coordination

The product of microbial uranium reduction includes multiple species with U(IV)–phosphate coordination

Nanotoxicology of Metal Oxide Nanoparticles

Influence of Iron Phases on Microbial U(VI) Reduction

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