Characterization of UDP-galactose:2-acetamido-2-deoxy-D-glucose 3 beta-galactosyltransferase from pig trachea.

Sheares, B T; Carlson, Don M.

doi:10.1016/s0021-9258(17)44582-0

Cited by 43 publications

(2 citation statements)

References 34 publications

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“…transferase that transfers Gal to glucosylceramide producing CDH or to GlcNAc-CTH producing paragloboside (Yamato & Yoshida, 1982), a /31-*3Gal transferase in pig trachea (Sheares & Carlson, 1983), a /31-*3Gal transferase in chick embryonic liver that uses asialo ovine submaxillary mucin as acceptor (Furukawa & Roth, 1985), a /31-*4Gal transferase in chick embryonic liver that transfers Gal to GlcNAc or asialo-agalacto-aracid glycoprotein (Furukawa & Roth, 1985), and a /31-*-3Gal transferase in human kidney that transfers Gal to lactosylceramide (Bailly et al, 1988). The /31-1 3Gal:globoside Gal transferase in our study is presumably analogous to these other /3-Gal transferases.…”

Section: Discussionmentioning

confidence: 99%

Glycolipid glycosyltransferases in human embryonal carcinoma cells during retinoic acid induced differentiation

Chen¹,

Fenderson²,

Andrews³

et al. 1989

Biochemistry

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Retinoic acid induced differentiation of TERA-2-derived human embryonal carcinoma cells is accompanied by a dramatic reduction of extended globo-series glycolipids, including galactosyl globoside, sialylgalactosyl globoside, and globo-A antigen (each recognized by specific MoAbs). Associated with these glycolipid changes, the activities of two key enzymes, alpha 1----4 galactosyltransferase (for synthesis of globotriaosyl core structure) and beta 1----3 galactosyltransferase (for synthesis of galactosyl globoside), were found to be reduced 3- to 4-fold. The latter enzyme plays a key role in the synthesis of extended globo-series structures, and its characterization has not been reported previously. Therefore, its catalytic activity was studied in detail, including substrate specificity, detergent and phospholipid effects, pH and cation requirements, and apparent Km. During retinoic acid induced differentiation, a series of Lex glycolipid antigens (recognized by anti-SSEA-1 antibody) and their core structures (lacto-series type 2 chains) increase dramatically. In parallel with these changes in glycolipid expression, the activities of two key enzymes, beta 1----3 N-acetylglucosaminyltransferase (for extension of lacto-series type 2 chain) and alpha 1----3 fucosyltransferase (for synthesis of Lex structure), were found to increase by 4- and 2-fold, respectively. Similarly, an increase in the expression of several gangliosides (e.g., GD3 and GT3) during retinoic acid induced differentiation was mirrored by a 4-fold increase in the activity of alpha 2----3 sialyltransferase (for synthesis of ganglio core structure, GM3). The results suggest a coordinate regulation of key glycosyltransferases involved in core structure assembly and terminal chain modification.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%

Glycolipid glycosyltransferases in human embryonal carcinoma cells during retinoic acid induced differentiation

Chen¹,

Fenderson²,

Andrews³

et al. 1989

Biochemistry

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“…A strict correlation of age-related changes in the activities of glycosyltransferases and the contents of respective sugars in secreted mucin is not expected, in part because only selected glycosyltransferases were examined. For example, at least four different 0-galactosyltransferases may be involved in the synthesis of tracheal mucins (Cheng & Bona, 1982;Sheares & Carlson, 1983, but only GalNAc:/33 galactosyltransferase was assayed. Differences in the relative activities of the specific glycosyltransferases that utilize the same acceptor may influence the structure as well as the composition of the mucin oligosaccharide chains that are generated.…”

Section: Discussionmentioning

confidence: 99%

Developmental changes of ferret tracheal mucin composition and biosynthesis

Leigh¹,

Cheng²,

Boat³

1989

Biochemistry

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We characterized the chemical composition of mucins secreted by ferret tracheal explants and the activities of key mucin glycosyltransferases in ferret tracheal epithelium during a period of rapid postnatal maturation of the mucin-secreting structures. Ferret tracheal explants secrete three major groups of high molecular weight glycoconjugates: (1) those susceptible to bovine testicular hyaluronidase; (2) those resistant to hyaluronidase and exhibiting high density (p greater than or equal to 1.60 g/mL); and (3) those resistant to hyaluronidase and exhibiting low density (1.45 less than or equal to p less than 1.60 g/mL). The hyaluronidase-resistant, low-density glycoconjugates have typical mucin properties and constitute 36% of total glycoconjugates released in newborns but only 8% in adult ferrets. Mucin secretory rate per unit surface area of trachea progressively decreases with age. Mucin amino acid and total carbohydrate contents do not vary; however, the sialic acid content increases, and fucose content as well as blood group A activity of the mucins decreases with age. Four glycosyltransferases involved in mucin biosynthesis [Gal beta 3GalNAc:(GlcNAc-GalNAc)beta 6 N-acetylglucosaminyl-, GalNAc:beta 3 galactosyl-, Gal:alpha 2 fucosyl-, and GalNAc alpha 2----6 neuraminyltransferase] are present in tracheal epithelium of ferrets at all ages. Activities of all but the neuraminyltransferase decrease with age. The relatively greater neuraminyltransferase activity is consistent with increased incorporation of sialic acid into secreted mucins over the same age span. Conversely, diminution of fucosyltransferase relative to galactosyltransferase activity may contribute to the lower fucose content and lower blood group A activity of mucins secreted by mature ferret tracheas.(ABSTRACT TRUNCATED AT 250 WORDS)

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Glycosyltransferases Involved inN–andO–Glycan Biosynthesis

Brockhausen¹,

Schachter²

1996

Glycosciences

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Characterization of UDP-galactose:2-acetamido-2-deoxy-D-glucose 3 beta-galactosyltransferase from pig trachea.

Cited by 43 publications

References 34 publications

Glycolipid glycosyltransferases in human embryonal carcinoma cells during retinoic acid induced differentiation

Glycolipid glycosyltransferases in human embryonal carcinoma cells during retinoic acid induced differentiation

Developmental changes of ferret tracheal mucin composition and biosynthesis

Glycosyltransferases Involved inN–andO–Glycan Biosynthesis

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